Molecular marker method for breeding meat performance of Aletail sheep and application thereof

A molecular labeling and performance technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems that have not been seen, not reported, and applied research has not been reported, and achieve far-reaching technical contributions and practical effects Effect

Active Publication Date: 2014-01-22
XINJIANG AGRI UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are no studies and reports on the use of candidate gene strategies to improve the meat production traits of Altay sheep at home and abroad, which is completely blank
In addition, as for the MyoD1 gene as a candidate gene affecting the production performance of livestock, the relevant research reports are mostly found in goats, cattle and pigs, but not in sheep, and there is no report on the application of MyoD1 in Altay sheep

Method used

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  • Molecular marker method for breeding meat performance of Aletail sheep and application thereof
  • Molecular marker method for breeding meat performance of Aletail sheep and application thereof
  • Molecular marker method for breeding meat performance of Aletail sheep and application thereof

Examples

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Embodiment 1

[0040] Example 1: Method for Screening DNA Molecular Markers Significantly Correlated with Altay Sheep Meat Production

[0041] A molecular marker method for breeding meat production performance of Altay sheep, using PCR-SSCP technology to screen DNA molecular markers significantly related to meat production of Altay sheep, the main invention points are as follows:

[0042] 1. Genomic DNA extraction

[0043] Collect 5ml of blood from the jugular vein of the Altay sheep to be tested, anticoagulate with sodium citrate, and store at -20°C;

[0044] According to the "Molecular Cloning Experiment Guide", the genomic DNA was extracted with phenol-chloroform extraction and stored at -20°C.

[0045] Prepare 0.8% agarose gel: Dissolve 0.8g of agarose in 100m10.5×TBE, heat it in a microwave oven until it is fully dissolved, pour it into a gel tank with a comb inserted after cooling down to room temperature, cool and solidify before use.

[0046] The prepared 0.8% agarose gel was used ...

Embodiment 2

[0076] Example 2: Using the molecular marker MyoD1 gene selection to increase the meat production of Altay sheep

[0077] Collect 5ml of blood sample from the individual Altay sheep to be tested, and extract genomic DNA; obtain the sequence of primer 1, and carry out PCR amplification, wherein the annealing temperature is 52°C; the amplified product is denatured and then detected by SSCP; figure 2 Determine genotype.

[0078] Individuals of Altay sheep whose genotype is TT or TC can be eliminated; individuals of Altay sheep whose genotype is CC can be retained, so as to improve the meat yield of Xinjiang Altay sheep.

[0079] As mentioned above, in the present invention, in constructing a molecular marker method for breeding meat production performance of Altay sheep, the technical steps adopted involve the use of the well-known biological analysis software SPSS17.0 for significance testing, and the applied molecular marker Technology PCR-SSCP (Single Strand Conformation Pol...

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Abstract

The invention discloses a molecular marker method for breeding meat performance of Altay sheep and application thereof. The molecular marker method comprises the following steps: using a MyoD1 (Myogenic Determination) gene as a candidate gene which influences meat yield of the Altay sheep; detecting a genetic structure of the MyoD1 gene in the Altay sheep variety by utilizing a PCR-SSCP (Polymerase Chain Reaction-Single-strand Conformation Polymorphism) technology; analyzing and judging a correlation between the genetic structure of the MyoD1 gene and the meat yield of the sheet; by a DNA (Deoxyribose Nucleic Acid) sequence of the MyoD1 gene of the sheet, self-designing two pairs of specific primers; carrying out polymorphism detection on the MyoD1 gene of the Altay sheep in Sinkiang province by utilizing the PCR-SSCP technology; and carrying out correlation analysis with meat performance so as to find a molecular marker which is obviously related to the meat yield of the Altay sheep. The molecular marker method is used for improving meat performance of the Altay sheep to greatly improve the speed of improving and recovering excellent meat performance of the Altay sheep and has important significance and practical application value for improving economic income of farmers and herdsmen and quickening development of the mutton sheep industry in Sinkiang province.

Description

field of invention [0001] The invention relates to the technical field of animal molecular genetics and breeding, in particular to the technical field of using molecular biotechnology to find effective molecular markers that affect the meat production traits of Xinjiang Altay sheep, and then applying it to improve the meat production performance of Altay sheep. Background technique [0002] Xinjiang is one of the main pastoral areas in my country. It has abundant sheep breed resources. Among them, the Altay sheep is famous for its large physique and high meat fat production performance. Excellent mutton sheep breed in Xinjiang. However, ethnic minorities account for the vast majority in the Altay region of Xinjiang. The winter lasts for nearly half a year, and the coldest temperature can reach -35-40°C. Due to the harsh natural conditions, many fine varieties cannot survive here. The Altay sheep was formed under such a special condition through long-term natural and artifici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘武军王琼邵勇钢刘玲玲马海玉于茜
Owner XINJIANG AGRI UNIV
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