Method for extracting fishskin collagen polypeptide from fish wastes

A technology of collagen polypeptide and waste, which is applied in the field of extracting fish skin collagen polypeptide from fish waste, can solve the problems of high cost of pet food, and achieve the effects of high utilization rate, convenient operation and simple process

Inactive Publication Date: 2009-04-01
QINGDAO BETTER BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the cost of pet foo

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Example 1: Wash and drain the fish skin first, then add 8 times demineralized water (w / w), adjust the pH to 4.7, stir and extract at 92°C for 4 hours, coarse filter; adjust the pH to 8.3, add kaolin to 65 Fine filtration at ℃, the fine filtrate is single-helix fish skin collagen; when the fine filtrate is concentrated to 1 / 3 under reduced pressure, add n-hexane or Petroleum ether or diethyl ether, stir for 1 hour, stand and extract for 6-8 hours; adjust the pH of the lower layer solution to 6.8, and adjust the temperature to 51-53°C; according to fresh fish skin: high-efficiency enzyme mixture = 1:0.002 (W / W) The ratio of high-efficiency enzyme mixture is added to the one-step enzymatic digestion for 3-5 hours, and at the same time, it is continuously stirred; Stir and decolorize at 65°C for 0.5 hours, filter, desalt the filtrate through a nanofiltration membrane, concentrate in a vacuum, and spray dry to obtain colorless, odorless, fat-free, low-salt and uniform molecu...

Example Embodiment

[0016] Embodiment 2: the fish fillets are first separated from the fish meat with a bone and meat separator, and then demineralized water is added to the fish meat, and the temperature is adjusted to 50 ° C. According to fresh fish meat: neutral protease = 1: 0.002 (w / w), add papain, and keep stirring Enzymolysis was carried out for 3 hours; the enzymolysis solution was deodorized and spray-dried to obtain fish meat hydrolyzed protein powder; the fish bones were transferred to fish heads and fish viscera for treatment, which was Example 3.

Example Embodiment

[0017] Example 3: Dry the fish head, fish viscera together with the fish bones separated in Example 2 at 95-100°C, crush them, and pass through a 40-mesh sieve to obtain the main raw material of pet food, which is used to compound into pet food .

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PUM

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Abstract

The invention relates to a method for extracting fishskin collagen polypeptide by taking the wastes of such fishes as codfish, salmon or tilapia and the like as the raw materials, which comprises the processing steps of extracting single-spiral collagen, removing fishy smell and deodorizing, enzyme cutting, decolourization, desalting and spray drying; the fishskin containing natural collagen of triplehelix tight structure is washed, drained and then added into softened water for being extracted for three hours by controlling the pH value and the temperature condition, then the pH value is adjusted again and diatomite is added for coarse filtration, and the coarse filtrate is the single-spiral fishskin collagen; and the coarse filtrate is carried out decompress concentration and added with organic solvent for being mixed and stirred for 1 hour, and then rest, extracted and carried out fishy smell removing and deodorizing; and then an enzyme mixture is added to enzyme-cut the solution and a decoloring agent is also added for decoloring for 0.5 hour when the temperature control and stirring are carried out, the solution is then filtered; and finally, desalting and spray drying are carried out to obtain white fishskin collagen polypeptide powder products. The method has simple technical process, convenient operation and control, high utility ratio and good quality.

Description

Technical field: [0001] The invention relates to a method of extracting fish skin collagen polypeptide from fish waste, which utilizes fish waste such as cod, salmon or tilapia as raw materials, and undergoes high-value and clean process steps to realize comprehensive utilization of fish processing waste. Methods. Background technique: [0002] my country is rich in aquatic product resources, accounting for 36% of the world's total aquatic products. There are more than 7,000 aquatic product processing enterprises in China, but most of them are only primary processing. About 57% of the waste is left after the fish is exported, and the waste is simply used as feed or fertilizer, but lacks comprehensive utilization. However, there is growing interest in deriving high-value health-enhancing products from seafood. The aquaculture processing industry produces a large amount of marine fish wastes such as fish bones, skins, fins and scales every year. Although these wastes have hi...

Claims

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Application Information

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IPC IPC(8): C12P21/08C07K1/14
Inventor 魏玉西曹荣军
Owner QINGDAO BETTER BIO TECH
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