In vivo brain slice nerve network oscillation recording system and recording method thereof

Abstract

The invention provides an in vitro brain slice nerve network oscillation recording system. The system comprises a brain slice recording tank, a storage tank, a gas supply device, a perfusion administration device, an electrode fixing and operating device, a temperature control device and a nerve network signal recording device; and the brain slice recording tank is a cuboid container, a groove is arranged in the container, two sides of the groove are provided with holes, an arc portion at the rear side of the groove is provided with a mixed oxygen saturated artificial cerebrospinal fluid input port, the mixed oxygen input port is connected with the gas supply device through a pipeline, the artificial cerebrospinal fluid cycle input port is connected with the perfusion administration device, and an artificial cerebrospinal fluid cycle pipeline is provided with a peristaltic pump. The in vitro brain slice nerve network oscillation recording dual oxygen supply system is obtained by modifying a traditional recording system with dual oxygen supply, a brain slice storage mode, temperature control and drainage devices, and has the advantages of long time storage of brain slices, maintenance of the biological activity of the brain slices, and induction of stable long-term nerve network oscillation activity.

Description

technical field [0001] The invention belongs to the field of partial preservation of human or animal tissues, and in particular relates to a test device for isolated brain slices and an application method thereof. Background technique [0002] Gamma neural oscillation is a 30-80Hz synchronous signal that exists in many areas of the human and animal brains. Studying neural oscillations can understand the neural characteristics of animal and human cognition, learning, memory, excitement, and arousal, and it is a hot spot in medical and psychological research. [0003] Currently, known isolated neural network oscillation techniques include brain slice recording devices, recording tanks, gas supply devices, perfusion drug delivery devices, lighting and temperature control devices, electrode fixation and manipulation devices, neural network signal recording devices, shielding and Grounding device and system shockproof device. After the brain slices are prepared, put them into t...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of low brain slice activity, short duration of induced neural network oscillations, and low energy spectrum in the traditional neural network oscillation recording system, the present invention provides an improved isolated brain slice neural network oscillation recording system. The recording system has made many improvements and innovations to the traditional recording system, which can store the brain slices for a long time and maintain their biological activity, and can induce stable long-term neural network oscillation activities, which can better meet the requirements of the experiment. , a lot of brain slices can be cut out from experimental animals and tissues at the same time, and other brain slices are placed in the brain slice storage tank. Because this storage method can keep the brain slices in good condition for more than 12 hours, it reduces the need for related experimental animals and tissues and be able to complete experiments that could not be completed due to the short period of time when the state of the brain slices was maintained well

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