47 results about "Artificial cerebrospinal fluid" patented technology

A formulation is provided that includes a volume of an aqueous multivalent physiological ion solution compatible with cerebrospinal fluid containing at least one divalent cation of magnesium or calcium, and at least one anion of carbonate or phosphate, and having a pH between 6.5 and 8.0. A zwitterionic therapeutic agent other than baclofen is dissolved the solution to achieve higher concentration or ease of solution and / or storage relative to therapeutic saline solutions of the same agent. A process of delivering a zwitterionic therapeutic agent into a subject is provided that includes dissolving a therapeutic amount of the zwitterionic therapeutic agent in a volume of artificial cerebrospinal fluid to form a stable formulation. The solution is then administered to the subject using an intrathecal pump.

A high concentration baclofen solution is provided suitable for therapeutic use in a medical setting. A high concentration solution of baclofen in multivalent physiological ion solution such as artificial cerebrospinal fluid is provided with concentrations of baclofen of 10 mg / ml. Artificial cerebrospinal fluid is particularly advantageous as a baclofen solvent. A medical package is also provided for baclofen delivery to patients suffering from spasticity.

The invention discloses an artificial in-vitro preparation method of low molecular weight amyloid peptide oligomer and application, which is characterized in that the monomer of A beta polypeptide obtained with a gene recombination method is placed in a buffer system to react after dissolved by anhydrous dimethyl sulfoxide, thus naturally polymerizing to obtain oligomer. The method which obtains the monomer by gene recombination is economic and environmentally-friendly; when A beta serves as raw material to assemble amyloid peptide oligomer in vitro, a stable and even product is obtained by simulating in vivo physiological conditions and improving artificial cerebrospinal fluid components. The oligomer prepared with the method can serve as the standard reference for the detection and the therapy researches of various Alzheimer diseases, and can be applied in the diagnostic reagent and the therapy drug of the Alzheimer disease.

During neurosurgical procedures, surgeons are still using physiological saline to irrigate. Saline is harmful during neurosurgical procedures. An irrigating solution to replace the use of saline is provided. The irrigating solution includes magnesium, colloidal osmotic agent, insulin and ATP in artificial CSF.

The existence of the cerebrospinal fluid and the intracranial pressure contribute the susceptibility of the CNS to injuries. A lymph-like composition and method for treating brain and spinal cord injuries are provided. The lymph-like composition comprises Polypeptides, Insulin, Mg+ and ATP in an artificial cerebrospinal fluid. The method includes: a). Administering an agent to reduce the CSF production, b). Withdrawing a volume of cerebrospinal fluid, and c). Repeatedly injecting and withdrawing an effective amount of lymph-like composition through the subarachnoid space to wash the central nervous system tissue where protection is needed, and finally removing an effective amount of lymph-like composition to maintain a lower the intracranial pressure.

Described herein is the use of CSF, more particularly external CSF or CSF-like compositions for the treatment and prevention of different diseases. More particularly, the application provides for the administration of CSF to the intrathecal space or the cerebral ventricles of a patient to increase intracranial pressure and / or CSF flow.

The invention provides an in vitro brain slice nerve network oscillation recording system. The system comprises a brain slice recording tank, a storage tank, a gas supply device, a perfusion administration device, an electrode fixing and operating device, a temperature control device and a nerve network signal recording device; and the brain slice recording tank is a cuboid container, a groove is arranged in the container, two sides of the groove are provided with holes, an arc portion at the rear side of the groove is provided with a mixed oxygen saturated artificial cerebrospinal fluid input port, the mixed oxygen input port is connected with the gas supply device through a pipeline, the artificial cerebrospinal fluid cycle input port is connected with the perfusion administration device, and an artificial cerebrospinal fluid cycle pipeline is provided with a peristaltic pump. The in vitro brain slice nerve network oscillation recording dual oxygen supply system is obtained by modifying a traditional recording system with dual oxygen supply, a brain slice storage mode, temperature control and drainage devices, and has the advantages of long time storage of brain slices, maintenance of the biological activity of the brain slices, and induction of stable long-term nerve network oscillation activity.

A method of treating a glioma is disclosed. The method includes administering to a patient in need thereof a composition that includes a chemotherapy agent and artificial cerebrospinal fluid via convection enhanced delivery.

The invention discloses a method which is used for extracting and separating whole protein of rat hippocampus and is suitable for dimensional electrophoresis. The method comprises the following steps: (1) placing a rat in frozen artificial cerebrospinal fluid; (2) pouring liquid nitrogen in a mortar for precooling before grinding; (3) standing turbid liquid at room temperature; (4) subpackaging liquid supernatant; (5) adding RB ( rehydration buffer) into protein conglomeration; (6) making a standard curve by 5, 10, 15, 20, 25, 30 and 35 micrograms of BSA (Bull Serum Albumin); (7) loading analysis gel, namely silver staining, with the quantity shown in the specification; (8) taking out a dried gel strip and rewarming at room temperature; (9) adding coverage oil into an isoelectric focusing disk; (10) performing isoelectric focusing treatment; (11) rewarming the treated gel strip; (12) preparing low melting agarose from upper tank liquid; and (13) carrying out coomassie blue staining on the gel and drawing. The method is easy to implement and is suitable for extracting of rat hippocampus tissue protein samples, the operation is simple and convenient, and a dimensional electrophoretogram with more protein spots, clear horizontal stripes and good repeatability is obtained.

The invention discloses an extensible flexible electrode transfer method based on an elastic seal containing a fluid channel. The method comprises the following steps: firstly pressing the elastic seal on an extensible flexible electrode, and injecting a water-soluble rapid degradation material solution into the fluid channel; then heating and drying the solution, and enabling the extensible flexible electrode to be lifted up from a glass substrate by the elastic seal through adhesion; then, transferring the elastic seal with the extensible flexible electrode attached to the bottom face to the position above a target brain area through a micro-motion platform, injecting artificial cerebrospinal fluid into the fluid channel, and gradually dissolving a degradation material; finally, after the degradable material is completely dissolved, separating the elastic seal from the extensible flexible electrode so as to enable the extensible flexible electrode to be stably attached to the surface of the cerebral cortex. The method has very important practical value and innovative significance for accurately acquiring stable electrocorticogram signals of target brain area coordinates, and can effectively solve the problems that attaching operation difficulty of an discretized extensible flexible electrode of the electrode point at present is large and the relative position of the electrode point is not easy to control.

A chemotherapy agent or a pharmaceutical composition including chemotherapy agent and artificial cerebrospinal fluid, wherein the chemotherapy agent at a concentration of between 0.01 mg / ml and 0.7 mg / ml for use in the treatment of brain cancer and a method of treating a glioma including administering a chemotherapy agent via convection enhanced delivery

This invention provides a method for identifying a small molecule as an antidepressant, a method for identifying a small molecule as an anxiolytic, and a method for identifying a small molecule as able to increase dendritic arborization, decrease expression of an immaturity marker, increase expression of a maturity marker, or enhance artificial cerebrospinal fluid-type long-term potentiation in central nervous system. This invention also provides a transgenic mouse model for SSRI-non-responders.

The invention discloses a kit and a method for primate neuron separation, and relates to the technical field of biological cells. According to the kit disclosed by the invention, the formulas of the artificial cerebrospinal fluid and the brain tissue repairing fluid are optimized, and the inhibitor mixture is added into the artificial cerebrospinal fluid, so that the survival rate of neurons is increased, the neurotoxicity is reduced, and the kit is more suitable for separating the brain cells of primates. According to the kit, the single neuron of the primate animal is successfully separated for the first time, survival of more than 90% of the neuron can be guaranteed, cell bodies, dendrites, axons, other protrusions and the like of the neuron can be reserved, almost all information of the single neuron of the primate animal can be detected, heterogeneity of different neuron can be deeply known, and more comprehensive and complete information of neurons is explored.

The invention discloses a cerebrospinal fluid circulation assisting apparatus, which comprises an artificial cerebrospinal fluid import catheter, a cerebrospinal fluid export catheter and an infusion device, wherein a liquid outlet end of the artificial cerebrospinal fluid import catheter and a liquid inlet end of the cerebrospinal fluid export catheter are arranged in a cerebral ventricle of a human body, and the liquid outlet end is located at the back side of the liquid inlet end; a liquid outlet of the cerebrospinal fluid export catheter is arranged in an abdominal cavity of the human body; the cerebrospinal fluid export catheter is provided with a one-way liquid storage bag and a pressure control valve; the infusion device comprises an automatic control infusion pump and an infusion bag; the automatic control infusion pump comprises a shell and a microprocessor located in the shell; a cavity, which is used for accommodating the infusion bag, is formed in the shell; a liquid conveying mechanism, which is used for controlling artificial cerebrospinal fluid in the infusion bag to be inputted into the cerebral ventricle of the human body according to a preset quantity, is arranged in the cavity; the liquid conveying mechanism is connected to the microprocessor; a liquid discharging needle of the infusion bag is connected to an L-shaped nondestructive needle by virtue of a connecting tube; and the L-shaped nondestructive needle is inserted on an infusion seat which is connected to the liquid inlet of the artificial cerebrospinal fluid import catheter and is buried in a subcutaneous breast when the artificial cerebrospinal fluid is replenished.

The invention provides a multifunctional bath for in-vitro electrophysiological recording, and relates to the technical field of incubation systems, the multifunctional bath comprises a box body, a water tank, a control box and a bath, the water tank, the control box and the bath are sequentially connected in the box body, and internal circulation of the bath is realized by connecting the water tank with the bath through a water inlet pipe and a water outlet pipe; the control box is in control connection with a heating rod, an oxygen probe, a temperature probe and a PH probe in the water tank. According to the invention, temperature, PH and oxygen of the artificial cerebrospinal fluid in an incubation tank are detected in real time through the temperature sensor, the PH sensor and the oxygen sensor, so that the experimental environment is detected in real time, and adjustment is convenient. Compared with a traditional incubation system, the incubation system provided by the invention can save space, six mouse brain slices can be incubated at the same time, and the experiment efficiency is improved.

The invention provides new application of colloidal gold to preparation of an artificial cerebrospinal fluid reagent. The colloidal gold comprises colloidal gold particles, wherein the particle size range is 5 to 25 [mu]m, the average particle size is 13.4+ / -3.6 [mu]m, the colloidal gold concentration is 150 to 400 mg / L, and the average concentration is 232.4+ / -38.3 mg / L. The colloid osmotic pressure and the cell environment of human cerebrospinal fluid can be simulated, and novel artificial cerebrospinal fluid according with the physiological characteristics is created.

The invention relates to the field of lumbar vertebra purification equipment, in particular to a disposable pipeline mounting system for continuous purification treatment of artificial cerebrospinal fluid. The disposable pipeline mounting system comprises a storage device, an infusion volume metering device, a fluid outlet volume metering device, a waste fluid bag and a double-cavity catheter; thestorage device comprises a storage bag for storing the artificial cerebrospinal fluid, a storage fluid connection tube connected with a fluid outlet of the storage bag, and a filter arranged on the storage fluid connection tube and used for filtering the artificial cerebrospinal fluid in the storage fluid connection tube; and one end of a first fluid outlet tube communicates with a fluid inlet ofthe double-cavity catheter, the other end of the first fluid outlet tube communicates with a fluid outlet of a first injection pump through a first fluid outlet one-way valve, and thus the artificialcerebrospinal fluid flows to the double-cavity catheter from the first injection pump. The disposable pipeline mounting system can be matched with existing special artificial cerebrospinal fluid continuous purification equipment, and is applied to in-vitro purification treatment of cerebrospinal fluid of central nervous system diseases causing composition, character and circulatory dynamics changes of the artificial cerebrospinal fluid.

The present invention provides methods for the treatment of a central nervous system (CNS) tumor in a subject comprising administering and IL-4 targeted cargo protein formulated in an artificial cerebral spinal fluid formulation. The present invention also provides formulations and methods for administration along with a surrogate tracer for monitoring.

The invention relates to a method for simply preparing DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) microscopic particles and marking neurons, wherein the method for simply preparing DiI microscopic particles comprises the following steps of: dissolving DiI particles in absolute ethanol or dimethyl sulfoxide to form a DiI solution of which the mass volume concentration is 0.01-0.1g / ml; adding the DiI solution of which the mass volume concentration is 0.01-0.1g / ml into artificial cerebrospinal fluid or phosphate buffer of which the concentration is 0.1mol / L; and enabling the DiI solution to quickly float on the artificial cerebrospinal fluid or phosphate buffer in a film form, and moving and diffusing all around to precipitate DiI microscopic particles of which the particle sizes are 3-10 micrometers. The method provided by the invention is simple in operation and low in cost.

The present invention provides a method for producing a cyclodextrin-panobinostat adduct, comprising: a) providing a first aqueous solution comprising a buffering agent, the solution having a pH in the range 2.0 to 4.0; b) dissolving panobinostat in said first aqueous solution to provide a second aqueous solution; and c) mixing said second aqueous solution comprising panobinostat with a third aqueous solution comprising a cyclodextrin to form a fourth aqueous solution comprising a cyclodextrin-panobinostat adduct. Also provided is an artificial cerebrospinal fluid (CSF) solution comprising the cyclodextrin-panobinostat adduct and medical uses of the cyclodextrin-panobinostat adduct, including in the treatment of brain tumours.

A high concentration baclofen solution is provided suitable for therapeutic use in a medical setting. A high concentration solution of baclofen in multivalent physiological ion solution such as artificial cerebrospinal fluid is provided with concentrations of baclofen of 10 mg / ml. Artificial cerebrospinal fluid is particularly advantageous as a baclofen solvent. A medical package is also provided for baclofen delivery to patients suffering from spasticity.

The present invention relates to a method and a device for determining the hydro-dynamic properties of the fluid system surrounding the brain and the spinal cord, the so called cerebrospinal fluid system, whereby the method comprises continuous pressure measurement through a fluid contact passage and active infusion of artificial cerebrospinal fluid through another fluid contact passage to a number of pressure-flow levels, and analysis of the connections between the measured pressures and flows. The method uses an adaptive way of procedure which on each pressure-flow level takes account for the measure time and the patient's fluctuations in physiologic signals for calculating in real time when the relation between the measure time and measure accuracy in pressure and flow on the actual pressure-flow level is sufficient and at an optimum, whereupon the investigation or examination is automatically proceeding to the next level according to a predetermined protocol. In this way, the method gives rise to an investigation with measurements having sufficient pressure and flow information on each level, which forms a base for determination in a correct manner, with an uncertainty estimate, of the patient's hydrodynamic parameters. The device comprises a hose pump (1) for infusion of artificial cerebrospinal fluid in a bottle or bag (2), a standardized hose set including a pump hose (3) and pressure transducers (4) for continuous registration of the intracranial pressure, an invasive contact object (5) for creating fluid contact with the cerebrospinal fluid system and consisting of needles or a catheter, as well as a computer (12) with software for computerized collection and analysis as well as control of pump speed. The device uses a computerized implementation of the above-mentioned adaptive method in order to systematically and safely carry through a standardized protocol which generates pressure and flow information which the software of the measure system then uses for determining in real time and with an uncertainty estimate, the hydrodynamic parameters of the system.

The invention discloses a method for evaluating signal recording performance of a nerve microelectrode. The method comprises the following steps: putting the nerve microelectrode into artificial cerebrospinal fluid, and carrying out noise and signal level detection; carrying out surface cleaning on the nerve microelectrode, drying the nerve microelectrode, implanting the nerve microelectrode intoa specific brain area of a subject, and recording a local field potential of the subject, wherein data is the sum of the detected noise and signal levels of the nerve microelectrode; preprocessing a collected electric physiological signal so as to obtain a time domain waveform, and superposing and averaging detection signals so as to respectively obtain the noise level and the signal level; and quantizing a detection signal to noise ratio of the nerve microelectrode, so as to finish the evaluation of the performance of the nerve microelectrode. The invention relates to recording of a spontaneous potential of the nerve microelectrode in the electric physiological signal of an electroencephalogram nerve. The method can be applied to the evaluation of the performance of the nerve microelectrode and can be applied to comparison on the quality of detection signals of different nerve microelectrodes after the nerve microelectrodes are optimized or modified.

The invention discloses magnesium-rich artificial cerebrospinal fluid, and a preparation method and applications thereof. Used raw materials are clinical intravenous medications, so that the raw materials are easy to obtain and low in price; the magnesium-rich artificial cerebrospinal fluid is simple in preparation technology, and complex sterilization processes are not needed; the magnesium-richartificial cerebrospinal fluid is time- and labor-saving in preparation and can be used while being prepared; and through animal experiments and small-scale clinical studies, the safety and effectiveness of the magnesium-rich artificial cerebrospinal fluid can be verified. In summary, obtained drugs have the advantages of being simple in preparation, economical, practical, safe and reliable; and new thoughts and directions can be provided for clinical work.

The invention relates to a packaging device for artificial cerebrospinal fluid. An infusion bag is internally and sequentially provided with a foaming chamber, a liquid chamber B and a liquid chamberA from top to bottom; the liquid chamber B stores an alkaline electrolyte solution containing sodium bicarbonate, and the liquid chamber A is acid and contains glucose; partition belts which can be opened at a time are arranged between the foaming stick chamber and the liquid chamber B and between the liquid chamber B and the liquid chamber A respectively, and the partition belts make the foamingstick chamber, the liquid chamber B and the liquid chamber A isolated. A foaming stick is fixed on the inner wall of an infusion bag by gluing through a shaft rod, one end of the shaft rod is horizontally embedded into a vent pipe, and the other end of the shaft rod is solid. The other end of the vent pipe penetrates through the infusion bag, extends outward to form an exposed pipeline and is sequentially provided with a one-way check valve, a closed pipe clamp and a pipe-end nut, mixed gas is introduced through the vent pipe, and the bottom of the infusion bag is provided with a sealed air outlet / dosing hole and an infusion hole.

The invention relates to the field of lumbar vertebra purification equipment, in particular to a disposable pipeline mounting system for continuous purification treatment of artificial cerebrospinal fluid. The disposable pipeline mounting system comprises a storage device, an infusion volume metering device, a fluid outlet volume metering device, a waste fluid bag and a double-cavity catheter; thestorage device comprises a storage bag for storing the artificial cerebrospinal fluid, a storage fluid connection tube connected with a fluid outlet of the storage bag, and a filter arranged on the storage fluid connection tube and used for filtering the artificial cerebrospinal fluid in the storage fluid connection tube; and one end of a first fluid outlet tube communicates with a fluid inlet ofthe double-cavity catheter, the other end of the first fluid outlet tube communicates with a fluid outlet of a first injection pump through a first fluid outlet one-way valve, and thus the artificialcerebrospinal fluid flows to the double-cavity catheter from the first injection pump. The disposable pipeline mounting system can be matched with existing special artificial cerebrospinal fluid continuous purification equipment, and is applied to in-vitro purification treatment of cerebrospinal fluid of central nervous system diseases causing composition, character and circulatory dynamics changes of the artificial cerebrospinal fluid.

A method and apparatus for preventing brain death by using rapid and safe cooling of the brain is disclosed. A needle / cannula allows cooled artificial cerebrospinal fluid (aCSF) to flow around the brain by accessing a cerebellar medullary pool having access through the patient's neck with a specially designed needle / cannula, accessing the cerebellar medullary pool through the patient's neck, and circulating the cooled artificial cerebrospinal fluid (aCSF) around the space within the brain and in the subarachnoid space around the brain. The aCSF exits from an opening in the skull in which the temperature / pressure sensor is placed. The data is sent to a computer-controlled motorized system that pumps the cooled aCSF to a needle / cannula placed in the cerebellar medulla oblongata cell. Pumping of the aCSF is controlled to maintain a predetermined temperature and / or pressure of the outlet aCSF.

The invention relates to a method for simply preparing DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) microscopic particles and marking neurons, wherein the method for simply preparing DiI microscopic particles comprises the following steps of: dissolving DiI particles in absolute ethanol or dimethyl sulfoxide to form a DiI solution of which the mass volume concentration is 0.01-0.1g / mL; adding the DiI solution of which the mass volume concentration is 0.01-0.1g / ml into artificial cerebrospinal fluid or phosphate buffer of which the concentration is 0.1mol / L; and enabling the DiI solution to quickly float on the artificial cerebrospinal fluid or phosphate buffer in a film form, and moving and diffusing all around to precipitate DiI microscopic particles of which the particle sizes are 3-10 micrometers. The method provided by the invention is simple in operation and low in cost.