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Preparation method of silk fibroin material of composite nano-grade hydroxyapatite and application of silk fibroin material in repairing bone fracture parts

A technology of nano-hydroxyapatite and hydroxyapatite, which is applied in the fields of material science and clinical medicine, can solve problems such as inability to use, human discomfort, and corrosivity of bone tissue, and achieve simple methods, speed up repair, and promote proliferation and differentiation. Effect

Pending Publication Date: 2018-06-15
HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large amount of lactic acid produced by the degradation of polylactic acid is corrosive to bone tissue and causes discomfort to the human body, so it cannot be used for large-area fracture treatment.

Method used

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  • Preparation method of silk fibroin material of composite nano-grade hydroxyapatite and application of silk fibroin material in repairing bone fracture parts
  • Preparation method of silk fibroin material of composite nano-grade hydroxyapatite and application of silk fibroin material in repairing bone fracture parts
  • Preparation method of silk fibroin material of composite nano-grade hydroxyapatite and application of silk fibroin material in repairing bone fracture parts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Silkworm cocoons are degummed by steaming 0.005wt% sodium carbonate aqueous solution for 0.5h, and the degummed silk is dissolved in 9.3mol / L lithium bromide solution. The silk fibroin solution dissolved in lithium bromide was dialyzed in distilled water for 3 days and then freeze-dried to obtain a white foamy silk fibroin sample. The foamy silk fibroin is crushed and mixed into nano-sized hydroxyapatite (HA), and the mixture is made into a mixture in which HA accounts for 0% to 75% by weight of the total mass of HA and SF, and the mixture is dissolved in hexafluoroisopropanol to prepare A 25 wt% solution was obtained. Pour the silk fibroin / nanometer hydroxyapatite mixed solution into a pre-designed mold (the mold is made of polymer material polypropylene and is cylindrical, so as to make a cylindrical composite material at last), Then placed in methanol solvent for self-assembly regeneration. The methanol-treated material was air-dried and machined into bone screws. ...

Embodiment 2

[0033] The silk fibroin / nano-hydroxyapatite composites with different HA contents were made into cylinders with a diameter of 5 mm and a height of 1 cm, and their compressive properties were characterized. figure 2 As shown, we investigate the mechanical properties of the material through compression tests. figure 2 a shows the compressive stress-strain curve of the material, and the results show that the compressive strength and strain of SF-HA0 are 128.2 MPa and 47.9%, respectively. When the nHA content was 50wt%, the compressive strength of the composite reached 212.6MPa, while the compressive strain dropped to 33%. Further increasing the nHA content, the mechanical properties of the composites decreased sharply, indicating that nHA aggregated in the SF matrix. On the other hand, the elastic modulus of the material increases from 0.64GPa (SF / HA-0%) to 1.75GPa (SF / HA-50%); the toughness increases from 39.8MJ m -3 (SF / HA-0%) increased to 52.9MJ m -3 (SF / HA-50%), indicati...

Embodiment 3

[0035] The silk fibroin / nano-hydroxyapatite composite material obtained in Example 1 was cut into powder, and it was detected by Fourier transform infrared spectroscopy to study the mechanism of performance enhancement of silk fibroin after methanol treatment. image 3 Shown at 1658cm -1 、1545cm -1 and 1242cm -1 The absorption peaks at the positions belong to the characteristic absorption peaks of amide I, amide II and amide III of the silk fibroin molecule (Silk), indicating that its secondary structure is a random coil; after methanol regeneration, the three characteristic peaks are transferred to 1634cm -1 、1520cm -1 and 1230cm -1 , indicating that the silk protein transforms from random coils to β-sheets, which is beneficial to increase the strength of the material. In addition, in the infrared spectrum of the composite material 603cm -1 and 565cm -1 The characteristic absorption of corresponds to the phosphate O-P-O bending vibration, demonstrating the presence of...

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Abstract

The invention discloses a preparation method of a silk fibroin material of composite nano-grade hydroxyapatite and application of the silk fibroin material in repairing bone fracture parts. The preparation method comprises the following steps: uniformly dispersing nano-grade hydroxyapatite into silk fibroin in a certain ratio, dissolving the nano-grade hydroxyapatite with hexafluoroisopropanol, pouring a mixed solution into a columnar mold, soaking with methanol, performing self-assembling regeneration on a silk fibroin molecular chain so as to obtain a composite material which is excellent inmechanical strength, and finally manufacturing medicinal bone nails from the composite material by using a mechanical processing method. According to the characteristics that the material is good inbiocompatibility, excellent in mechanical property and good in in-vivo degradation controllability, the silk fibroin material can be applied to bone fracture fixation. A silk fibroin / nano-grade hydroxyapatite composite bone fracture internal fixation material with in-vivo degradation controllability can be prepared by using a method which is simple and feasible, high in finished product rate and free of toxicity, osteoporosis symptoms caused in the bone fracture repairing process can be effectively avoided, and because of the characteristic that the material does not need to be taken out through a second time of operation, bone fracture patients can be relieved from pain.

Description

technical field [0001] The invention relates to a preparation method of a silk fibroin material composited with nano-scale hydroxyapatite and its application in repairing fracture sites, belonging to the fields of material science and clinical medicine. Background technique [0002] Fracture refers to a complete or partial break in the continuity of the bone structure, which is more common in children and the elderly clinically, and is usually treated by surgical implantation of fracture fixation materials. Today's fracture internal fixation materials mainly include metal type, ceramic type and degradable polymer materials. Due to its good mechanical properties and low price, metal fracture internal fixation materials are commonly used in hospitals today, mainly including stainless steel and titanium alloys. However, due to the stress shielding effect of metal internal fixation materials, it is easy to induce the appearance of osteoporosis symptoms at the fracture site, res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/58
CPCA61L27/46A61L27/58A61L2300/214A61L2300/412A61L2300/604A61L2400/12A61L2430/02C08L89/00
Inventor 常春雨徐瑞张俐娜
Owner HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
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