Method for classifying ctenopharyngodon idella based on expressed sequence tag-simple sequence repeats (EST-SSR) marker

A classification method and grass carp technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of insufficient research on the genetic structure of grass carp populations.
CN102242222BInactive Publication Date: 2013-05-15PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2013-05-15
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a grass carp classification method based on EST-SSR markers. The method includes extracting the DNA of the grass carp, using EST-SSR markers to amplify, and analyzing the genetic structure of the grass carp according to the amplification result. Compared with the traditional method, the method of the invention has the advantages of strong purpose and direct effect. And the operation is simple, the detection is fast, the detection cost is low, and it is convenient to be popularized and used widely.
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Description

technical field

[0001] The invention relates to a fish classification method, in particular to a grass carp classification method based on EST-SSR markers. Background technique

[0002] grass carp (Ctenopharyngodon idella) It has the advantages of fast growth and low breeding cost. It is the main breeding object in ponds, lakes and reservoirs, and is one of the most important breeding species in my country's freshwater fishery. Grass carp is distributed in all major river systems in Asia, from Heilongjiang to Xijiang in China (except for Xinjiang and the Qinghai-Tibet Plateau where there is no natural distribution), forming a complex genetic background and rich germplasm resources of grass carp. In recent years, the changes and pollution of the natural water environment have caused a significant decline in the number of natural grass carp populations, and inbreeding in production has led to germplasm degradation, which has affected the normal development of the grass carp a...

Claims

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