Polymorphic primers and applications of ssr molecular markers in camphor nuclei genome

A polymorphic primer, nuclear genome technology, applied in recombinant DNA technology, biochemical equipment and methods, microbial assay/inspection, etc., can solve the problem of low polymorphism, poor repeatability, and difficulty in fully revealing the genetic diversity of camphor Real level and other issues, to achieve the effect of wide coverage and good repeatability
CN110724760BActive Publication Date: 2021-03-19INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
Publication Date
2021-03-19

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Abstract

The invention discloses a polymorphic primer of a cinnamomum camphora nuclear genome SSR molecular marker and application thereof, and belongs to the technical field of forestry molecular biology. Thenucleotide sequence of the primer is shown as SEQ ID NO.1 to SEQ ID NO.14. The polymorphic primer is applied to the application of variety identification of cinnamomum camphora, and genetic structureand resource genetic diversity analysis of ancient cinnamomum camphora groups. A method includes the steps of genome DNA extraction, PCR amplification, polyacrylamide gel electrophoresis and data analysis. 186 individuals of 18 ancient cinnamomum camphora groups are subjected to genetic structure and resource genetic diversity study; the possibly forming reasons are analyzed according to existinggenetic structure and resource genetic diversity patterns of the cinnamomum camphora; and theoretical basis is provided for the wild cinnamomum camphora resource protection and utilization. The repeatability of the SSR molecular marker is high, and the real level of the genetic diversity of the cinnamomum camphora can be comprehensively disclosed.
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Description

technical field

[0001] The invention belongs to the technical field of forestry molecular biology, and in particular relates to polymorphic primers for SSR molecular markers of Cinnamomum camphora nuclei genome and applications thereof. Background technique

[0002] Cinnamomum camphora (L.) Presl is a representative species of subtropical evergreen broad-leaved forest, widely distributed in the south of the Yangtze River in my country. Cinnamomum camphora has a long history of cultivation and utilization in my country, and the resources of ancient camphor are rich, containing valuable genetic resources, but there are few reports on the level of genetic variation and its phylogenetic relationship.

[0003] Based on the research results of DNA molecular markers, the conclusions are different. Yao Xiaohua used 9 pairs of RAPD primers to analyze the genetic diversity of 82 families of Cinnamomum camphora from 10 provenances. A total of 146 polymorphic bands were amplified. The ...

Claims

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