Method for producing rice bran protein by enzyme hydrolysis method

A rice bran protein and enzymatic hydrolysis technology, which is applied in the separation, extraction and application fields of natural products, can solve the problems of rare rice bran protein powder and imperfect research content, and achieves improved resource utilization, good hot water instant solubility, and good quality. Effect

Inactive Publication Date: 2015-10-21
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the use of complex enzymes to assist in the extraction of rice bran protein and the pr

Method used

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  • Method for producing rice bran protein by enzyme hydrolysis method
  • Method for producing rice bran protein by enzyme hydrolysis method

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Embodiment 1

[0028] Vacuum-dry the fresh rice bran at 50°C for 4-5.5h, and crush it; take 500g of the rice bran raw material after crushing and degreasing (heat and reflux with n-hexane for 6-8h, remove the grease, evaporate the residual solvent after filtering), cellulase and starch Enzymes are mixed at a ratio of 1:3; the amount of mixed enzymes added is 1.5-3% of defatted rice bran, and the ratio of material to liquid is 1:6, the acid-base is adjusted to pH=5.5-6.5, and the enzymolysis temperature Control at 55°C, enzymatic hydrolysis for 2-3 hours, and magnetic stirring during the enzymatic hydrolysis process to maintain a stable pH (no change in pH); after the end of the enzymatic hydrolysis, there is no need to inactivate the enzyme, and immediately use sodium citrate with a concentration of 1-3mol / L to adjust Enzymolysis liquid to pH = 8.0-9.0, adjust the temperature 60-75 ℃, magnetic stirring and leaching for 3-4h; the mixture is centrifuged at 2000-3500r / min for 10-15min, discard t...

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Abstract

The invention provides a method for producing rice bran proteins by an enzyme hydrolysis method, and relates to a method for producing protein powder. Defatted rice bran is used as raw materials for extracting and preparing rice bran proteins. The method comprises the steps of raw material pretreatment, mixing enzymolysis, pH regulation, protein digestion, centrifugation, spraying drying and rice bran protein obtaining. The rice bran protein extraction rate reaches 76.19 percent, and the purity of the obtained rice bran proteins reaches higher than 70 percent. The method has the advantages that the extraction efficiency is high; the process is simple; the method is reliable; the stability is good; a path is provided for the integral utilization of rice bran resources and the preparation of the rice bran proteins.

Description

technical field [0001] The invention belongs to the field of separation, extraction and application of natural products, and in particular relates to a method for extracting rice bran protein from defatted rice bran and producing protein powder. Background technique [0002] Rice bran protein is a recognized high-quality vegetable protein, the content of which is 1 times higher than that of ordinary polished rice. The amino acid composition of rice bran protein is reasonable, close to the recommended mode of FAO / WHO, and its biological value is very high, which can be compared with milk and eggs. The lysine content reaches 5.8g / 100g protein, and the digestibility can reach more than 90%. It can be used as a functional ingredient of food. At the same time, rice bran protein is a hypoallergenic protein, which is suitable for special groups and nutritional food for infants and young children. [0003] The research on the extraction of rice bran protein began in the early 1970s....

Claims

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Application Information

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IPC IPC(8): A23J1/12A23J3/34
Inventor 丁之恩徐浩方洁杨松丁昱徐楠李菁楠
Owner ANHUI AGRICULTURAL UNIVERSITY
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