The invention discloses a
simulation three-dimensional
incubator and a culture method for
mouth mucosa epithelial cells and applications of the
incubator and the culture method. The
incubator comprises a single or a plurality of basic structural units, wherein each basic
structural unit consists of an inner container and an outer container which are tightly nested with each other, and are prepared from an organic material for
cell culture; and the bottom of the inner container consists of a permeable supporting film, and a certain gap is formed between the bottom of the inner container and the bottom of the outer container. The incubator takes the factors of
nutrient supply of the
mouth mucosa epithelial cells, blood vessels and
blood vessel endothelial cells into account, and the growing environment of the
mouth mucosa epithelial cells in the incubator is close to that in a
human body, so that the incubator can be used for physiological, pharmacological, toxicological and micro-ecological studies of mouth mucosas, and can also be used for constructing human
tissue engineering mouth mucosas and repairing and reconstructing mucosal
coloboma. The new technology of the
simulation three-dimensional incubator promotes an
in vitro culture model of a mouth mucosa to a new height, so that related studies are closer to clinical practices.