A method for channel catfish microsatellite family identification

Abstract

The invention discloses a microsatellite family identification method for ictalurus punctatus. The microsatellite family identification method comprises the following steps: (1) breeding an ictalurus punctatus family by adopting an artificial propagation method, and carrying out polyculture on 100 family descendants in one pond; (2) analyzing gene types of an ictalurus punctatus breeding group on 18 microsatellite marker sites by adopting a fluorescence marking primer and a multi-PCR (Polymerase Chain Reaction) amplification method; screening 10 effective microsatellite markers which have high polymorphism and are suitable for family identification; (3) optimizing a multi-PCR amplification system of the 10 microsatellite markers and analyzing gene types of the polycultured descendants on 10 microsatellite marker sites; (4) identifying a parental origin of each descendant according to the gene types of parents and the descendants. According to the microsatellite family identification method disclosed by the invention, a paternity testing method is established for the ictalurus punctatus for the first time and the identification accuracy reaches 96.6 percent; polyculture individual parents of the ictalurus punctatus can be rapidly and accurately identified. The microsatellite family identification method disclosed by the invention can be used for rapidly and accurately carrying out family identification on the polyculture individual parents of the ictalurus punctatus, and the breeding effect is improved.

Description

technical field

[0001] The invention belongs to the technical field of molecular markers for fish genetic breeding, and specifically relates to a method for identifying the parents of offspring of channel catfish polyculture families by using fluorescent marker microsatellites. Background technique

[0002] Channel catfish is native to the United States and is an important aquatic economic species. It was introduced to my country in 1984. Because of its tender meat, rich nutrition, high meat yield, and no intermuscular spines, it is very popular among consumers. With the expansion of the breeding scale of channel catfish in my country and the increasing restrictions on the germplasm supply of Chinese channel catfish in the United States, there have been obvious signs of germplasm decline in channel catfish cultured in my country, such as slow growth, body color differentiation, irregular specifications, and frequent disease occurrence. This phenomenon has seriously affected ...

Examples

Embodiment 1

[0050] (1) Establishment of channel catfish core breeding family

[0051] In 2008, a total of 405 channel catfish were introduced from the United States from 1997 to 2004 from the Texas (1997) population, the Arkansas (1999) population, the Mississippi (2001) population, the Arkansas (2003) population and the Arkansas (2004) population to establish a breeding basis group, using the basic group for G 0 Generational family lineage construction. G was selected in June 2011 0 Generation selection breeding population as parents to construct 50 G 1generation family. G was selected in June 2015 1 Generation selection breeding population as parents to construct 100 G 2 generation family. The caudal fin rays used for breeding parents were clipped and stored in 95% alcohol at -20°C. After the channel catfish female parent spawned, the eggs of each family were hatched independently. After the fry hatched for 15 days, 200 fry were randomly selected from each family and mixed in a p...

technical field

[0001] The invention belongs to the technical field of molecular markers for fish genetic breeding, and specifically relates to a method for identifying the parents of offspring of channel catfish polyculture families by using fluorescent marker microsatellites. Background technique

[0002] Channel catfish is native to the United States and is an important aquatic economic species. It was introduced to my country in 1984. Because of its tender meat, rich nutrition, high meat yield, and no intermuscular spines, it is very popular among consumers. With the expansion of the breeding scale of channel catfish in my country and the increasing restrictions on the germplasm supply of Chinese channel catfish in the United States, there have been obvious signs of germplasm decline in channel catfish cultured in my country, such as slow growth, body color differentiation, irregular specifications, and frequent disease occurrence. This phenomenon has seriously affected ...

Examples

Embodiment 1

[0050] (1) Establishment of channel catfish core breeding family

[0051] In 2008, a total of 405 channel catfish were introduced from the United States from 1997 to 2004 from the Texas (1997) population, the Arkansas (1999) population, the Mississippi (2001) population, the Arkansas (2003) population and the Arkansas (2004) population to establish a breeding basis group, using the basic group for G 0 Generational family lineage construction. G was selected in June 2011 0 Generation selection breeding population as parents to construct 50 G 1generation family. G was selected in June 2015 1 Generation selection breeding population as parents to construct 100 G 2 generation family. The caudal fin rays used for breeding parents were clipped and stored in 95% alcohol at -20°C. After the channel catfish female parent spawned, the eggs of each family were hatched independently. After the fry hatched for 15 days, 200 fry were randomly selected from each family and mixed in a p...

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