97 results about "In vivo degradation" patented technology

The invention relates to an endovascular implant, which is at least largely biodegradable and whose in vivo degradation can be controlled. To achieve this, the implant comprises a tubular base body, open on its end faces and consisting of at least one biodegradable material, said base body having an in vivo, location-dependent first degradation characteristic D₁(x), in addition to a coating that covers the base body completely or in sections and consists of a biodegradable material, said coating having an in vivo, location-dependent second degradation characteristic D₂(x). According to the invention, a location-dependent cumulative degradation characteristic D(x) in one location (x) is made up of the sum of the respective degradation characteristics D₁(x) and D₂(x) in said location (x) and the location-dependent cumulative degradation characteristic D(x) is predetermined by a variation of the second degradation characteristic D₂(x) in such a way that the degradation in the given location (x) of the implant takes place over a predeterminable time period at a predeterminable degradation rate.

The invention relates to a medical wound surface hemostatic and wound healing agent, which is characterized in that the invention consists of two parts of water solution, one part is the oxidative polysaccharide water solution containing dialdehyde, and the other part is the water-soluable chitosan water solution. The medical wound surface hemostatic and wound healing agent can be applied in the preparation of drugs or medical devices for wound surface sealing and wound surface hemostasis, and the invention can also be applied in the preparation of drugs or medical devices for the promotion of wound surface healing and the prevention of postoperative tissue adhesion. The medical wound surface hemostatic and wound healing agent of the invention has significant effects on wound surface sealing, hemostasis, promotion of wound surface healing, prevention of postoperative tissue adhesion and other aspects, at the same time, the biocompatibility is good, which can not cause the tissue immune response, and the polysaccharides can not generate side effects on organisms after the in vivo degradation, so the safety is good.

The invention provides a double-layered bone repairing membrane material and a preparation method thereof and in particular provides a bone repairing membrane material with antibacterial and inflammation-diminishing and bone promotion functions and belongs to the field of biological materials. The material takes biodegradable aliphatic polyester with biocompatibility and natural polymers as main raw materials and is prepared by adopting an electrostatic spinning method. The material has a double-layered membrane structure and comprises an outer layer added with an antibacterial drug and an inner layer added with a bone promotion substance. The material provided by the invention has excellent biocompatibility and a controllable and long-period medicine releasing performance; meanwhile, an outer-layer membrane of the double-layered membrane structure can be used for inhibiting bacterial infection and inflammation, which are easily caused after bone defects occur, and preventing bacteria from entering defected parts; an inner-layer membrane can be used for promoting the repairing of the bone defects. The material can be used for realizing controllable in-vivo degradation according to requirements and the material does not need to be taken out by a second surgery.

The present invention relates to technical field of biological material, and is especially suitable for surface modification field of biological medical material. Particularly the invention provides a medicine-taking degradable magnesium alloy cardiovascular bracket and a preparing method thereof. The aim of the invention is to reduce the initial degrading speed of magnesium alloy in biosome and carry therapeutic medicine on the surface of magnesium bracket thereby guaranteeing the mechanical performance of magnesium alloy bracket in vivo and better restraining the hyperplasia of newborn tunica interna. A fluoro-containing transition film is prepared on the surface of magnesium alloy bracket, and a medicine-carrying layer is prepared on the outer layer of magnesium alloy bracket. A protecting layer is the fluoro-containing transition film. The fluoro-containing transition film has self-concrescence capability in the degradation process of magnesium alloy and can effectively reduce the in-vivo degradation velocity of magnesium alloy bracket. The surface of degradable magnesium alloy cardiovascular bracket is smooth and is not easily generated with surface cracking and breaking-off. The medicine-taking layer is composed of medicine carrier polymer or protein and therapeutic medicine. The medicine-taking layer can release therapeutic medicine, reduce the generation rate of stenosis after the implantation of bracket and has a control function to the degradation velocity of magnesium alloy bracket.

The invention provides a method for preparing a cross-linked hyaluronan sponge, and the sponge obtained by the method. The method comprises the following steps of: (1) swelling a hyaluronan raw material in water to obtain a hyaluronan gel fluid; (2) adjusting the hyaluronan gel fluid to be alkaline, adding a cross-linking agent, and performing cross-linking reaction to obtain a cross-linked hyaluronan gel; and (3) cleaning the cross-linked hyaluronan gel, and freeze-drying at a low temperature to obtain the cross-linked hyaluronan sponge. The cross-linked hyaluronan sponge prepared by the method provided by the invention is low in the content of the cross-linking agent, good in biocompatibility, consistent in in-vivo degradation cycle and tissue healing cycle, and good in blood-stopping and anti-adhesion functions.

The invention provides a magnesium-zinc-copper alloy. The magnesium-zinc-copper alloy comprises 1-8wt% of zinc, 3wt% or less of copper, and the balance of magnesium. The magnesium-zinc-copper alloy has good biocompatibility, satisfactory degradability, high biological safety and extremely good biomechanical performances, can continuously slowly release metal ions having an antibiotic effect to surrounding tissues through in vivo degradation in order to effectively prevent and treat implant surrounding infection, has certain hematopoietic activity, and has good application values in implanting into bones and other medical fields.

The invention discloses a method for promoting in-vitro and in-vivo degradation and cell extension adherence of alginate-based 3D printed bioink. The method comprises the following steps: step 1, adopting alginate as an extracellular matrix main body skeleton and constructing celliferous 3D bio-printed bioink; step 2, mixing alginate lyase with alginate-based bioink; step 3, adding cell suspensioninto the obtained bioink, choosing print parameters and printing out a 3D printing block through a 3D bio-printer; step 4, regulating and controlling in-vitro and after-transplantation-in-vivo degradation time and cell extension adherence degree of the 3D printing block through adjusting the concentration of the alginate lyase according to actual demands. The method disclosed by the invention canpromote the degradation and cell extension adherence of the bioink with the alginate as the skeleton under in-vivo and in-vitro environments, and enables the alginate 3D printed bioink to be adaptedto more in-vivo and in-vitro experiments and be better applied to soft tissue repairing and transplantation.

The invention relates to a biodegradable nanometer fiber diaphragm applied to neurosurgery indirect vascular bypass. The biodegradable nanometer fiber diaphragm can be produced through the following steps: dissolving a biodegradable polymer material in a polar and volatile organic solvent, carrying out electrostatic spinning to produce a diaphragm composed of ultrafine fibers with the nano-scale diameters; and loading growth factors for promoting brain tissue blood vessel regeneration and/or promoting nerve regeneration to the diaphragm through a layer and layer self-assembling technology, and drying the factor growth supported diaphragm. The biodegradable nanometer fiber diaphragm has the advantages of no toxicity, no generation of harmful substances after in vivo degradation, and good biocompatibility. The biodegradable nanometer fiber diaphragm can avoid growth factor inactivation caused by organic solvents, strong acids, strong alkalis and high temperature, and an assembled polyelectrode can guarantee the good activity of the growth factor supported on the diaphragm. The biodegradable nanometer fiber diaphragm can realize long-term controlled release of VEGF, NGF and other growth factors in the brain blood vessel obstruction position in order to induce blood vessel regeneration and nerve regeneration of the brain tissue blood vessel obstruction position.

The invention discloses an in vivo absorbable metal intervertebral fusion cage, the fusion cage is made of high-purity magnesium or magnesium alloy, the purity of the high-purity magnesium is not less than 99.9%, the content of the magnesium in the magnesium alloy by weight is 10%-100%, the total content of an alloy element is 0-90%, and the content of other impurity ingredients is not more than 5%; and the alloy element comprises one or more of Zn, Ca, Mn, Fe, Al and rare earth. The in vivo absorbable metal intervertebral fusion cage can be gradually degraded and absorbed in vivo, and the elastic modulus is similar to that of bones, thereby making up for disadvantages that the fusion cage is sunk due to stress shielding effect of titanium alloy material and the like, and the loss of intervertebral support height is caused. Compared with a permanently implanted PEEK (polyetheretherketone) fusion cage, the in vivo absorbable metal intervertebral fusion cage can promote bone repair and enable bone absorption and formation of new bones to be in good matching during the in vivo degradation process; simultaneously, the support height is maintained, the intervertebral bone union can be realized without leaving foreign bodies, the second taking-out can be avoided, and the suffering of a patient can be greatly reduced.

The invention relates to a guided tissue regeneration membrane and a preparation method thereof. The method comprises: a pretreatment step, i.e. collecting a mammalian peritoneum, conducting flowing water washing, hair removal and preliminary degreasing for standby use; an antigen removal treatment step, i.e. carrying out further degreasing on the standby peritoneum by means of an enzyme treatment process, an alkali treatment process, an acid treatment process and a hypertonic treatment process, and removing immunogenicity; a collagen fiber contraction treatment step, i.e. carrying out dehydration, non-hydrophilic substance removal and washing treatment on the peritoneum subjected to the antigen removal treatment so as to contract the collagen fibers of the peritoneum; and a drying step, spreading the contracted peritoneum on a culture dish surface to make the compact layer of the peritoneum contact the culture dish, rapidly transferring the peritoneum into a refrigerator at a temperature ranging from -60 to -80DEG C to undergo pre-freezing for 1-8h, then freeze-drying the peritoneums in a freeze dryer for 24-36h, thus obtaining a collagen membrane able to guide tissue regeneration. The guided membrane provided by the invention has a compact layer and a loose layer, also has good biocompatibility, hydrophilicity and bone induction ability, and has a long in-vivo degradation time.

The invention belongs to the technical field of biomedical materials and tissue engineering technologies, and particularly relates to hyaluronic acid-methyl cellulose composite hydrogel as well as preparation and an application thereof. The hyaluronic acid-methyl cellulose composite hydrogel comprises methyl cellulose and hyaluronic acid in the mass ratio of (5-11):1 and adopts a crosslinking network structure, and the pore diameter ranges from 30 mu m to 100 mu m. The methyl cellulose with temperature-sensitive characteristic, good biocompatibility and small in-vivo degradation speed and hyaluronic acid molecules have a physical crosslinking reaction through polyethylene glycol, and the hyaluronic acid-methyl cellulose composite hydrogel is obtained. The composite hydrogel can be formed at the body temperature by controlling the concentration of the methyl cellulose and salt additives, the mechanical performance is improved, bioactive molecules can be controlled to be released, the in-vivo retention time is prolonged, regeneration and repair of soft tissue similar to nervous tissue are facilitated, and the hyaluronic acid-methyl cellulose composite hydrogel has wide application prospect.

The present invention provides a biomedical degradable metal capable of treating rheumatoid arthritis. The biomedical degradable metal is a magnesium alloy and contains magnesium and a second component, wherein the second component is one or a plurality of materials selected from zinc, manganese, selenium, strontium and calcium, the content of the second component is less than or equal to 10 wt%, and the balance is magnesium. According to the present invention, the magnesium alloy has characteristics of good biocompatibility, satisfactory corrosion resistance and no significant cytotoxicity, can be degraded and absorbed in biological body fluids or bloods, has application values in medical fields of endovascular interventional therapy, endosseous implant and the like, and especially releases beneficial elements through in vivo degradation so as to inhibit activation of macrophages and other inflammatory cells, promote osteoblast bone formation, and inhibit osteoclast bone resorption, such that the biomedical degradable metal has the potential effects of rheumatoid arthritis treating and the like.

The invention provides an injectable hydrogel, and a preparation method thereof. The injectable hydrogel is obtained via self-crosslinking reaction of arginine-modified chitosan with a structure represented by formula (I) with a cross-linking agent; the polymerization degree n of the arginine-modified chitosan is equal to or higher than 10, and is equal to or lower than 3200; the arginine substitution degree ranges from 0.1 to 80%; the cross-linking agent is oxidized dextran. Compared with the prior art, the injectable hydrogel possesses following advantages: the biocompatibility is high; in vivo in-suit formation, and in vivo degradation can be realized; it is beneficial for direct acting of drugs at diseased regions, and long lasting of drug effect; oxidized dextran is safe and notoxic as the cross-linking agent; environment pollution is reduced; the degradation products are amino acids and polysaccharides, and can be discharged out of human body via kidneys, so that the injectable hydrogel is harmless for human body.

The invention provides a degradable gasket with anti-bacterial anti-adhesion performance. The degradable gasket is prepared via weaving or knitting of a plurality of bundles of magnesium-based metal wire, or via punching of magnesium-based metal sheets. The magnesium-based metal wire can be made of pure magnesium or a magnesium-based alloy of different elements based on disease characteristics and treatment requirements. The degradable gasket possesses excellent biocompatibility; in vivo degradation and absorption can be realized; degradation speed can be controlled; the degradable gasket is capable of preventing postoperative adhesion and infection, and promoting wound healing, can be used for preventing epidural adhesion after orthopedic surgery laminectomy, adhesion after tendon rupture repairing operation, adhesion after knee surgery, dura adhesion after transcranial surgery, eye socket soft tissue adhesion, intestinal adhesion after abdominal surgery, and intrauterine adhesion, is capable of reducing infection risk of the above surgeries, and possesses significant clinical application value.

The invention relates to a tissue sealing agent powder and a preparation process thereof, and a tissue sealing agent. The sealing agent powder comprises a first component and a second component, the first component is an electrophilic raw material and contains an electrophilic functional group or a physically loaded electrophilic functional group compound; the second component is a botanical biological material; and a mass ratio of the first component to the second component is 1: 1-10. The preparation method adopts a physical melting method and a fluidized bed method. According to the preparation method, high-water-absorptivity substances of the first component and the second component contain active functional groups without any other catalysts and reagents or under a condition of changing self chemical properties, and the obtained tissue sealing agent has advantages of rapid liquid absorption, a high liquid absorption capacity, a high adhesion capacity, in-vivo degradation and a light inflammatory reaction, and can be directly applied to wounds.

The invention discloses a preparation method of a silk fibroin material of composite nano-grade hydroxyapatite and application of the silk fibroin material in repairing bone fracture parts. The preparation method comprises the following steps: uniformly dispersing nano-grade hydroxyapatite into silk fibroin in a certain ratio, dissolving the nano-grade hydroxyapatite with hexafluoroisopropanol, pouring a mixed solution into a columnar mold, soaking with methanol, performing self-assembling regeneration on a silk fibroin molecular chain so as to obtain a composite material which is excellent inmechanical strength, and finally manufacturing medicinal bone nails from the composite material by using a mechanical processing method. According to the characteristics that the material is good inbiocompatibility, excellent in mechanical property and good in in-vivo degradation controllability, the silk fibroin material can be applied to bone fracture fixation. A silk fibroin/nano-grade hydroxyapatite composite bone fracture internal fixation material with in-vivo degradation controllability can be prepared by using a method which is simple and feasible, high in finished product rate and free of toxicity, osteoporosis symptoms caused in the bone fracture repairing process can be effectively avoided, and because of the characteristic that the material does not need to be taken out through a second time of operation, bone fracture patients can be relieved from pain.

The invention relates to medical hemostatic sponge and a preparation method thereof. The hemostatic sponge is formed by bonding freeze-dried chitosan sponge and carboxymethyl chitosan sponge, and tertiary butanol is added to freeze-dried chitosan sponge and carboxymethyl chitosan sponge in the preparation process. The hemostatic sponge prepared through the process is good in crystal type, short in in-vivo degradation cycle, capable of continuously and effectively adhering to wounds, strong in dissolution resistance, good in hemostatic effect, high in production efficiency, free of toxic and side effects and free of irritation.

The invention discloses a polyester carbonic ester anhydride 3D printing bio-ink, and a 3D printing method. The polyester carbonic ester anhydride 3D printing bio-ink is composed of a gel ink and a support ink; the gel ink is composed of a hydrogel and biological cells; the hydrogel contains a PLGA-PEG-PLGA triblock copolymer with temperature responsiveness; the support ink is a polyester carbonic ester anhydride copolymer (P(LLA-TMC-SA)copolymer) with surface degradation characteristics. The 3D printing bio-ink possesses thermosensitivity and surface degradable performance, and multicomponent gradient printing and coaxial printing are adopted based on requirements of 3D biologically printed scaffold in vivo degradation structure controllable maintenance so as to obtain models which contain biological cells and are supported by scaffolds with surface controllable degradability, nutrient and metabolism channels with porous structures are obtained, and it is beneficial for obtaining of larger and thicker tissue structures via 3D printing.

The invention relates to a positioning marker made of degradable metal and a method for preparing the positioning marker. The positioning marker is made of pure magnesium or magnesium alloy. The totalcontent of impurities in the pure magnesium and the magnesium alloy is lower than 0.01% by mass fraction. At least three magnesium wires are woven to obtain the positioning marker. The positioning marker is provided with a wound end and a positioning end. The positioning end is connected to the wound end and is of a claw-shaped or anchor-shaped structure. The positioning marker and the method have the advantages that the tensile strength of the positioning marker is higher than 200 megapascal, the percentage elongation after fracture is higher than 10%, and the in-vivo degradation rate is lower than 0.5 mm/year; the positioning marker can be developed in ultrasonic and X rays, the locations of the positioning marker can be detected by the aid of ultrasonic or molybdenum target X rays in operative procedures and postoperative treatment procedures, and the positioning marker can be gradually degraded along with postoperative treatment, and can be absorbed by surrounding tissues or discharged from human bodies by means of metabolism without being taken out by means of second operation.

The invention relates to a preparation method of a tubular silk fibroin/keratin composite nanofiber material. The preparation method comprises the steps of adding silk fibroin into a solvent and stirring until dissolving completely to obtain silk fibroin solution with the concentration of 5-20mg/ml; adding keratin into the solvent and stirring until dissolving completely to obtain keratin solution with the concentration of 10-20mg/ml; mixing the silk fibroin solution with the keratin solution in a mass ratio of 95:5-5:95, stirring to obtain a spinning solution, then performing electrostatic spinning and using a cylindrical collecting roller as a receiver. The tubular material has the advantages of adjustable inside dimension, good biocompatibility and in-vivo degradation, and is expected to be used in the field of biological medical materials such as artificial blood vessels and urethra substitutions.

The invention relates to a preparation method of bioactive gradient hard tissue replacement material. Firstly, the pure FHA target material and the FHA plus YSZ compound target materials are prepared by vacuum hot-pressing sintering; the medical metal substrate test sample which is obtained by wire cutting is polished by a metallographical sand paper and then washed, and the substrate after washing is firstly processed by acid etching and then the alkali-heat treatment, so as to obtain the substrate which is processed by activation; the FHA target material, the self-made various groups of compound target materials with different components (FHA plus YSZ) and the medical metal substrate are respectively arranged in a main sputtering chamber and a sample introduction chamber to carry out magnetron sputtering, and post-treatment is carried out in a heat treatment furnace after the completion of magnetron sputtering, then the artificial bone implant which is based on the medical metal surface (FHA plus YSZ) gradient biological coating is obtained. The adhesive strength of the gradient biological coating of the gradient hard tissue replacement material (FHA plus YSZ) which is prepared by the invention and the substrate is high, the temperature increase of the medical metal substrate is low, the components of the coating are even and stable, and the cost is lower; the gradient coating is thinner, the in vivo degradation speed of the FHA gradient coating is lower, the retention time is longer, and the invention is more conductive to the repair and reconstruction of the bone tissues with defects.

The invention discloses a preparation method of an absorbable haemostasis material. The preparation method comprises the following steps: carrying out a cross-linking reaction on a raw material hyaluronic acid or hyaluronate under the action of low-temperature intermittent ultrasonic treatment, uniformly mixing the crosslinked hyaluronic acid or hyaluronate with low-temperature intermittent ultrasonic treatment unreacted hyaluronic acid or hyaluronate, and carrying out ultralow-temperature freeze drying to obtain the absorbable haemostasis material. The low-temperature ultrasonic dissolving technology greatly shortens the hyaluronic acid dissolving time and reduces the pollution probability of the product in the production process; high-molecular weight hyaluronic acid or hyaluronate which can prolong the in-vivo degradation time through improving the solution concentration to meet the wound healing time has large production cost, and is bad for the reduction of the production cost of enterprises; and medium-and-small-molecular weight hyaluronic acid or hyaluronate undergoes the chemical crosslinking reaction under the low-temperature intermittent ultrasonic conditions, and the crosslinked hyaluronic acid or hyaluronate is mixed with the low-temperature intermittent ultrasonic treatment unreacted hyaluronic acid or hyaluronate according to a certain ratio, so the obtained haemostasis material has the advantages of improvement of the anti-adhesion performance, low residual amount of a crosslinking agent, stabilization of the residual amount in a certain range, reduction of the biological risk brought by free crosslinking agents, and reduction of the production cost of the enterprises.

The invention relates to a degradable porous iron-based bone fracture plate with a pore-forming agent and an additional material manufacturing method of the bone fracture plate. The bone fracture plate comprises a main plate body part and side wing parts, and the main plate body part is a porous unit cell structure array composed of multiple micro fine cylinders and is used for fitting the skeleton of a fractured part; the whole porous unit cell structure is of a hexahedral structure, the centers of the adjacent faces of the hexahedral structure are connected through the first micro fine cylinders, the diagonals of all the faces in the hexahedral structure are connected through the second micro fine cylinders, and vertical lines of the hexahedral structure are connected through the third micro fine cylinders; the side wing parts are arranged at the two sides of the main plate body part and used for fixing the main plate body part and the skeleton of the fractured part; micro holes withthe diameter of 1-50 microns are distributed in the main plate body part and the side wing parts. The bone fracture plate has proper in-vivo degradation speed and mechanical properties.

The invention provides a radioisotope labeled meso-porous bioglass porous scaffold. A meso-porous bioglass porous scaffold is labeled with radioisotope, and the radioisotope can be one or more of <31>Si, <32>P and <45>Ca. The radioisotope labeled meso-porous bioglass porous scaffold can effectively label the meso-porous bioglass porous scaffold, can quantitatively and intuitively detect the in vivo degradation situation of the meso-porous bioglass porous scaffold, and can be used to research the in vivo absorption and the organism distribution of the bioglass scaffold.

The invention relates to a method for preparing water-insoluble collagen by using thoracic aortas of pigs as raw materials through process steps such as degreasing, impurity removal, enzyme hydrolysis, salt induced precipitation and dialysis. The process steps of the method are simple, safe and easy to operate, the cost is low and the purity of the obtained product is high. Compared with water-soluble collagen, the in-vivo degradation speed of the water-insoluble collagen obtained by the invention is reduced, the blood coagulation speed is faster and the water-insoluble collagen can be used for stopping wound bleeding or preparing other medical biological materials.