Method for promoting in-vitro and in-vivo degradation and cell extension adherence of alginate-based 3D printed bioink

Abstract

The invention discloses a method for promoting in-vitro and in-vivo degradation and cell extension adherence of alginate-based 3D printed bioink. The method comprises the following steps: step 1, adopting alginate as an extracellular matrix main body skeleton and constructing celliferous 3D bio-printed bioink; step 2, mixing alginate lyase with alginate-based bioink; step 3, adding cell suspensioninto the obtained bioink, choosing print parameters and printing out a 3D printing block through a 3D bio-printer; step 4, regulating and controlling in-vitro and after-transplantation-in-vivo degradation time and cell extension adherence degree of the 3D printing block through adjusting the concentration of the alginate lyase according to actual demands. The method disclosed by the invention canpromote the degradation and cell extension adherence of the bioink with the alginate as the skeleton under in-vivo and in-vitro environments, and enables the alginate 3D printed bioink to be adaptedto more in-vivo and in-vitro experiments and be better applied to soft tissue repairing and transplantation.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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AI Technical Summary

Benefits of technology

This patented technology allows create tiny structures called algae within an organism's own structure during its growth period without causing harm themselves. By adding certain substances like alkaline earth metal salt into these alga particles, they gradually break down over time while still being able to keep their shape when placed under specific environmental factors such as temperature, pH level, etc., resulting in improved stability and performance compared to other methods previously described. Additionally, the algal material contains various types of polysaccharides which help promote blood clotting and wound healing processes through fibers. Overall, this new technique helps improve the properties of artificially made objects produced from alginate alone.

Problems solved by technology

Technologies described earlier include techniques like casting extracellular matrices onto substrates called stents, crushing necrosis cores from patients' bodies, culturing human epitheliutrophils, growing monoclinal tracts containing cells, injecting these cells into recipients, and delivering them back inside the patient's own body overcomes issues associated with current approaches including rejection responses due to inflammatory processes during surgery, poor wound healing mechanisms, reduced cell motility postoperatively, and potential negative side effectiveness related to prosthetic devices. Additionally, there may exist concerns about how effective therapy requires sufficient engraftment and growth of new ones undergoing natural metabolism. To address these challenges, research efforts aim towards developing improved solutions involving enzyme activation, cytokines release stimulation, matrix resin synthesis, and gene transfer technologies.

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