Narcissus BES-SSR marker primer GA19 and method for identifying narcissus species

A technology for labeling primers and narcissus, which is applied in the field of molecular biology, can solve the problems of low polymorphism of SSR markers and insufficient development of SSR markers, and achieves effective technical support, effective and reliable results, and good repeatability.

Inactive Publication Date: 2014-02-05
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0006] At present, there are mainly two types of SSR markers developed: G-SSR (genome SSR) and EST-SSR (expressed sequence tag SSR), and the existing Narcissus EST database There are still very few narcissus EST sequences registered in China, and the development of SSR markers...

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  • Narcissus BES-SSR marker primer GA19 and method for identifying narcissus species
  • Narcissus BES-SSR marker primer GA19 and method for identifying narcissus species

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Embodiment 1

[0024] Example 1 A method for identifying narcissus species using the narcissus BES-SSR marker primer GA19

[0025] A method for identifying narcissus varieties using narcissus BES-SSR labeled primer GA19, including the design and synthesis of narcissus BES-SSR labeled primer GA19, the extraction of total DNA from the narcissus genome, the establishment of a total PCR reaction system, PCR amplification procedures, and polyacrylamide gel electrophoresis conditions and Narcissus species identification.

[0026] 1. Design and synthesis of Narcissus BES-SSR labeled primer GA19: According to the search results of Narcissus BES-SSR loci, design and synthesize Narcissus GA19 BES-SSR labeled primers as follows:

[0027] GA19 F: 5'-GAGAAGAATGGGAAATGAATG-3'

[0028] GA19 R: 5'-TCCTTTGGAGTTGTTGGTG-3';

[0029] The specific method is as follows: according to the BAC-terminal sequence of the Narcissus genome, use the SSR Hunter analysis software (microsatellite search tool) to search for...

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Abstract

The invention relates to a narcissus BES-SSR marker primer GA19 and a method for identifying narcissus species. The method comprises the following steps: design and synthesis of the narcissus BES-SSR marker primer GA19, extraction of total DNA (deoxyribonucleic acid) from a narcissus genome, establishment of a PCR (polymerase chain reaction) total reaction system, establishment of PCR amplification programs, establishment of polyacrylamide gel electrophoresis conditions, and detection and identification of PCR amplification products. A narcissus BES-SSR marker developed by a BAC-terminal sequence of the narcissus genome is adopted, has the characteristics of high accuracy, easiness in judgment, good repeatability, effect of not being affected by environmental factors, clear spectral band and the like, is an effective and reliable molecular marker, and further has the advantages of small sampling quantity, convenience in operation, reliable results and the like when used for identifying the narcissus species. The narcissus BES-SSR marker primer GA19 can also be used for drawing of a narcissus species fingerprint spectrum, analysis of genetic diversity, research of a population genetic structure, analysis of a genetic linkage map and other technical fields.

Description

technical field [0001] The invention relates to a BES-SSR marker primer and a method for identifying a crop variety thereof, in particular to a narcissus BES-SSR marker primer GA19 and a method for identifying a narcissus variety, belonging to the field of molecular biology. Background technique [0002] Narcissus is a genus of Amaryllidaceae Narcissus ( Narcissus L .) Perennial flower bulbs, most of which are native to the Mediterranean coast and central Europe. There are about 40 native species and many varieties. According to the statistics of the Royal Horticultural Society, there are now more than 10,000 horticultural varieties. It is very rich, and some daffodils have a pleasant fragrance, and have become famous ornamental and medicinal flowers widely cultivated in countries all over the world. However, narcissus has the characteristic of dormancy in summer and blooms once a year. It takes a long time to identify the flower type and color, which is time-consuming and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 潘东明祁世明潘腾飞郭志雄林琳
Owner FUJIAN AGRI & FORESTRY UNIV
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