55results about How to "Meet the requirements of the experiment" patented technology

The invention provides a stabilized-pressure oil supply system of a high-pressure direct-injection injector and a control method thereof. The stabilized-pressure oil supply system mainly comprises a high-pressure nitrogen cylinder, a pressure reducer, a step motor, a pressure sensor A, a two-position three-way electromagnetic valve A, an accumulator, a two-position three-way electromagnetic valve B, a pressure sensor B, an injector, a check valve, a fuel pump, an oil tank, a controller and a display screen. The stabilized-pressure oil supply system can automatically regulate the injection pressure of the injector of the stabilized-pressure oil supply system to a set injection pressure through the controller of the stabilized-pressure oil supply system according to the pressure parameter set on the display screen and can reduce the oil pressure fluctuation in the injection of the injector. The stabilized-pressure oil supply system has an accumulator oil supply path, so the fuel supply to the accumulator can be realized without detaching the pipe.

The invention belongs to the technical field of wind tunnel tests, and in particular relates to a device and method for measuring the flow of a flap gap based on a PIV method. The method comprises thesteps of emitting a laser sheet from an emitting end of a laser, and dividing the laser sheet into two laser sheets through a light path device; coarsely tuning reflectors in the light path device and making the two laser sheets radiating respectively from two directions into a flap gap; and finely tuning the reflectors and making the two laser sheets overlap, that is, completing the constructionof a light path. The device includes a beam splitting cube, reflectors, and an optical clamping member. The device also includes a PIV unit. The device is simple and practical in structural principle, well solves the problem of light path limitation on the PIV shooting of a flow field like a flap gap environment, and greatly improves the efficiency of PIV shooting.

The invention provides a method for separating and culturing pituitary gland cells of a rat. The method comprises steps as follows: (1), an 8d male SD rat is selected and subjected to anesthesia execution; (2), the anesthetized rat is fixed on a board with the ventral side facing upwards, the pituitary gland of the rat is taken out under the sterile condition, put in a pre-cooled PBS (poly(butylene succinate)) liquid and washed repeatedly, bloodstain is removed, and pituitary gland envelope is removed; (3), the pituitary gland is cut into 1 mm*1 mm fragments by ophthalmic scissors in the pre-cooled PBS liquid under the sterile condition; (4), pre-heated III type collagenase is combined with neutral enzyme for mixed digestion; (5), the digestion is stopped, filtering is performed, a filtrate is collected and centrifuged, a supernatant is abandoned, a culture medium is added for resuspension, and the operation is repeated three times; (6), a 24-hole culture plate is inoculated with cells for culture; (7), the cells cultured for 7d are subjected to trypsin digestion and then are purified by a screen again, a supernatant is abandoned after centrifugation, the cells are resuspended, and the treated culture plate is inoculated with the cells; (8), the cells are subjected to cell immunofluorescence identification. The method for separating and culturing the pituitary gland cells of the rat is simple to operate, the obtained pituitary gland cells are high in survival rate and good in activity, can be used for establishing a cell experimental model in vitro and meet the requirement of pituitary gland cell experiments.

The invention relates to a method of extracting high-quality DNA (Deoxyribonucleic Acid) of a fin product and belongs to the field of molecular biology associated research. The method comprises the following steps: (1) pre-treating a fin product sample, namely softening and smashing fins in a weak base solution; (2) splitting the pre-treated fin product sample by a CTAB (Cetyltrimethyl Ammonium Bromide) splitting solution; (3) extracting the DNA by balanced phenol and chloroform-isoamylol in steps; (4) adding a high-saline solution of isopropanol doubled in volume into an aqueous phase containing the DNA to obtain a DNA precipitate; washing the DNA precipitate by an ethanol solution with the volume fraction of 75%, fully drying, and then, dissolving the DNA precipitate and storing. The method of extracting the DNA of the fin product provided by the invention can be used for conveniently and efficiently obtaining the high-quality DNA of a fin tissue so as to avoid pollution by polysaccharide, collagen and other impurities.

The invention provides a method for isolating and culturing rat Schwann cells, comprising the following steps: (1) disinfecting tissue processing instruments; (2) selecting 2-3 week old male SD rats, killing them by neck dislocation, and using ophthalmic scissors Cut off the hair on the legs of rats, cut off the sciatic nerve, put the tissue into pre-cooled sterile PBS solution containing double antibodies and wash several times to remove blood stains and adventitia; (3) Mince the tissue, and use preheated type II collagen (4) Terminate the digestion, centrifuge, discard the supernatant, add culture medium and resuspend; (5) Inoculate the tissue block into a 6-well culture plate for culture by adherent method; (6) Incubate for 1-2 hours, Placed in a 37°C, 5% CO2 environment for culture; (7) Cell subculture and purification; (8) Cell morphology observation and identification. The method for separating and culturing rat Schwann cells provided by the present invention is simple and easy to operate, stable and efficient, and the obtained Schwann cells have high vigor, which can be used to establish model cells for in vitro experiments and meet the requirements of rat Schwann cell experiments .

The invention provides a pair of environment-friendly chopsticks, which is prepared by uniformly mixing and drying reed haulm powder, xanthan gum solution and guar gum solution. The invention further provides a preparation method for the pair of environment-friendly chopsticks. The invention provides the pair of reed chopsticks which is brand-new and environment-friendly, the use is safe and convenient, environment pollution is reduced, and ecological balance is kept.

The invention provides a separation and culture method of primary human retina Muller cells. The separation and culture method comprises the following steps: (1) a normal eyeball surgically excised from a corneal donor is taken out aseptically; (2) under a dissecting microscope, retina tissue is detached aseptically; (3) mixed collagenase is added for digestion; (4) digestion is terminated, filtering, centrifuging and washing are conducted, re-suspending is conducted through a complete medium, and the complete medium is inoculated into a culture bottle; (5) the culture bottle is placed into a5% CO2 incubator at 37 DEG C to be cultured; (6) cell passage and purification are conducted; and (7) the cell morphology is observed and identified. According to the separation and culture method ofthe human retina Muller cells, operation is easy, stability and high efficiency are achieved, and a good experimental material is provided for further studying a retinopathy damage generation mechanism.

The invention provides a human primary gastric cancer cell separation and culture method. The method comprises the following steps of (1) disinfecting a tissue processing device; (2) using a pair of operating scissors for separating a gastric cancer tissue, placing the tissue into a precooled sterile PBS liquid containing double antibodies, and washing for multiple times to remove bloodstains andnecrotic tissues; (3) cutting up the tissues, and using preheated IV type collagenase and trypsin for digesting respectively; (4) terminating digestion, centrifuging, and removing a supernatant; (5) after carrying out cell counting, inoculating into a coated culture flask; (6) culturing at 37 DEG C in an environment with 5 percent of CO2; (7) purifying the cells; (8) subculturing the tissues; (9)observing cell morphology; (10) determining cell survival rate; (11) carrying out immunological identification. The invention provides the human primary gastric cancer cell separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human gastric cancer cell primary separation and culture method, and a reliable cell resource is provided for experiments.

The invention relates to the science popularization field for standing wave tests, and more particularly, to a science popularization device for sound wave visualization. The device comprises a base, a horizontally arranged rolling cylinder, a driving motor to drive the rolling cylinder to rotate, an elastic string group, and a lifting and lowering support to drive the elastic string group to rise and fall. The elastic string group comprises a plurality of elastic strings arranged horizontally right above the rolling cylinder; and the elastic strings are perpendicular to the axis of the rolling cylinder. The lifting and lowering support comprises a first lifting and lowering frame and a second lifting and lowering frame arranged at two ends of the elastic string group. The cylindrical face of the rolling cylinder is provided with strips which are arranged alternately and whose colors are black and white. According to the invention, based on the height of an observer and through the rotation of a first rotating rod and a second rotating rod to adjust the distance from the elastic strings to the rolling cylinder as well as for the strips under the rotation of the rolling cylinder, it is possible to notice the wave fluctuations clearly. The rotation of a second threaded pipe makes the second threaded pipe generate certain displacement away from a first mounting plate with regard to a first threaded pipe so that the elastic strings are stretched to some length to ensure that each elastic string is tight and that the elasticity of the elastic strings meets the testing requirement.

The invention belongs to a laboratory ultrasonic detection device, in particular to a device capable of setting the collection angle in all directions during the ultrasonic data collection process. The universal positioning device is arranged on the three-dimensional positioning device; the universal positioning device is connected with the host through a communication port; the three-dimensional positioning device includes a universal positioning device and a transducer; the universal positioning device is used to control the transducer Rotate for continuous data acquisition. The invention overcomes the energy inconsistency and arrangement limitation caused by the directivity of the transducer in the existing experimental system, improves the simulation degree of the seismic physics experiment system, expands the experimental research content, improves the productivity, and ensures the accuracy of the experimental data. Reliability and practicability meet the experimental requirements of oil and gas exploration and development. The purpose of high efficiency, quickness, flexibility, precision, reliability and strong practicability is achieved.

The invention relates to a high-speed WFOV (wide field of view) CARS (coherent anti-stokes raman scattering) microscope system and a high-speed WFOV CARS microscope method. In the invention, pumping laser and Stokes light laser which are totally coincident in the aspects of space and time are subjected to weak convergence, so that a sample generates a CARS signal; and the CARS signal enters a CCD (Charge Coupled Device) camera through an optical filter and a cylindrical lens so as to obtain a clear CARS image. The invention utilizes the CARS signal to image and relates to an imaging technology based on the vibration characteristic of energy level inside molecules. The high-speed WFOV CARS microscope system and the high-speed WFOV CARS microscope method can be used for detecting chemical compositions of the sample and can be used for carrying out imaging on a single cell, even a single organelle. The requirements of most of biological experiments are totally met. The technical problems of low imaging speed, series photic damage to living biological tissues and the like of the existing CARS microtechnique are solved. Compared with the common fluorescence microscopy, the high-speed WFOV CARS microscope system and the high-speed WFOV CARS microscope method have the advantages that an external fluorescent probe does not need to be used, and influence on the molecular structure of the sample cannot be caused.

The invention discloses a kit for detecting cow milk content in a goat milk product and application of the kit, and belongs to the technical field of food safety. The kit comprises a specific primer and a probe for internal comparison genes as well as a primer and a probe for specific genes of a cow, wherein the specific primer for the internal comparison genes is designed and obtained after comparing the homologous sequences of 12SrRNA of the cow and a goat; the primer for the specific genes of the cow is designed for the partial nucleotide sequence of the 12SrRNA of the cow. The invention further provides a method for detecting the cow milk content in the goat milk product. The method comprises the following steps: extracting the DNA of a sample; utilizing the primer and probe for internal comparison as well as the primer and probe for the specific genes to carry out an FQ-PCR amplification reaction; quantitatively detecting the cow milk content in the goat milk product. The method is accurate, reliable, high in specificity, low in detection limit, high in reproducibility and suitable for rapid and quantitative detecting of the cow milk content in the goat milk product.