Polymorphic primers of pheobe chekiangensis nuclear genome SSR molecular marker and application of polymorphic primers

A technology of polymorphic primers and molecular markers, applied in the fields of application, plant gene improvement, climate sustainability, etc., can solve the problems that are difficult to comprehensively reveal the genetic diversity of Phoebe chinensis, and achieve wide coverage and high resolution Effect

Active Publication Date: 2021-12-17
JIANGXI ACAD OF FORESTRY
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Problems solved by technology

In the existing research, only the populations in local areas, that is, the 6 populations in Zhejiang and Jiangxi, it is difficult to fully reveal the diversity of the genetic level of Zhejiang Nan.

Method used

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  • Polymorphic primers of pheobe chekiangensis nuclear genome SSR molecular marker and application of polymorphic primers
  • Polymorphic primers of pheobe chekiangensis nuclear genome SSR molecular marker and application of polymorphic primers
  • Polymorphic primers of pheobe chekiangensis nuclear genome SSR molecular marker and application of polymorphic primers

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Experimental program
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Effect test

Embodiment 1

[0057]1. Test materials

[0058] The test materials come from 10 populations in the natural distribution area of ​​Zhejiang Nan, namely Dexing in Jiangxi, Wuyuan in Jiangxi, Shicheng in Jiangxi, Lushan in Jiangxi, Hangzhou in Zhejiang, Ningbo in Zhejiang, Songyang in Zhejiang, Wuhan in Hubei, Qimen in Anhui, and Wugang in Hunan. . Select the ancient Zhejiang Nan, collect 8-10 mature leaves from each individual, and store them dry on silica gel. The sample size for each group is shown in Table 1.

[0059] Table 1 Sampling information of 10 Zhejiang Nan populations

[0060] group sample size source JX_DX 15 Jiangxi Dexing JX_WY 30 Wuyuan, Jiangxi JX_SC 30 Shicheng, Jiangxi JX_LS 7 Mount Lu, Jiangxi ZJ_HZ 12 Hangzhou, Zhejiang ZJ_NB 11 Ningbo, Zhejiang ZJ_SY 10 Zhejiang Songyang HB_WH 15 Wuhan, Hubei AH_QM 30 Anhui Qimen HN_WG 15 Wugang, Hunan

[0061] 2. Extraction and detection ...

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Abstract

The invention discloses polymorphic primers of a pheobe chekiangensis nuclear genome SSR molecular marker and an application of the polymorphic primers, and belongs to the technical field of forestry molecular biology. 15 pairs of polymorphic primers of the pheobe chekiangensis nuclear genome SSR molecular marker are developed through a PCR technology, a polyacrylamide gel electrophoresis technology and a capillary electrophoresis technology, and the nucleotide sequences of the primers are as shown in SEQ ID NO.1 to SEQ ID NO.30. The polymorphic primers of the pheobe chekiangensis nuclear genome SSR molecular marker are good in specificity and high in polymorphism; and when the polymorphic primers are applied to analysis of the genetic structure and resource genetic diversity of the pheobe chekiangensis, the diversity and the genetic structure of the genetic level of the pheobe chekiangensis in China can be disclosed more comprehensively, and a foundation is laid for formulating a genetic protection strategy.

Description

technical field [0001] The invention belongs to the technical field of forestry molecular biology, and in particular relates to polymorphic primers of SSR molecular markers in the Zhejiang Phoebe genome and its application in the analysis of the genetic structure and genetic diversity of the Zhejiang Phoebe population. Background technique [0002] Zhejiang Nan (Pheobe chekiangensis C.B.Shang), Zhejiang Nan (Phoebe chekiangensis C.B.Shahg) is a plant of Lauraceae (Lauraceae) Phobe (Phobe), is a famous Chinese plant taxonomist Professor Xiang Qibo in the 1960s in Zhejiang A new species discovered in Tianmu Mountain and Longtang Mountain. Zhejiang Nan is mainly distributed in Zhejiang, Jiangxi, Anhui, Fujian and other places in southern my country. Zhejiang Phoebe is scattered as an associated species in the natural evergreen broad-leaved forests of Fagaceae species in the mid-subtropical region of my country. Due to long-term human destruction, only in the deep mountains and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11A01H1/04
CPCC12Q1/6895C12Q1/6858A01H1/04C12Q2600/156C12Q2531/113C12Q2565/125Y02A90/40
Inventor 郑永杰伍艳芳刘新亮张月婷周诚温世钫徐海宁杨海宽郭捷
Owner JIANGXI ACAD OF FORESTRY
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