Polymorphic primers and applications of SNP molecular markers in Cinnamomum camphora nuclear genome

Abstract

The invention discloses a polymorphism primer of a yellow cinnamon root or stem nuclear genome SNP molecular marker and an application of the polymorphism primer, and belongs to the technical field offorestry molecular biology. The primer nucleotide sequence of the polymorphism primer comprising 12 pairs of yellow cinnamon root or stem genome SNP molecular markers is shown as SEQID NO.1-SEQ NO.24, and the polymorphism primer is applied to identification of yellow cinnamon root or stem varieties, ancient tree park genetic structures and resource heredity diversity analysis. Through extractionof different varieties of yellow cinnamon roots or stems, PCR amplification, HRM analysis and data gathering, 186 individuals of the 18 yellow cinnamon root or stem groups are subjected to heredity structure and heredity diversity research, and according to the existing heredity structure and the heredity diversity pattern of the yellow cinnamon roots or stems, the reason for possibility formationis analyzed. A theory basis is provided for utilization of wild yellow cinnamon root or stem resource protection. The SNP molecular marker is good in repeatability, and can comprehensively reveal true level of the heredity diversity of the yellow cinnamon roots or stems.

Description

technical field [0001] The invention belongs to the technical field of forestry molecular biology, and in particular relates to polymorphic primers for SNP molecular markers of Cinnamomum camphora nuclei genome and applications thereof. Background technique [0002] Camphor is a representative species of subtropical evergreen broad-leaved forest in eastern Asia, and is widely distributed in the south of the Yangtze River in my country. Cinnamomum camphora has a wide range of uses, and the material of camphor wood is excellent. Together with Nan, Catalpa and Tong, it is called the "Four Famous Woods in the South of the Yangtze River". In addition, camphor oil extract is also an important chemical raw material, widely used in medicine, cosmetics and food processing industries. The cultivation of camphor has a long history, and there are records of the cultivation and utilization of camphor as early as the Spring and Autumn Period of the Eastern Zhou Dynasty. In recent years, ...

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Problems solved by technology

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide polymorphic primers for the SNP molecular markers of the camphor nuclear genome to solve the problem of poor repeatability and low polymorphism in the prior art using co-dominant DNA markers such as RAPD and ISSR. Another object of the present invention is to provide the application of polymorphic primers of Cinnamomum camphora nuclear genome SNP molecular markers in Cinnamomum camphora species identification, genetic structure and resource genetic diversity analysis, so as to solve the problems existing in the prior art that are difficult to be comprehensive The problem of revealing the true level of Cinnamomum camphora genetic diversity

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