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Method for constructing tobacco core germplasm based on genomics and application thereof

A core germplasm and genomics technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem that there is no genomics method for constructing tobacco core germplasm, so as to fully tap the potential of germplasm resources, High repeatability and operability, added effect of sustainable agricultural development

Pending Publication Date: 2021-02-12
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the method of constructing tobacco core collection based on genomics

Method used

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  • Method for constructing tobacco core germplasm based on genomics and application thereof
  • Method for constructing tobacco core germplasm based on genomics and application thereof
  • Method for constructing tobacco core germplasm based on genomics and application thereof

Examples

Experimental program
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Embodiment 1

[0059] (1) Extract the genomic DNA of the original population tobacco samples

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Abstract

The invention discloses a method for constructing tobacco core germplasm based on genomics and application of the method, and belongs to the technical field of molecular biology, genomics and germplasm resource science. The method comprises the steps: performing simplified genome sequencing on tobacco germplasm resource populations, adopting sequencing data quality control, SNP detection screeningand population stratification analysis, further dividing the whole germplasm resource large population into 200 subpopulations through IBS and K-means analysis, combining random selection in the subpopulations with background screening, and performing constructing to obtain the core germplasm capable of representing the whole tobacco germplasm resource large group. According to the method, the core germplasm construction efficiency can be improved, the genetic structure of an original population is well reserved, and the method has important significance in improving the germplasm resource utilization efficiency.

Description

technical field [0001] The invention relates to the technical fields of molecular biology, genomics and germplasm resources, in particular to a method for constructing tobacco core germplasm based on genomics and its application. Background technique [0002] Tobacco is an important economic crop. Through the domestication of various ecological and environmental conditions, artificial selection and cultivation, and the continuous introduction of tobacco germplasm resources from abroad, my country has become the country with the largest number of preserved tobacco germplasm resources and rich genetic diversity in the world. . Although my country has achieved certain results in the collection and utilization of variety resources, it is still relatively weak in the identification of tobacco germplasm resources and genetic diversity research, and no systematic and comprehensive research on resource genetic diversity has been carried out. The existing preserved tobacco germplasm r...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/6895
CPCC12Q1/6869C12Q1/6895C12Q2600/156C12Q2535/122C12Q2537/165
Inventor 刘国祥李洋洋冯全福张兴伟杨佳蒴李尊强佟英周志成胡日生杨爱国戴培刚向世鹏李媛
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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