Narcissus bes-ssr marker primer ag9 and method for identifying narcissus varieties

Abstract

The present invention relates to Narcissus BES-SSR marker primer AG9 and a method for identifying Narcissus varieties, including the design and synthesis of Narcissus BES-SSR marker primer AG9, extraction of total DNA from Narcissus genome, establishment of PCR overall reaction system, PCR amplification program, polyacrylamide Gel electrophoresis conditions and detection and identification of PCR amplification products. The Narcissus BES-SSR marker developed by the BAC-terminal sequence of the Narcissus genome in the present invention has the characteristics of high accuracy, easy judgment, good repeatability, no influence of environmental factors, and clear bands, and is an effective and reliable molecule. The marker is used for the identification of Narcissus germplasm, and has the advantages of small sampling volume, convenient operation, and reliable results. Narcissus BES-SSR marker primer AG9 can also be used in technical fields such as drawing fingerprints of narcissus varieties, analyzing genetic diversity, studying the genetic structure of gregarious populations, and analyzing genetic linkage maps.

Description

technical field [0001] The invention relates to a BES-SSR marker primer and a method for identifying a crop variety thereof, in particular to a narcissus BES-SSR marker primer AG9 and a method for identifying a narcissus variety, belonging to the field of molecular biology. Background technique [0002] Narcissus is a genus of Amaryllidaceae Narcissus ( Narcissus L .) Perennial flower bulbs, most of which are native to the Mediterranean coast and central Europe. There are about 40 native species and many varieties. According to the statistics of the Royal Horticultural Society, there are now more than 10,000 horticultural varieties. It is very rich, and some daffodils have a pleasant fragrance, and have become famous ornamental and medicinal flowers widely cultivated in countries all over the world. However, narcissus has the characteristic of dormancy in summer and blooms once a year. It takes a long time to identify the flower type and color, which is time-consuming and l...

AI Technical Summary

Problems solved by technology

[0006] At present, there are mainly two types of SSR markers developed: G-SSR (genome SSR) and EST-SSR (expressed sequence tag SSR), and the existing Narcissus EST database There are still very few narcissus EST sequences registered in China, and the development of SSR markers is far from enough, and the polymorphism of SSR markers obtained by using EST sequences is lower than that of genomic SSR markers. It is imminent that SSR marker primers provide effective marker technology for the identification of Narcissus germplasm and genetic diversity analysis.

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