20 results about "Cell signaling pathways" patented technology

The invention discloses a preparation method of an oral liver protection composition containing ergothioneine, and relates to the technical field of ergothioneine application. The oral liver protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.5 to 5 parts of the ergothioneine, 10 to 30 parts of silibinin, 20 to 50 parts of a phospholipid complex, 5 to 15 parts of a liver targeting carrier, 2 to 8 parts of a disintegrating agent, 1 to 5 parts of an adhesive and 2 to 4 parts of an antioxidant. Free radicals are efficiently captured through a polycyclic conjugated pi electron system of the antioxidant, a hydrogen bond network is formed by a heteroatom bridging effect of the antioxidant, a galactose group of the liver targeting carrier and the disintegrating agent, and the stability of the system is synergistically improved; the phospholipid complex is embedded with a hydrophobic drug and a hydrophilic carrier, and the drug is accurately delivered to the liver by combining the specific recognition capability of the liver targeting carrier; the ergothioneine and the silybin form a bidirectional synergistic liver protection mechanism by removing reactive oxygen free radicals and regulating and controlling cell signal channels respectively.

The invention discloses a prediction method for identifying a protein-protein interaction site. According to the method, a deep learning framework is constructed based on protein chain hierarchical graph construction and double-branch graph neural network learning. The method comprises the following steps: firstly, constructing a corresponding hierarchical graph for each protein chain, including a residue-level graph and an atomic-level graph; next, the method constructs an isotropic graph neural network (EGNN) module to extract residue-level embedding with isotropic features. After a GraphSAGE module is further used for extracting atomic-scale embedding, the method integrates layered graph features by using a comparative learning strategy so as to learn residue-scale and atomic-scale consistency embedding. Finally, an improved gated multi-head attention mechanism is used for fusion embedding weighting, and residue-level interaction classification prediction is performed through a multi-layer perceptron. A comprehensive evaluation result on a plurality of data sets shows that the method is remarkably superior to an existing method. In conclusion, according to the method, various advanced technologies such as graph structure denaturation, multi-scale modeling and comparative learning are fused, and an accurate and steady solution with expansion potential is provided for protein interaction prediction. The method can assist researchers in deeply recognizing key protein acting sites and promoting analysis work of cell signal channels and metabolic networks.

The invention discloses a culture method for improving proliferation and differentiation capacity of NK (Natural Killer) cells based on angelica sinensis-astragalus membranaceus exosomes, which comprises the following steps: firstly extracting exosomes in angelica sinensis and astragalus membranaceus, then adding the exosomes into an NK cell culture system according to a specific concentration, and regulating and controlling an NK cell signal channel by virtue of active ingredients carried by the exosomes so as to improve the proliferation and differentiation capacity of the NK cells. Therefore, the proliferation rate and differentiation maturity of the NK cells are remarkably improved. Experimental results show that compared with a traditional culture method, the proliferation multiple of the NK cells cultured through the method is increased by 28.99%-33.69%, the proportion of differentiated mature cells is increased by 15.91%-28.30%, and the cell killing activity is not remarkably reduced. The method can be widely applied to the field of immune cell treatment, provides a high-quality NK cell source for clinic, effectively solves the problems of slow cell proliferation rate, low differentiation maturity, easy activity reduction after large-scale culture and the like in traditional NK cell in-vitro culture, and has important clinical application value and industrialization prospect.

The invention discloses a liver tissue regeneration regulation and control method based on epigenetic state regulation and control, and relates to the technical field of biology. Comprising the following steps: sampling the liver tissue at a plurality of preset time points after the liver tissue is damaged or partially resected, selecting a target gene related to liver tissue regeneration regulation, and detecting an epigenetic state of a promoter region of the target gene; comparing and analyzing the change of the epigenetic state of the target gene promoter region on the basis of detection results obtained at different time points, and determining epigenetic modification characteristics in the liver tissue regeneration process; according to an analysis result, intervening the expression level of a regulatory factor related to DNA methylation or DNA hydroxymethylation so as to regulate the epigenetic state of the target gene promoter region; after intervention is completed, the transcription expression condition of a target gene and the activity change of a cell signal channel related to liver tissue regeneration are detected, and the liver tissue regeneration process can be effectively regulated and controlled through the method.

The present invention discloses a class of flavonoid derivatives, their preparation methods, and applications. The structural formula is shown in Formula I: wherein X is selected from CH and N, and R1 is selected from H. These compounds can significantly inhibit the activation of STAT3, a cell signaling pathway, at low doses. Experimental results show that these compounds can significantly inhibit the proliferation of various tumor cell lines, including lymphoma, multiple myeloma, cervical cancer, breast cancer, gastric cancer, lung cancer, and pancreatic cancer. They can specifically inhibit STAT3 signaling activation and the expression of the downstream target gene cyclinD1, indicating that these compounds have the potential to be developed as targeted anti-tumor drugs related to the STAT3 signaling pathway.

The application discloses a detection kit based on a colorimetric method and application thereof, and the detection kit comprises two membrane protein recognition chains T1 and T2, two DNA hairpins H1 and H2 required for forming G-quadruplex, hematin required for a color developing reaction, hydrogen peroxide (H2O2) and ABTS. The membrane protein recognition chains T1 and T2 can anchor membrane proteins, and adjacent hybridization occurs when the membrane proteins dimerize, thereby triggering catalytic hairpin self-assembly of H1 and H2 to form a large number of G-quadruplex structures; when H2O2 exists, G-quadruplex / hematin formed by combining G-quadruplex and hematin can catalyze oxidation of ABTS to generate a color developing reaction. The detection kit has the characteristics of high sensitivity, good specificity, low cost, low dependence on equipment and simple operation in the detection of cell membrane protein dimerization, and also has good application performance in cell signal pathway visualization and rapid diagnosis of tumor cells at a bedside.

The invention relates to the technical field of gene engineering technology, in particular to a preparation method of an efficient and stable gene knockout cell line. According to the AKAP11 gene knockout cell line provided by the invention, the 6th exon of the AKAP11 gene in the cell line is subjected to 3395bp frameshift deletion mutation, so that a coding frame is shifted, and protein translation is terminated in advance; after continuous subculture, the homozygous knockout phenotype is still maintained through PCR sequencing verification. A CRISPR / Cas9 system is combined with puromycin screening, the positive cloning rate reaches 30% or above, the construction period is shortened to 45 days, the constructed cell line can be directly used for AKAP11 related cell signal path research, disease model construction and targeted drug screening, and a reliable tool is provided for basic research and clinical transformation.

The invention belongs to the technical field of biological medicines, and particularly relates to application of neratinib in preparation of a medicine for preventing and treating noise-induced deafness. According to the specific technical scheme, the application of the neratinib in preparation of the medicine for preventing and treating hearing impairment / deafness is provided. According to the invention, the fact that neratinib can play a hearing protection role by specifically regulating and controlling cochlea immune microenvironment is found for the first time, and the hearing protection role is specifically realized by the following mechanisms: regulating and controlling biological effects such as cell signal channels, inflammatory response and cell stress response, protecting hair cell structures, and reducing cochlea basilar membrane outer hair cell loss caused by noise exposure. Based on the new discovery of the invention, the neratinib can be used for preparing medicines for preventing and treating hearing impairment and deafness caused by noise.

The invention discloses a method for improving proliferation and activity of cytokine-induced killer cells, and belongs to the technical field of cellular immunity. According to the method, the human peripheral blood mononuclear cells are firstly cultured in the culture medium containing the cell factors, then the obtained CIK cells are cultured in the culture medium containing the cell factors and the PP2A inhibitor, the amplification efficiency of the CIK cells is improved through the PP2A inhibitor, and the proportion of CD3 + CD56 + cells in the CIK cells is increased; according to the invention, a cell signal transduction pathway regulator PP2A inhibitor is ingeniously introduced, and culture conditions are optimized, so that the amplification efficiency of CIK cells is remarkably improved, and the proportion of functional subgroups is improved. Meanwhile, on the basis of improving the cell performance, the preparation cost of the CIK cell is relatively reduced, and the CIK cell is particularly suitable for preparing a CIK cell preparation required by adoptive immunotherapy and can be widely applied to treatment of solid tumors and blood system tumors.

The invention relates to a single cell signal road reconstruction method, system and device and a medium. The method comprises the following steps: extracting a sub-network from a global signal reference network based on an intersection of a highly expressed gene and a hypervariable gene of each cell, and generating a cell specific signal network; inputting the cell specificity signal network into a Transform model to generate a consensus signal network; defining the consensus signal network corresponding to the source cell as a source cell consensus network, and defining the consensus signal network corresponding to the target cell as a target cell consensus network; and on the basis of a ligand-receptor knowledge base, searching a path between a ligand in the source cell consensus network and a receptor in the target cell consensus network, and generating a cross-cell signal path list. By adopting the method, the intercellular communication mechanism can be accurately captured, and the systematic understanding of intercellular signals is effectively improved.

The present invention relates to immune cells overexpressing externally introduced cell signaling pathway modulators and uses thereof. As a specific example, immune cells expressing a fusion protein comprising a chimeric antigen receptor and a cell signaling pathway modulator perform an immune response by selecting target cancer cells through the chimeric antigen receptor expressed on the cell membrane. In this case, the cell signaling pathway modulator is overexpressed in the cytoplasm, thereby being able to modulate the activity of the immune cells. Accordingly, the fusion protein comprising a chimeric antigen receptor and a cell signaling pathway modulator of the present invention, and the immune cells overexpressing the cell signaling pathway modulator can be used for the treatment of cancer.

The invention discloses a method for separating porcine reproductive and respiratory syndrome virus and porcine circovirus, and belongs to the technical field of virus separation. The separation method comprises the following steps: preparing a virus positive sample into homogenate, and centrifuging to obtain virus liquid; mixing the virus solution with polypropylene glycol and a cholesterol solution to obtain a virus infection solution; inoculating cells with the virus infection solution, and co-culturing in a virus culture solution; and after co-culture is finished, freezing and thawing the cell culture for three times to serve as an F1 generation, subculturing to an F3 generation according to the steps (1)-(3), and harvesting venom. According to the method, polypropylene glycol and cholesterol are added in the virus separation process, the sensitivity of cells to viruses is improved, noradrenaline is added into a virus culture solution, a cell signal channel is adjusted, virus proliferation and release can be promoted, and the purpose of rapidly separating the viruses is achieved.

The present invention relates to sepsis, and to compounds useful in the prevention and treatment of subjects suffering from sepsis or at risk for developing sepsis. The invention particularly relates to compounds that inhibit the AR cellular signaling pathway activity, or compound that inhibit the TGFbeta cellular signaling pathway activity. The invention further relates to methods of measuring AR and / or TGFbeta pathway activity in a blood sample of a subject at risk of developing sepsis or suffering from sepsis, and administering an AR and / or a TGFbeta pathway inhibitor when the AR and / or the TGFbeta pathway activity exceeds a certain threshold.

Provided are a corn peptide having an anti-tumor effect, and a preparation method therefor and a use thereof. The corn peptide can obviously eliminate reactive oxygen species in tumor cells, increase the concentration of free calcium ions in the cytoplasm of the tumor cells and reduce the mitochondrial membrane potential of the tumor cells, and also can enhance the expression levels of mRNA and proteins of an IP3R and calreticulin, and further affect the calcium homeostasis of the tumor cells. In addition, the corn peptide can up-regulate the expression of tumor suppressor genes PTEN, P21 and P27, down-regulate the expression of a cell proliferation marker PCNA and a tumor-promoting gene Akt, and mediate an anti-tumor effect by means of PTEN and Akt cell signaling pathways.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a composition for assisting in protecting chemical liver injury and gastric mucosal injury as well as a preparation method and a product of the composition. The composition is prepared from the following components in parts by weight: 35 to 45 parts of radix puerariae extract, 25 to 35 parts of semen hoveniae extract, 25 to 35 parts of radix codonopsis extract, 28 to 33 parts of radix salviae miltiorrhizae extract and 25 to 35 parts of rhizoma atractylodis macrocephalae extract. The composition has a comprehensive treatment effect on alcoholic liver injury and gastric mucosal injury by synergistically regulating lipid metabolism, oxidative stress, inflammatory response and cell signal pathways. According to the technical scheme provided by the invention, comprehensive protection on alcoholic liver injury and gastric mucosal injury is realized through multi-channel cooperative regulation and control, and the defects of single target spot and limited curative effect of the existing therapeutic drug are overcome. The technical scheme provides a safe and effective intervention measure for digestive system injury caused by alcohol, and has a wide application prospect in the field of health-care product and medicine development.

The application provides a 1-heteroaryl-3-sulfone group bicyclo[1.1.1]pentane compound and a preparation method and application thereof, and belongs to the technical field of synthesis of pharmaceutical intermediates. The compound provided by the application is a double-substituted bicyclo[1.1.1]pentane containing a sulfone group and a heteroaryl group and derivatives thereof, wherein the sulfone group is an important pharmacophore, plays a role in antitumor drugs by participating in hydrogen bond interaction, regulating cell signaling pathways and the like, and the series of compounds have antitumor activity. The application uses cheap and easily available sulfonic acid chlorides, [1.1.1]spiro propeller and heteroaromatic compounds as raw materials, and prepares a series of sulfone group and heteroaryl group double-substituted bicyclo[1.1.1]pentane under mild conditions through a three-component tandem reaction with high selectivity and high yield, and the preparation method is simple and suitable for industrial production.

The present disclosure provides an antibody or an antigen-binding fragment thereof, wherein the antibody or the antigen-binding fragment thereof specifically binds to TSLP. The antibody of the present application can effectively inhibit the activation of TSLP on the STATs and JAK2 signaling pathway of H_TSLP Reporter Cell Line cells, and both the antibody of the present application and Tezepelumab can effectively inhibit the proliferation promoting effect of TSLP on BaF3-TSLPR / IL7Rα cells. The antibody of the present application does not cross the binding epitope of Tezepelumab, does not block the antibacterial peptide MKK34 with antibacterial activity at the C-terminus, and is expected to retain the ability of TSLP to resist bacterial infection from the outside.

The invention discloses a fungal broad-spectrum preservative resistance gene CDC7 and application thereof. The nucleotide sequence of the fungal preservative resistance gene CDC7 is at least one of the following (a), (b) and (c): (a) is as shown in SEQ ID NO: 1; (b) a variant sequence which has more than or equal to 95% sequence similarity with SEQ ID NO: 1 and encodes the same functional protein; and (c) a conservative modification sequence which is obtained by carrying out nucleotide substitution, deletion or increase on SEQ ID NO: 1 but does not change the coded amino acid sequence. The broad-spectrum preservative tolerance is endowed to fungi by regulating and controlling a cell signal channel. By constructing a recombinant vector containing the CDC7 gene and transforming the recombinant vector into a sensitive strain, the tolerance of the transgenic strain to preservatives such as potassium sorbate is remarkably enhanced. According to the invention, the sensitivity of the strain to preservatives can be improved, the CDC7 gene can be applied to other ascomycetes across species, and the defects of long period and low efficiency of traditional screening are overcome.

Methods and compositions for treating a subject suffering from adrenocortical carcinoma and having excess cortisol are disclosed. The methods provide therapeutic benefits including reduction of ACC tumor load, restoration of T-cell and natural killer (NK) cell signaling pathways, increase in T-cell and NK cell infiltration into the ACC tumor, reduction of neutrophil infiltration into the ACC tumor in the patient, and other therapeutic benefits. The methods include administration of a glucocorticoid receptor modulator (GRM) (which may be a selective glucocorticoid receptor modulator (SGRM)) and an antibody checkpoint inhibitor. In embodiments, the GRM (e.g., a SGRM) is orally administered. The GRM may be a nonsteroidal compound comprising: a fused azadecalin structure; a heteroaryl ketone fused azadecalin structure; or an octahydro fused azadecalin structure.

The present application discloses a chimeric antigen receptor molecule for specifically recognizing BAFF-R, a nucleic acid encoding the receptor molecule, an engineered immune effector cell comprising the receptor molecule, a pharmaceutical composition, and a use thereof. The receptor molecule comprises a structural domain that specifically recognizes the BAFF-R and an activation stimulation structural domain, and can activate a downstream signal path after specifically recognizing and binding the BAFF-R, thereby causing, promoting or improving an immune response reaction for the BAFF-R, and finally achieving the purpose of treating or preventing diseases caused by B cell BAFF-BAFF-R signal path imbalance.