Primers and method for constructing

Abstract

The invention discloses primers and a method for constructing cynodon dactylon core germplasm by using a SCoT molecular marker. The sequences of the primers are shown as SEQ ID NO: 1-19, and the primers are applied to construction of the cynodon dactylon core germplasm. The 19 primer strips disclosed by the invention are clear, high in polymorphism and good in repeatability, and a cynodon dactylonSCoT amplification result shows that the 19 primer strips have rich genetic diversity and are suitable for constructing core germplasm. The cynodon dactylon SCoTCore disclosed by the invention generally maintains the genetic structure of an original population, the redundant materials are effectively removed, and the cynodon dactylon SCoTCore is a qualified core germplasm and is representative ofthe original germplasm.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer and a method for constructing bermudagrass core germplasm using SCoT molecular markers. Background technique [0002] Bermudagrass (Cynodon Richard) belongs to the C4 perennial herb of the Poaceae Teffaceae Sansevieria family. It is one of the three warm-season turfgrasses in the world and is also an excellent pasture (Harlan, 1969). There are 9 kinds and 10 varieties (Taliaferro, 1995). Most bermudagrass originated in Africa, mainly distributed in warm and humid tropical or subtropical regions, and some bermudagrass were distributed in temperate regions (Rochecouste, 1962; Wofford and Baltensperger, 1985; Harlan, 1970). Among them, common bermudagrass (C.dactylon var.dactylon) is a widely distributed species in the world, with a horizontal distribution range of 53°N-45°S; vertical distribution, it can grow both below sea level and on the Himalayas at an altitude of 4000m ...

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Problems solved by technology

However, the application of SCoT molecular m...

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