Method for preparing ACE inhibition peptide originate from fish skin

A technology of inhibitory peptides and fish skins, which is applied in the field of preparation of ACE inhibitory peptides derived from fish skins, can solve the problems of lack of depth, environmental pollution, unsystematic, etc., and achieve the effect of scientific and reasonable process, high safety and low cost

Inactive Publication Date: 2008-08-13
NANCHANG UNIV
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Problems solved by technology

If these leftovers are not used effectively, it will not only cause great waste, but also cause environmental pollution.
At present, the research on fish collagen in foreign count

Method used

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  • Method for preparing ACE inhibition peptide originate from fish skin

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Example Embodiment

[0031] Implementation case:

[0032] Weigh 100.0008g of clean tilapia skins, immerse them in 700ml of 0.1mol / L hydrochloric acid solution and 0.1mol / L sodium hydroxide solution for 1hr after degreasing treatment, and wash with distilled water to neutrality, at 45℃. Homogenize with 1000ml of distilled water for 12 hours, extract the supernatant by suction filtration, heat to 50°C-55°C, adjust the pH to 6.0, add 0.04671g papain for constant temperature enzymatic hydrolysis for 2 hours, and then in a water bath at a high temperature of 100°C After heating for 20 minutes, the enzymatic hydrolysis reaction is terminated, and the fish skin proteolysis solution is obtained. The hydrolysis degree of tilapia fish skin protein is 12.3%.

[0033] A compound decolorant (mixed with 3g / L of activated carbon yeast and cyclodextrin) is used to debitter, decolor and deodorize the fish skin proteolysis solution, and collect the supernatant by centrifugal filtration at 4000 r / min. Pass 001*7(732) ca...

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Abstract

A method for preparing ACE inhibition peptides from fish skin is provided. The method is prepared with tilapia skin, extracts fish skin albumen from tilapia skin, adapts prolease to hydrolysis through enzyme extinguishing, debitterizing, decolouring, de-flavoring, filtering, ion exchanging and concentrating to obtain fish skin proteolysis liquor, after untruly filter (cutoff molecular mass is 9000Da) the TSP-I, collect high activity component by SephadexG-15 gel chromatography, freeze and dry to obtain high activity ACE inhibition peptides. The invention has important meaning for satisfying integrated utilization of tilapia skin wastes and improving its attachment.

Description

technical field [0001] The invention relates to a preparation method of ACE inhibitory peptide derived from fish skin. Background technique [0002] Angiotensin-converting enzyme (ACE) plays an important role in the regulation of blood pressure. In KKS), ACE uses angiotensin I and bradykinin as substrates, causing vasoconstriction and causing blood pressure to increase. [0003] Angiotensin-converting enzyme inhibitors (ACEI) are the main effective drugs for the treatment of hypertension and heart failure today. The chemically synthesized ACE inhibitors have exposed many defects in clinical use, such as: short action time, easy blood pressure rebound, and harmful to human body. Damage to multiple parts of the body, etc. Angiotensin-converting enzyme inhibitory peptide, an ACE inhibitor derived from food protein, has attracted widespread attention because of its high food safety and no side effects. [0004] At present, antihypertensive peptides with ACE inhibitory activit...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 刘成梅梁汉萦刘伟梁瑞红涂宗财
Owner NANCHANG UNIV
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