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Polymorphic primer of cinnamomum camphora nuclear genome SSR molecular marker and application thereof

A technology of polymorphism primers and nuclear genome, applied in the direction of recombinant DNA technology, microbial measurement/testing, biochemical equipment and methods, etc., can solve the problem of low polymorphism, poor repeatability, and difficulty in fully revealing the genetic diversity of Cinnamomum camphora Real level and other issues, to achieve the effect of good repeatability and wide coverage

Active Publication Date: 2020-01-24
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI +1
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Problems solved by technology

In addition, in terms of research methods, the co-dominant DNA markers such as RAPD and ISSR used in the early stage have poor repeatability and low polymorphism, and it is difficult to fully reveal the true level of the genetic diversity of Cinnamomum camphora.

Method used

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  • Polymorphic primer of cinnamomum camphora nuclear genome SSR molecular marker and application thereof
  • Polymorphic primer of cinnamomum camphora nuclear genome SSR molecular marker and application thereof
  • Polymorphic primer of cinnamomum camphora nuclear genome SSR molecular marker and application thereof

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Embodiment 1

[0039] 1) Test materials

[0040] The test materials come from 18 groups in the natural distribution area of ​​Cinnamomum camphora, namely Luzhou in Sichuan, Jing'an in Jiangxi, Ningdu in Jiangxi, Yibin in Sichuan, Daozhen in Guizhou, Guilin in Guangxi, Enjiang in Jiangxi, Longgang in Jiangxi, Anqing in Anhui, Le'an in Jiangxi, Ruihong in Jiangxi, Pingxiang in Jiangxi, Guangzhou in Guangdong, Nanchang in Jiangxi, Guiyang in Guizhou, Libo in Guizhou, Wuyishan in Fujian and Nanjing in Jiangsu. Ancient camphor trees with a breast diameter greater than 60 cm were selected, and 5-10 fresh leaves were collected from each individual and transported on dry ice, quickly frozen in liquid nitrogen and stored in a -80°C refrigerator. The specific location, latitude and longitude, and number of samples of the collection group are shown in Table 1.

[0041] Table 1 Sampling information of 18 ancient camphor populations

[0042] source sample size group LongitudeE° Latitu...

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Abstract

The invention discloses a polymorphic primer of a cinnamomum camphora nuclear genome SSR molecular marker and application thereof, and belongs to the technical field of forestry molecular biology. Thenucleotide sequence of the primer is shown as SEQ ID NO.1 to SEQ ID NO.14. The polymorphic primer is applied to the application of variety identification of cinnamomum camphora, and genetic structureand resource genetic diversity analysis of ancient cinnamomum camphora groups. A method includes the steps of genome DNA extraction, PCR amplification, polyacrylamide gel electrophoresis and data analysis. 186 individuals of 18 ancient cinnamomum camphora groups are subjected to genetic structure and resource genetic diversity study; the possibly forming reasons are analyzed according to existinggenetic structure and resource genetic diversity patterns of the cinnamomum camphora; and theoretical basis is provided for the wild cinnamomum camphora resource protection and utilization. The repeatability of the SSR molecular marker is high, and the real level of the genetic diversity of the cinnamomum camphora can be comprehensively disclosed.

Description

technical field [0001] The invention belongs to the technical field of forestry molecular biology, and in particular relates to polymorphic primers for SSR molecular markers of Cinnamomum camphora nuclei genome and applications thereof. Background technique [0002] Cinnamomum camphora (L.) Presl is a representative species of subtropical evergreen broad-leaved forest, widely distributed in the south of the Yangtze River in my country. Cinnamomum camphora has a long history of cultivation and utilization in my country, and the resources of ancient camphor are rich, containing valuable genetic resources, but there are few reports on the level of genetic variation and its phylogenetic relationship. [0003] Based on the research results of DNA molecular markers, the conclusions are different. Yao Xiaohua used 9 pairs of RAPD primers to analyze the genetic diversity of 82 families of Cinnamomum camphora from 10 provenances. A total of 146 polymorphic bands were amplified. The ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 钟永达杨茂霞胥猛余发新郦芝汀
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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