Application of 32 soybean InDel markers in detection of soybean genetic diversity

A genetic diversity and soybean technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of non-standard management of the seed industry market, homogeneous names, soybean seeds with the same name and different species, etc.

Active Publication Date: 2021-10-12
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there are certain problems in the development of my country's soybean seed

Method used

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  • Application of 32 soybean InDel markers in detection of soybean genetic diversity
  • Application of 32 soybean InDel markers in detection of soybean genetic diversity
  • Application of 32 soybean InDel markers in detection of soybean genetic diversity

Examples

Experimental program
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Embodiment 1

[0099] Example 1, Application of Soybean Marker Combination in Detecting Soybean Genetic Diversity

[0100] This example provides a soybean InDel marker combination consisting of 32 insertion / deletion markers (InDel markers), which can reflect the genetic diversity of 96 soybean materials.

[0101] 1. Discovery of soybean InDel markers

[0102] The inventors of the present invention used Illumia HiSeqTM2500 to carry out whole-genome resequencing of 12 soybean materials: extract plant material DNA and use ultrasound to fragment DNA of qualified plant material, and then perform fragment purification, end repair, and 3 Add A at the 'end, connect the sequencing linker, select the fragment length and size by agarose gel electrophoresis, establish a sequencing library by PCR amplification, and use Illumina HiSeqTM 2500 to sequence the qualified library. The quality of the original reads obtained by sequencing is evaluated, and the Clean reads are obtained by filtering, which are us...

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Abstract

The invention discloses an application of 32 soybean InDel markers in detection of soybean genetic diversity. The 32 soybean InDel markers are 32 DNA molecules obtained by performing PCR amplification on soybeans by using 32 pairs of primers as shown in sequences 1-64 in a sequence table. The 32 soybean InDel markers can be used for detecting the soybean genetic diversity, identifying soybean varieties or strains or developing molecular markers for identifying the soybean varieties or strains, preparing soybean genetic maps, analyzing soybean genetic structures and further breeding soybeans. The 32 soybean InDel markers and the complete set of primers for detecting the 32 soybean InDel markers have wide research prospects.

Description

technical field [0001] The invention relates to the application of 32 soybean InDel markers in the detection of soybean genetic diversity in the field of biotechnology. Background technique [0002] Soybean (Glycine max (L.) Merrill) originated in China and is a dicotyledonous herbaceous plant evolved from wild species cultivation. It belongs to the family Leguminsae, Papiliontae, and Glycine. Soybean provides 1 / 3 of the sources of vegetable protein and vegetable oil for humans, and is one of the five major crops in my country. Soybean is a basic, economical, and strategic crop related to people's livelihood. Its industry has great development potential and plays a pivotal role in the trade of agricultural products. In recent years, with the adjustment of the supply-side reform of the Ministry of Agriculture, the soybean seed industry system has been diversified. my country's soybean seed industry market is very active, and a relatively complete soybean breeding system has...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 闫龙李曼张洁杨春燕邸锐史晓蕾刘兵强娜仁赵青松秦君王冬梅张孟臣
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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