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Extraction method of auricularia auricula polysaccharide

A technology of fungus polysaccharides and extraction methods, which is applied in the field of extraction of natural active ingredients, can solve problems affecting the activity of fungus polysaccharides, and achieve the effects of simple and feasible extraction methods, improved utilization, good application value and market potential

Inactive Publication Date: 2018-03-30
金华市艾力生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This extraction method uses tannic acid solution to remove the protein in the filtrate, which will cause the denaturation of the polysaccharide of the fungus, and then affect the activity of the polysaccharide of the fungus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The extraction method of fungus polysaccharides includes ultrafine pulverization, enzymatic hydrolysis and wall breaking, microwave extraction, enzymatic hydrolysis-Sevage method for protein removal, and alcohol precipitation. The specific steps are:

[0016] 1) Superfine crushing: wash the fungus, dry, coarsely crush, add grinding aid to the fungus coarse powder at a weight ratio of 1000:0.011-0.013, and superfine crush it to get the fungus superfine powder. A mixture of tolbutamide and voglibose in a weight ratio of 1:0.33-0.38;

[0017] 2) Enzymatic wall breaking: add water to superfine fungus superfine powder according to the material-to-liquid ratio of 1:25-30, stir evenly, add yeast wall breaking enzyme according to the enzyme addition amount of 300-500U / g, and then add sorbitol buffer solution and β-mercaptoethanol, enzymolysis at 25-35°C for 70-90min, after enzymolysis, inactivate the enzyme at 90-100°C for 20-30min, then centrifuge at 4000-6000rpm for 14-18min ...

Embodiment 2

[0022] The extraction method of fungus polysaccharides includes ultrafine pulverization, enzymatic hydrolysis and wall breaking, microwave extraction, enzymatic hydrolysis-Sevage method for protein removal, and alcohol precipitation. The specific steps are:

[0023] 1) Superfine crushing: Wash the fungus, dry, coarsely crush, add grinding aid to the fungus coarse powder at a weight ratio of 1000:0.013, and superfine crush it to get the superfine fungus powder, which is used for future use, and the grinding aid is the weight ratio A 1:0.33 mixture of tolbutamide and voglibose;

[0024] 2) Enzymatic hydrolysis and wall breaking: Add water to superfine fungus superfine powder according to the material-to-liquid ratio of 1:30, stir evenly, add yeast wall breaking enzyme according to the enzyme addition amount of 500U / g, and then add sorbitol buffer and β- Mercaptoethanol, enzymolyzed at 25°C for 70 minutes, inactivated at 100°C for 20 minutes after the enzymolysis, and then centri...

Embodiment 3

[0029] The extraction method of fungus polysaccharides includes ultrafine pulverization, enzymatic hydrolysis and wall breaking, microwave extraction, enzymatic hydrolysis-Sevage method for protein removal, and alcohol precipitation. The specific steps are:

[0030] 1) Superfine crushing: Wash the fungus, dry, coarsely crush, add grinding aid to the fungus coarse powder at a weight ratio of 1000:0.012, and superfine crush it to get superfine fungus powder for use, the grinding aid is the weight ratio A 1:0.35 mixture of tolbutamide and voglibose;

[0031] 2) Enzymatic hydrolysis and wall breaking: add water to superfine powder of fungus fungus according to the material-to-liquid ratio of 1:27, stir evenly, add yeast wall-breaking enzyme according to the enzyme addition amount of 400U / g, and then add sorbitol buffer and β- Mercaptoethanol, enzymolyzed at 30°C for 80min, inactivated at 98°C for 25min after enzymolysis, and then centrifuged at 5000rpm for 16min to obtain fungus e...

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PUM

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Abstract

The invention discloses an extraction method of auricularia auricula polysaccharide. The extraction method comprises the following specific steps: carrying out superfine grinding on auricularia auricula to obtain auricularia auricula superfine powder; adding water into the auricularia auricula superfine powder, and adding yeast lysing enzymes to carry out enzymolysis and lysing, so as to obtain anauricularia auricula enzymatic hydrolysate and an auricularia auricula residue; adding water into the auricularia auricula residue, and carrying out microwave extraction to obtain an auricularia auricula extracting solution; combining the auricularia auricula enzymatic hydrolysate and the auricularia auricula extracting solution, carrying out filtering, adding protease for enzymolysis, carrying out centrifuging, and removing proteins of a supernate by using a Sevage method to obtain a deproteinized polysaccharide solution; and concentrating the deproteinized polysaccharide solution, then precipitating polysaccharide by using ethanol, and carrying out centrifuging, precipitating and freeze-drying to obtain the auricularia auricula polysaccharide. The extraction method provided by the invention is simple and feasible, the extraction rate and purity of the auricularia auricula polysaccharide are high, the auricularia auricula polysaccharide has good application values and market potential; and dissociative and zymolytic protein in a polysaccharide liquid can be effectively removed, so that the activity of the polysaccharide is kept from being damaged.

Description

technical field [0001] The invention relates to the field of extraction of natural active ingredients, in particular to a method for extracting polysaccharides from fungus. Background technique [0002] Auricularia auriculajudae (Auricularia auriculajudae), also known as Auricularia auriculajudae, is an edible and medicinal fungus of the fungus Basidiomycetes. Fungus is delicious and nutritious. It is not only an edible fungus with high nutritional value, but also a medicinal fungus with high medicinal value. It is a world-recognized health product. From the perspective of medicinal value, fungus has functions such as delaying aging, assisting in lowering blood fat, anticoagulation, anti-cancer, increasing immunity, assisting in lowering blood sugar, and anti-oxidation. A large number of studies have shown that the physiological functions of edible fungi are closely related to their polysaccharide components. Therefore In recent years, research on polysaccharides has also e...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 周艳初
Owner 金华市艾力生物科技有限公司
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