Evaluation method of internal protein nutrition based on LC-MS-MS technology

Abstract

The invention discloses an evaluation method of internal protein nutrition based on an LC-MS-MS technology. The evaluation method includes steps of (1), collecting different intestinal segment contents; extracting and separating protein components; (2), testing the protein concentration; (3), mass spectrum pretreatment including digestion and desalting of full protein; (4), LC-MS-MS analysis; (5),database searching; (6), data processing. The method applies a proteomic technology to identify the protein and its digested product in different intestinal segment contents; on this basis, source ofthe proteins of the intestinal segment contents and the contents can be judged; through the bioinformatics analysis, the function of different proteins at the inner part of an organ can be learned deeply, wherein the gene expression of enzyme related to protein digestion and metabolism may be different; the method provides a scientific basis for scientific evaluation of the protein digestion anduse.

Description

technical field [0001] The invention belongs to the technical field of proteomics, and relates to a method for evaluating protein nutrition in vivo based on LC-MS-MS technology. Background technique [0002] The more authoritative method for measuring protein digestibility in vivo is fecal nitrogen balance test. The principle is that dietary protein enters the gastrointestinal tract through the esophagus, pepsin in the stomach digests dietary protein, polypeptides enter the small intestine (duodenum, jejunum, and ileum) and are acted on by trypsin and chymotrypsin. Finally, a small amount of microorganisms When cells, fecal metabolized nitrogen shed from intestinal mucosa and incompletely digested and absorbed protein pass through the colon, they are excreted in the form of feces. The ratio of the difference between intake nitrogen and fecal nitrogen to intake nitrogen is the apparent digestibility. Although 5% highly digestible casein will be added and fecal metabolic nit...

AI Technical Summary

Problems solved by technology

Although adding 5% highly digestible casein and then measuring fecal metabolic nitrogen to correct the apparent digestibility to obtain the true digestibility, this method cannot faithfully reflect the protein digestion and absorption in the body

This in vivo digestibility determination method is complicated to operate and takes a long time, and the experimental animals have high requirements on the external environment.

The traditional method only knows about nitrogen intake and nitrogen excretion, lacks an in-depth understanding of the internal digestion, absorption and metabolism of the body, and cannot truthfully reflect the protein digestion in the body

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