Separation and culture method for primary pig liver cells
A culture method and technology of pig liver, applied in the field of cell culture of modern biotechnology, can solve the problems of poor activity and low number of liver cells, and achieve the effects of stable quality, high cell viability and good activity.
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[0024] The terms used in the present invention generally have the meanings commonly understood by ordinary skills in the art unless otherwise specified.
[0025] The present invention will be described in detail below with reference to the drawings and examples. The protection content of the present invention is not limited to the following embodiments.
[0026] In the following embodiments, various processes and methods that are not described in detail are conventional methods known in the art.
[0027] The experimental instruments and reagents used in this experiment are as follows:
[0028] A set of surgical instruments, CCK-8 cell counting box (Sigma, USA), inverted microscope (XDS-1A, Shanghai), fluorescence microscope (Leica, USA), cryogenic centrifuge (TD24B-WS, Shanghai), ultra-low temperature refrigerator ( Zhongke Meiling), pipette gun (Eppendorf, USA), electronic analytical balance (Sartorius, USA), ultra-clean workbench (HJ-CJ-1D, Shanghai), CO 2 Cell incubator (SANYO MCO-...
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