A method for analyzing genetic diversity of corn borer populations using issr system

A technology of genetic diversity and Asian corn borer, applied in the field of molecular biology, can solve the problem of less research on the genetic diversity of corn borer populations, and achieve high polymorphism, clear amplification fragments, and stable PCR amplification reaction Effect
CN109576377BActive Publication Date: 2022-03-15黑龙江省植检植保站
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
黑龙江省植检植保站
Publication Date
2022-03-15

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Abstract

A method for analyzing the genetic diversity of corn borer population by using ISSR system, which relates to the method of genetic diversity of corn borer population; Corn borer, especially the genetic structure and kinship of different geographical populations of the Asian corn borer, so as to clarify the problem of the migration and change of the corn borer; the invention provides a method for analyzing the genetic diversity of the corn borer population using the ISSR system. This method is a simple and easy-to-operate molecular marker technology. The 14 ISSR primer sequences selected by this method have stable PCR amplification reactions in corn borer, clear amplified fragments, and high polymorphism, which can be used as corn borer resources. Provide technical support for scientific research such as identification and genetic diversity analysis. The invention is applied to the field of corn borer.
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Description

technical field

[0001] The invention belongs to the field of molecular biology, and in particular relates to a method for analyzing the genetic diversity of corn borer populations by using an ISSR system. Background technique

[0002] The vast majority of species in nature will have non-random gene flow due to their differences in behavior and asymmetric environmental factors, resulting in different degrees of genetic differences among species populations in different regions. Due to the phenomenon of differentiation and formation of new species among different populations, the diversity of population genetic structure and population genetics also arise.

[0003] Molecular markers are one of the important technologies for studying population genetics at present. With the rapid development of molecular biology research techniques, various molecular marker technologies have emerged. ISSR molecular markers are derived from SSR molecular markers. With the advantages of SSR mole...

Claims

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