Complex of marine oligosaccharides and collagen peptides, preparation method thereof and application thereof

A collagen peptide and compound technology is applied in the field of the compound of marine characteristic oligosaccharide and collagen peptide to achieve important economic and social benefits and strong antioxidant effects

Inactive Publication Date: 2011-10-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the complexation between low molecular weight mannuronic acid and collagen peptides and its effect on antioxidant activity.

Method used

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  • Complex of marine oligosaccharides and collagen peptides, preparation method thereof and application thereof
  • Complex of marine oligosaccharides and collagen peptides, preparation method thereof and application thereof
  • Complex of marine oligosaccharides and collagen peptides, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, preparation method

[0041] 1. Preparation of mannuronic acid with antioxidant activity and a degree of polymerization of about 2-8

[0042]Use the HCL solution with a concentration of 0.1-0.3M to make a suspension solution with a weight percent concentration of 1-10% by β-D-(1,4)-connected polymannuronic acid (average molecular weight 7000Da), and then, Hydrolyze in a high-pressure reactor at 120°C for 1 hour, adjust to pH 7 with 0.1% sodium hydroxide after cooling, filter through a 0.45 μm membrane, concentrate by rotary evaporation, separate with a Bio-Gel-P6 column, and use 0.1M ammonium bicarbonate The buffer solution is used for elution to obtain oligosaccharides with a degree of polymerization of 2-8, which are desalted with Sephadex G10 and freeze-dried to obtain a brown powder, which is the mannuronic acid product required by the present invention.

[0043] 2. Preparation of tilapia collagen peptide with antioxidant activity and molecular weight ...

Embodiment 2

[0048] Embodiment 2, oxygen free radical scavenging experiment

[0049] The purpose of the experiment: to determine the effect of the compound of mannuronic acid and collagen peptide on the oxygen free radical DPPH·, O at the molecular level 2 - ·As well as the scavenging ability of OH., and compare the antioxidant effect of the compound with its single component, in order to determine the effect of the compound on the improvement of the antioxidant activity of its single component.

[0050] Experimental method: Weigh 20 mg of the compound, tilapia fish skin collagen peptide with a molecular weight of 2800-3000 Da and mannuronic acid with a polymerization degree of 2-8, and prepare a sample solution of 0.25-20 mg / ml. The oscillator is fully oscillating and ready to be tested. Using DPPH method, AP-TEMED spectrophotometry and o-phenanthroline-Fe 2+ The antioxidant activity of the complex and its single components was detected by the oxidation method.

[0051] Experimental r...

Embodiment 3

[0053] Embodiment 3, MTT cell viability experiment

[0054] Purpose of the experiment: The protective effect of the compound of mannuronic acid and collagen peptide on skin cells damaged by ultraviolet rays was detected by MTT method at the cellular level, and the antioxidant effect of the compound and its single component was compared in order to determine Compounding can improve the anti-ultraviolet activity of its single component.

[0055] Experimental method: Fetal mouse fibroblasts (MEF) and human immortalized keratinocytes (HaCaT) were randomly divided into control group (normal culture cells), model group (ultraviolet irradiation group) and drug-dosing group (different sample treatment + ultraviolet irradiation group) irradiation). When the cell growth was close to 80% confluence, under sterile conditions, the final concentration of 10, 20, 50, 100, 200, 500 and 1000 μg / ml of the complex, tilapia fish skin collagen peptide was measured by the degree of polymerization ...

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Abstract

The invention provides an ultraviolet radiation damage resistant complex of marine oligosaccharides and collagen peptides, which is prepared from marine oligosaccharides with antioxidant activity and tilapia mossambica fishskin collagen peptides with molecular weight of between 2,800 and 3,000 Da through compatibility. Chemical and pharmacological experiments prove that: compared with single components subjected to compatibility, the complex can inhibit the generation of oxygen radicals caused by ultraviolet radiation obviously and can enter skin cell mitochondria in a targeting mode to a greater degree to inhibit light aging, and has the obvious protective effect on skin injury caused by the ultraviolet radiation. The complex provides theoretic support for the development of natural oligosaccharide-peptide complex uvioresistant and mitochondrion targeting medicaments and the high-value utilization of mariculture industry.

Description

technical field [0001] The invention relates to a complex of marine characteristic oligosaccharides and collagen peptides, a preparation method and application thereof. Background technique [0002] In recent years, with the emergence of the ozone hole, excess oxygen free radicals caused by ultraviolet radiation have seriously endangered human health and the environment. The skin is the organ of the human body that receives the most direct and largest area of ​​ultraviolet radiation. As the first natural protective barrier of the body, the damage caused by excessive oxygen free radicals produced by ultraviolet radiation is the most obvious. Studies have shown that photoaging and cell apoptosis and necrosis, which occur in the initial process of photodamage, have a strong role in promoting the formation of skin tumors. Therefore, skin diseases induced by ultraviolet rays have attracted people's great attention. [0003] In order to slow down the photodamage caused by ultrav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/39A61K8/73A61K8/65A61Q19/00A61P17/18A61P39/06A61K31/731A61K31/722A61K31/715A61K31/702A61K8/60
Inventor 管华诗任舒文
Owner OCEAN UNIV OF CHINA
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