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Hyaluronic acid-methyl cellulose composite hydrogel as well as preparation and application thereof

A technology of methyl cellulose and composite hydrogel, which is applied in the field of biomedical materials and tissue engineering, can solve problems such as lack of binding and controlled release of growth factors, non-degradable or poor degradation performance, and biocompatibility problems. Prolong the retention time in vivo, improve the mechanical properties, and have good biocompatibility

Inactive Publication Date: 2016-02-17
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic nanoparticles and hydrophobic polymer micro-nanoparticles can effectively strengthen the hyaluronic acid hydrogel, but they cannot degrade or have poor degradation performance in the human body, and there are biocompatibility problems at the same time, and they have certain toxic effects on cells
In addition, the system also lacks the function of binding and controlling the release of growth factors

Method used

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  • Hyaluronic acid-methyl cellulose composite hydrogel as well as preparation and application thereof
  • Hyaluronic acid-methyl cellulose composite hydrogel as well as preparation and application thereof
  • Hyaluronic acid-methyl cellulose composite hydrogel as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Under magnetic stirring (speed 300rpm), add 50mL of pre-prepared artificial cerebrospinal fluid into the Erlenmeyer flask, heat it to 90°C in a constant temperature oil bath, add 4g of sodium chloride to it; after it is completely dissolved, Add 11g of methylcellulose and continue heating; after the methylcellulose powder is evenly dispersed and completely wetted, take out the Erlenmeyer flask, add 50mL of artificial cerebrospinal fluid, place it in an ice-salt bath at -4°C, and stir at 200rpm for 40min , to obtain a methylcellulose solution, which was refrigerated overnight at 4°C.

[0034] (2) After 24 hours, slowly add 1 g of hyaluronic acid powder to the methylcellulose solution, stir to dissolve, and add 2 mL of 0.4 g / L PEG solution dropwise; adjust the pH to about 7.4 with 1M NaOH solution, Stirring was continued for 2 hours to obtain a gel-like substance; the substance was refrigerated overnight at 4° C., and subjected to vacuum freeze-drying to obtain a hyal...

Embodiment 2

[0041] (1) Under magnetic stirring (speed 300rpm), add 50mL of pre-prepared artificial cerebrospinal fluid into the Erlenmeyer flask, heat it to 90°C in a constant temperature oil bath, add 4g of sodium chloride to it; after it is completely dissolved, Add 9g of methylcellulose and continue heating; after the methylcellulose powder is evenly dispersed and completely wetted, take out the Erlenmeyer flask, add 50mL of artificial cerebrospinal fluid, place it in an ice-salt bath at -4°C, and stir at 200rpm for 40min , to obtain a methylcellulose solution, which was refrigerated overnight at 4°C.

[0042] (2) After 24 hours, slowly add 1 g of hyaluronic acid powder to the methylcellulose solution, stir to dissolve, add 2 mL of 0.4 g / LPEG solution dropwise; adjust the pH to about 7.4 with 1M NaOH solution, and continue stirring After 2 hours, a gel-like substance was obtained; the substance was refrigerated overnight at 4° C., and subjected to vacuum freeze-drying to obtain a hyalu...

Embodiment 3

[0045] (1) Under magnetic stirring (speed 300rpm), add 50mL of pre-prepared artificial cerebrospinal fluid into the Erlenmeyer flask, heat it to 90°C in a constant temperature oil bath, add 4g of sodium chloride to it; after it is completely dissolved, Add 5g of methylcellulose and continue heating; after the methylcellulose powder is evenly dispersed and completely wetted, take out the Erlenmeyer flask, add 50mL of artificial cerebrospinal fluid, place it in an ice-salt bath at -4°C, and stir at 200rpm for 40min , to obtain a methylcellulose solution, which was refrigerated overnight at 4°C.

[0046] (2) After 24 hours, slowly add 1 g of hyaluronic acid powder to the methylcellulose solution, stir to dissolve, add 2 mL of 0.4 g / LPEG solution dropwise; adjust the pH to about 7.4 with 1M NaOH solution, and continue stirring After 2 hours, a gel-like substance was obtained; the substance was refrigerated overnight at 4° C., and subjected to vacuum freeze-drying to obtain a hyalu...

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Abstract

The invention belongs to the technical field of biomedical materials and tissue engineering technologies, and particularly relates to hyaluronic acid-methyl cellulose composite hydrogel as well as preparation and an application thereof. The hyaluronic acid-methyl cellulose composite hydrogel comprises methyl cellulose and hyaluronic acid in the mass ratio of (5-11):1 and adopts a crosslinking network structure, and the pore diameter ranges from 30 mu m to 100 mu m. The methyl cellulose with temperature-sensitive characteristic, good biocompatibility and small in-vivo degradation speed and hyaluronic acid molecules have a physical crosslinking reaction through polyethylene glycol, and the hyaluronic acid-methyl cellulose composite hydrogel is obtained. The composite hydrogel can be formed at the body temperature by controlling the concentration of the methyl cellulose and salt additives, the mechanical performance is improved, bioactive molecules can be controlled to be released, the in-vivo retention time is prolonged, regeneration and repair of soft tissue similar to nervous tissue are facilitated, and the hyaluronic acid-methyl cellulose composite hydrogel has wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and tissue engineering, and in particular relates to a hyaluronic acid-methylcellulose composite hydrogel and its preparation and application. Background technique [0002] Hyaluronic acid (HA) is a linear polysaccharide widely present in the extracellular matrix, containing two alternating units β-1,4-D-glucuronic acid and β-1,3-N-acetyl- D-glucosamine, is widely used for its advantages of non-antigenicity and biocompatibility. It promotes wound repair by reducing inflammation, tissue adhesions and scar formation. Hyaluronic acid also has unique rheological properties due to the fact that its long polymerized chains form random coils, and the molecules gel under the influence of entanglement. In existing studies, hyaluronic acid has been made into catheters or catheter fillers for the treatment of peripheral nerve and spinal cord injuries; it has been made into films for nerve repa...

Claims

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Application Information

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IPC IPC(8): C08L1/28C08L5/08C08J9/28C08J3/24C08J3/075A61L27/48A61L27/52A61L27/54A61L27/58
Inventor 蔡强卓凡露高琴胡堃王颖
Owner TSINGHUA UNIV
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