Injectable hydrogel, and preparation method

A technology for injecting water and gel, which is used in pharmaceutical formulations, medical preparations with inactive ingredients, aerosol delivery, etc. Good compatibility, good biodegradability, good biocompatibility

Inactive Publication Date: 2017-06-20
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, biocompatible hydrogel-loaded protein drugs (Chemical Society Reviews, 2012, 41, 4860-4883) can be directly injected into the lesion to achieve sustained release of the drug, prolong the half-life of the drug, and effectively relieve the pain of the patient. But faced with the problem of cumbersome preparation process
With the deepening of scientific research, researchers have prepared block polymer-based hydrogels (Journal of Controlled Release, 2002, 85, 51-59), which are temperature-sensitive, cy

Method used

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  • Injectable hydrogel, and preparation method
  • Injectable hydrogel, and preparation method
  • Injectable hydrogel, and preparation method

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preparation example Construction

[0061] In the present invention, the preparation method of the arginine-modified chitosan preferably comprises the following steps:

[0062] a) mixing chitosan, arginine derivatives and an activator for amidation reaction to obtain a compound shown in formula (II);

[0063]

[0064] The arginine derivative has a structure shown in formula (III):

[0065]

[0066] b) deprotecting the compound represented by formula (II) to obtain arginine-modified chitosan.

[0067] In the present invention, chitosan, arginine derivatives and activators are firstly mixed for amidation reaction to obtain the compound represented by the formula (II). In the present invention, described chitosan has structure shown in formula (IV):

[0068]

[0069] In the present invention, the molecular weight of the chitosan is preferably 2000Da to 500000Da, more preferably 8000Da to 300000Da, more preferably 10000Da to 200000Da; Commercially available products well known to those skilled in the art...

Embodiment 1

[0112] (1) Weigh 2g chitosan and mix it with 200mL hydrochloric acid solution to obtain the first mixed solution; then weigh 0.2g arginine derivative, 0.2g EDC, 0.1g NHS in a round bottom flask, add 20mL DMF to dissolve it , to obtain a second mixed solution; adding the second mixed solution to the first mixed solution, stirring and reacting at 30° C. for 48 hours, and purifying to obtain a solid powder;

[0113] (2) The solid powder obtained in step (1) is dispersed as the reaction medium with 100mL trifluoroacetic acid, 15mL of hydrogen bromide solution is added, reacted for 6h at 30°C, and purified to obtain arginine-modified chitosan, the yield is 80%.

[0114] Carry out nuclear magnetic resonance analysis to the solid powder that step (1) obtains, such as figure 1 shown. The results showed that arginine was successfully attached to chitosan, and the substitution degree of arginine was 3%.

Embodiment 2

[0116] (1) Mix 2g chitosan with 200mL hydrochloric acid solution to obtain the first mixed solution; then weigh 0.4g arginine derivative, 0.4g EDC, 0.2g NHS in a round bottom flask, add 20mL DMF to dissolve it , to obtain a second mixed solution; adding the second mixed solution to the first mixed solution, stirring and reacting at 30° C. for 48 hours, and purifying to obtain a solid powder;

[0117] (2) The solid powder obtained in step (1) is dispersed as the reaction medium with 100mL trifluoroacetic acid, 15mL of hydrogen bromide solution is added, reacted for 6h at 30°C, and purified to obtain arginine-modified chitosan, the yield is 85%.

[0118] The solid powder obtained in step (1) was subjected to nuclear magnetic resonance analysis, and the results showed that arginine was successfully connected to chitosan, and the substitution degree of arginine was 6%.

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Abstract

The invention provides an injectable hydrogel, and a preparation method thereof. The injectable hydrogel is obtained via self-crosslinking reaction of arginine-modified chitosan with a structure represented by formula (I) with a cross-linking agent; the polymerization degree n of the arginine-modified chitosan is equal to or higher than 10, and is equal to or lower than 3200; the arginine substitution degree ranges from 0.1 to 80%; the cross-linking agent is oxidized dextran. Compared with the prior art, the injectable hydrogel possesses following advantages: the biocompatibility is high; in vivo in-suit formation, and in vivo degradation can be realized; it is beneficial for direct acting of drugs at diseased regions, and long lasting of drug effect; oxidized dextran is safe and notoxic as the cross-linking agent; environment pollution is reduced; the degradation products are amino acids and polysaccharides, and can be discharged out of human body via kidneys, so that the injectable hydrogel is harmless for human body.

Description

technical field [0001] The invention relates to the technical field of polysaccharide polymer materials, in particular to an injectable hydrogel and a preparation method thereof. Background technique [0002] Hydrogel material is a three-dimensional network structure formed by physical or chemical cross-linking. Hydrophilic residues combine with water molecules to connect water molecules inside the network, while hydrophobic residues swell with water. It has the advantages of high water content, strong water retention capacity, softness, certain viscoelasticity and shape, and good biocompatibility. Hydrogel materials have unique properties and are a new class of functional materials, which have become a research hotspot in the field of biomedical polymer materials. [0003] Injectable hydrogel exists in a solution state and can be mixed with drugs; when implanted in a solution state by injection, it can rapidly undergo a sol-gel phase transition and form a gel in situ; at t...

Claims

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Application Information

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IPC IPC(8): C08B37/08C08J3/24A61K9/06A61K47/36
CPCC08B37/003A61K9/0019A61K9/06A61K47/36C08J3/244C08J2305/08
Inventor 贺超良李自伊陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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