Surface-degradable three-dimensional (3D) printing bio-ink and 3D printing method

A 3D printing and bio-ink technology, applied in the biological field, can solve the problems that cells are difficult to obtain nutrients, 3D printed tissues are difficult to grow continuously, and metabolites cannot be discharged.

Active Publication Date: 2017-12-22
HANGZHOU MEDZONE BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In fact, it is precisely because of the above reasons that the existing 3D bioprinting technology can only print a very thin layer of tissue, because there are no nutrient channels such as blood vessels in the thicker tissue, it is difficult for the internal cells to obtain nutrients, and the metabolites cannot be discharged. Thicker 3D printed tissue struggles to sustain growth

Method used

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Examples

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Effect test

preparation example Construction

[0034] Further, the present invention also provides the preparation method of the 3D printing bio-ink described in Example 1, comprising the following steps: cross-linked hyaluronic acid with a mass fraction of 1%-5%, poly(N -isopropylacrylamide), mass fraction 3%-5% poly(N,N-diethylacrylamide)), mass fraction 3%-5% polymethyl vinyl ether, mass fraction 3%- 5% polyethylene glycol-polyethylene oxide (PEG-PEO) was blended with the remaining amount of deionized water, stirred for 30 minutes, and left to stand for 1 to 6 hours to make the hydrogel; the hydrogel was mixed with The biological cells are evenly mixed into the 3D printing bio-ink.

[0035] 3D printing bio-ink example 2

[0036] The aforementioned Example 1 provides a 3D printing bio-ink suitable for the present invention, the 3D printing bio-ink is a gel ink, the gel ink is composed of hydrogel and biological cells, and can be obtained by 3D printing without A model supported by brackets. Of course, those skilled in...

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Abstract

The invention relates to surface-degradable three-dimensional (3D) printing bio-ink and a 3D printing method. The 3D printing bio-ink consists of gel ink and support ink, wherein the gel ink is prepared from hydrogel and biological cells, wherein the hydrogel contains a poly(lactide-co-glycolide)-polyethylene glycol-poly(lactide-co-glycolide) (PLGA-PEG-PLGA) triblock copolymer with temperature response; the support ink is a poly(trimethylene carbonate)-poly(L-lactide) (PTMC-PLLA) copolymer having the surface degradation characteristic. By utilizing the temperature-sensitive and surface-degradable properties of the 3D printing bio-ink and aiming at the requirements of structural controllability and maintenance in an in vivo degradation process of a 3D bio-printing support, a double-layer printing structure similar to a coaxial tube is constructed by using a multi-component material gradient printing technology; after the 3D printing bio-ink and the 3D printing method are adopted, a model which contains biological cells and is supported by a controllably surface-degradable support can be obtained by printing and molding at a time, nutritional and metabolic channels of a porous structure can be also obtained, and a bigger and thicker structure can be promoted to be obtained by means of 3D printing.

Description

technical field [0001] The present invention relates to the field of biotechnology, especially the field of 3D bioprinting technology, in particular to a surface-degradable 3D printing bioink and a 3D printing method. Background technique [0002] 3D printing can quickly and efficiently manufacture personalized products, so it is gradually being introduced into the biomedical industry, with a view to using 3D printing technology for tissue and organ transplantation. "The Latest Research and Application of Bio-3D Printing" (Powder Metallurgy Industry, Volume 25, No. 4, August 2015, Zhang Hongbao, etc.) introduced the application of 3D bio-printing technology into different levels, which is quoted here as refer to. The basic method of higher-level 3D bioprinting is to inoculate living cells into a biocompatible and finally biodegradable scaffold, and then culture them in a bioreactor, so that the cells rely on the space formed by the scaffold to grow and generate the required...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09D11/30C09D11/38B29C64/112B33Y10/00B33Y70/00
CPCB33Y10/00B33Y70/00C09D11/30C09D11/38
Inventor 张洁裴明黎徐刚毅李杰甘志鹏王利群
Owner HANGZHOU MEDZONE BIO-TECH CO LTD
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