9 results about "Amino acid binding" patented technology

Obtained is: a method for efficiently producing a polypeptide containing a non-canonical amino acid; a method for incorporating a non-canonical amino acid into a polypeptide; a method for producing tRNA bound to a non-canonical amino acid; a non-canonical amino acid incorporation system; a material for use in these methods; or the like. Used is a method for producing a polypeptide containing a non-canonical amino acid, comprising: a step of contacting PylRS from an organism belonging to the order Methanomassiliicoccales or Thermoplasmatales with the non-canonical amino acid; and an incorporation step selected from step (a) of incorporating the non-canonical amino acid into the polypeptide with higher efficiency than in a case of using a cell-free protein synthesis system with PylRS of Methanosarcina mazei (MmPylRS) or step (b) of incorporating the non-canonical amino acid into the polypeptide with higher efficiency than in a case of using an Escherichia coli protein synthesis system with a vector carrying a gene for the PylRS under regulation by a glmS promoter or a case of using an Escherichia coli protein synthesis system with a vector carrying a gene for the PylRS under regulation by a glnS promoter. Alternatively, used is a non-canonical amino acid incorporation system containing highly concentrated PylRS from an organism belonging to the order Methanomassiliicoccales or Thermoplasmatales.

The present invention relates to a pharmaceutical composition for preventing or treating muscle diseases. Specifically, the elderberry extract or monosaccharide and amino acid combined compound disclosed by the invention inhibits apoptosis of muscle cells, inhibits expression of inflammatory cytokines, promotes secretion of testosterone and inhibits expression of amyotrophy marker protein and myogenesis inhibition protein, and shows an effect of recovering muscle in a sarcopenia animal model; therefore, the device can be effectively used in the treatment of muscle diseases.

The present invention provides methods for identifying and sequencing proteins, polypeptides, and amino acids, as well as compositions useful for them. [Solution] The present invention provides amino acid recognition molecules, such as amino acid-binding proteins and their fusion polypeptides, which differentially associate with different types of amino acids to generate detectable characteristic signatures that serve as indicators of the amino acid sequence of polypeptides. The present invention also provides amino acid recognition molecules that include a shielding element to enhance photostability during polypeptide sequencing reactions.

Provided herein are binding agents that selectively bind to individual monomer types (e.g., amino acid types) of a polymeric analyte (e.g., a peptide). The present disclosure provides methods for producing and, optionally, engineering binding agents. Such binding agents can be used for a variety of applications, including single-molecule peptide or protein sequencing.

There is provided a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) at least one sumoylated lysine residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said sumoylated lysine residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs. Also provided is a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) a C-terminal aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said C-terminal aspartic acid residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs.

Provided herein are novel amino acid binding proteins that recognize aspartate and / or glutamate in protein sequencing reactions; novel amino acid binding proteins that recognize arginine in protein sequencing reactions; and novel amino acid binding proteins that recognize glycine, alanine, and / or serine in protein sequencing reactions.

Provided herein are novel amino acid binding proteins that recognize aspartate and / or glutamate in protein sequencing reactions; novel amino acid binding proteins that recognize arginine in protein sequencing reactions; and novel amino acid binding proteins that recognize glycine, alanine, and / or serine in protein sequencing reactions.

The invention discloses an application of ganoderic acid A as an intestinal farnesic acid X receptor (FXR) inhibitor in preparation of a product for preventing and / or treating hyperlipidemia. Molecular docking software is used for predicting that ganoderic acid A can be combined with FXR and occupies an active site of the FXR, and a possible amino acid binding site is a TYR (tyrosine) 369 amino acid residue; the application of a Caco2 cell model proves that the ganoderic acid A can penetrate through a cell membrane to be directly combined with the FXR in the cell; a surface plasmon resonance result proves that ganoderic acid A can competitively inhibit the combination of an FXR endogenous agonist and FXR so as to inhibit the activity of the FXR endogenous agonist. By inhibiting the activity of FXR, the ganoderic acid A can significantly inhibit the expression of intestinal FXR target gene ileum bile acid binding protein, fibroblast growth factor 15 and small heterodimer partner, and effectively improve C57BL / 6J mouse hyperlipidemia induced by high fat diet.

The invention relates to the field of developing agents, in particular to a fingerprint developing agent and a developing method and application thereof. Wherein the fingerprint developing agent comprises a compound I and an organic solvent; the molecular structural formula of the compound I is I; wherein X is selected from CH3O <->,-CH3,-CH2CH3 or NO2; y is selected from CH3O <->,-CH3,-CH2CH3, NO2 or halogen; x and Y are the same or different. Compared with a traditional fingerprint display method, the compound can realize higher sensitivity and clearer imaging effect; and only protein and amino acid in fingerprints are combined to form a fluorescent product, and other substances (such as paper and the like) do not have a showing effect, so that highly selective fingerprint showing can be realized. And the method has a good application value in the actual development of the developing reagent.