Method for preparing ACE inhibition peptide originate from fish skin

A technology of inhibitory peptides and fish skins, which is applied in the field of preparation of ACE inhibitory peptides derived from fish skins, can solve the problems of lack of depth, environmental pollution, unsystematic, etc., and achieve the effect of scientific and reasonable process, high safety and low cost

Inactive Publication Date: 2008-08-13
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AI-Extracted Technical Summary

Problems solved by technology

If these leftovers are not used effectively, it will not only cause great waste, but also cause environmental pollution.
At present, the research on fish collagen in foreign count...
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A method for preparing ACE inhibition peptides from fish skin is provided. The method is prepared with tilapia skin, extracts fish skin albumen from tilapia skin, adapts prolease to hydrolysis through enzyme extinguishing, debitterizing, decolouring, de-flavoring, filtering, ion exchanging and concentrating to obtain fish skin proteolysis liquor, after untruly filter (cutoff molecular mass is 9000Da) the TSP-I, collect high activity component by SephadexG-15 gel chromatography, freeze and dry to obtain high activity ACE inhibition peptides. The invention has important meaning for satisfying integrated utilization of tilapia skin wastes and improving its attachment.

Application Domain

Peptide preparation methodsFermentation

Technology Topic

IonIon exchange +8


  • Method for preparing ACE inhibition peptide originate from fish skin


  • Experimental program(1)

Example Embodiment

[0031] Implementation case:
[0032] Weigh 100.0008g of clean tilapia skins, immerse them in 700ml of 0.1mol/L hydrochloric acid solution and 0.1mol/L sodium hydroxide solution for 1hr after degreasing treatment, and wash with distilled water to neutrality, at 45℃. Homogenize with 1000ml of distilled water for 12 hours, extract the supernatant by suction filtration, heat to 50°C-55°C, adjust the pH to 6.0, add 0.04671g papain for constant temperature enzymatic hydrolysis for 2 hours, and then in a water bath at a high temperature of 100°C After heating for 20 minutes, the enzymatic hydrolysis reaction is terminated, and the fish skin proteolysis solution is obtained. The hydrolysis degree of tilapia fish skin protein is 12.3%.
[0033] A compound decolorant (mixed with 3g/L of activated carbon yeast and cyclodextrin) is used to debitter, decolor and deodorize the fish skin proteolysis solution, and collect the supernatant by centrifugal filtration at 4000 r/min. Pass 001*7(732) cation exchange resin at room temperature at a flow rate of 8 times the column volume/h, collect the effluent, and measure its nitrogen recovery rate of 95.40%. Then the decationized hydrolysate is 8 times the column volume/ The flow rate of h passes through 201*7 (717) anion exchange resin, and the effluent is collected. The nitrogen recovery rate is 93.31% and the salt rejection rate is 93.15%. After the effluent is concentrated, it is subjected to ultrafiltration treatment. The working pressure of the ultrafiltration equipment is 28 psi, the temperature of the material liquid is 29°C, the pH of the material liquid is 6.7, and the ultrafiltration treatment is 40 minutes to obtain the fish skin polypeptide liquid.
[0034] The fish skin polypeptide solution was separated by gel chromatography, the separation medium was Sephadex G-15, and the chromatographic column size was 1.6×60cm. Column chromatography conditions: sample amount: 1.5ml; flow rate: 0.8ml/min; eluent: distilled water; detection wavelength: 220nm. Under this separation condition, 8 components are obtained. The high-activity ACE-inhibiting component F is pre-frozen at -80°C for 8 hours and freeze-dried in vacuum for 72 hours to obtain the product. The half inhibitory concentration of each component is shown in Table 2.
[0035] Table 2 The half inhibitory concentration (IC 50 )
[0036] Component


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Description & Claims & Application Information

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