42 results about "Amylobarbitone sodium" patented technology

The present invention provides one composite medicine for treating chronic heart failure. The composite medicine has the functions of invigorating vital energy, dredging meridian, promoting blood circulation and diuresis. The medicine composition has 11 kinds of Chinese medicinal materials including astragalus root or white atractylodes rhizome, mankshood, ginseng or Dangshen, red sage, pepperweed seed, etc. The pharmacological test shows that the capsule of the present invention has obvious improving effects to heart failure of dog caused by Pentobarbital sodium.

The invention discloses a lactobacillus brevis capable of relieving anxiety and improving sleep and application of the lactobacillus brevis, and belongs to the technical field of microorganisms. The lactobacillus brevis GDMCC No.60605 can survive well in simulated gastrointestinal tract and has excellent cell adhesion and GABA production abilities. In an open-field experiment, the frequency of mice entering a central area can be significantly increased, and the time of the mice staying n the open-field central area is prolonged without affecting the total movement distance of the mice. In a high plus maze test, the number of times of the mice entering open arms can be significantly increased, the pentobarbital sodium induced sleep time can be prolonged, the pentobarbital sodium induced sleep latency can be shortened, the intestinal flora can be adjusted, and the relative abundance of Allobaculum in the intestines of the mice is significantly increased. The lactobacillus brevis GDMCC No.60605 is used for preparing a pharmaceutical composition and a fermented food capable of relieving anxiety and improving sleep, and has a very wide application prospect.

The invention provides a method for separating and cultivating rat hepatocytes. A rat is anaesthetized by Nembutal and is given sodium heparin for anticoagulation; portal catheterization is carried out; two-step reperfusion is carried out for perfusion; a digestive mature liver is taken off; hepatocytes are collected in Hanks liquid containing 1 percent of bovine serum albumin (BSA); hepatocyte suspension is screened by a screen stencil; after the filtrate is centrifugated and deposited, the deposit is collected in Leibovitz's-15(l-15) complete medium; the density of hepatocyte is adjusted to 3 to 6 *10<5>/ml and inoculated on a culture plate coated with rat tail collagen in the environment of temperature of 37 DEG C and 5 percent of CO2; and unattached and dead hepatocytes are removed by changing medium after 4 hours of inoculation, and the hepatocytes can be used for experiment after 20 hours of cultivation. The method is easy, simple and stable, has low cost and high efficiency, can obtain high-activity hepatocytes, is easy to attach, and can be used for pharmacological and toxicity experiments and popularized in common laboratories.

The invention relates to the field of health food, in particular to a composition for improving sleep quality and a preparation of the composition. The composition comprises gamma-aminobutric acid and casein hydrolysate. The gamma-aminobutric acid and the casein hydrolysate are composed to use, pentobarbital sodium induced sleeping time can be obviously prolonged, pentobarbital sodium induced sleeping time incubation period is shortened, a function of sleeping improvement is obviously achieved, and good or even better effect can be achieved in case of reducing doses.

The invention belongs to the field of Chinese medicine pharmacy, and relates to an application of a stizolobium cochinchinensis seed extract to preparation of a medicament for treating a Parkinson's disease accompanied by sleep disorder. The stizolobium cochinchinensis seed extract mainly comprises levodopa and stizolobium cochinchinensis seed guanidine components which are purified and combined respectively for preparing a medicament for treating Parkinson's diseases accompanied by sleep disorder of mammals including human beings. As proved by the result of an animal experiment, the stizolobium cochinchinensis seed compound extract can be used for improving the sleeping effect, and has a similar hypnotic effect to a calming effect; the pentobarbital sodium sleeping time can be prolonged, and the anti-shock effect is similar to that of levodopa; and the toxic and side effects are weak. The stizolobium cochinchinensis seed compound extract disclosed by the invention has the advantages of higher bioavailability, shorter response time, longer action maintaining time and the like, can be used for effectively treating Parkinson's diseases accompanied by sleep disorder of mammals including human beings, and has a wide application prospect.

The invention discloses a pharmaceutical composition for treating cardiac failure which is prepared from the following medicinal herbs (by weight ratio): red ginseng 5-12 parts, astragalus root 15-45 parts, black monkshood 5-15 parts, epimedium 8-15 parts, red sage root 10-20 parts, rhizome of Sichuan lovage 5-15 parts, fruit of citron or trifoliate orange 5-15 parts, ophiopogon root 6-15 parts, and lepidium seed 5-15 parts.

The invention discloses applications of tupaia belangeri in preparation of an Alzheimer's disease model. The technical scheme is as follows: S1, an application of the tupaia belangeri, D-galactose, beta-amyloid protein1-42, ibotenic acid and pentobarbital sodium in preparation of the Alzheimer's disease animal model; S2, an application of the tupaia belangeri, D-galactose, beta-amyloid protein1-42, ibotenic acid and pentobarbital sodium in preparation of a tupaia belangeri disease model with at least one of following characteristics: 1), gliocyte proliferation; 2), neurofibril entanglement; 3), learning and memory ability deterioration.

The invention discloses a nattokinase and melatonin composition capable of improving sleep and a preparation method thereof, and belongs to the field of health foods. The composition is prepared fromnattokinase and melatonin, the fibrinolysis activity of the nattokinase in 1g of composition is 500-1000IU, and the content of the melatonin is 0.1mg/g. The preparation method comprises the steps: (1)preparing nattokinase fermentation liquid by using bacillus subtilis capable of producing a high yield of nattokinase, taking the supernatant liquid after centrifuging and performing ultrafiltrationconcentration, and performing vacuum drying to obtain nattokinase freeze-dried powder; and (2) mixing the nattokinase freeze-dried powder with melatonin powder, and adding auxiliary materials. The nattokinase and melatonin composition takes the nattokinase and the melatonin as raw materials, and the two components play a synergistic effect on sleep. Proven by experiments, the nattokinase and melatonin composition disclosed by the invention can obviously prolong the pentobarbital sodium sleep time and shorten the barbital sodium sleep latency, and has a function of enhancing and improving sleep.

The invention relates to the field of traditional Chinese medicines and in particular discloses a traditional Chinese medicine composition for improving sleep. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15-25 parts of spina date seed, 15-25 parts of lucid ganoderma, 15-20 parts of acanthopanax and 5-15 parts of schisandra chinensis. According to the traditional Chinese medicine composition disclosed by the invention, the sleep time of a pentobarbital sodium hypnosis mouse can be prolonged; the sleep onset latency of the pentobarbital sodium hypnosis mouse is shortened; and the traditional Chinese medicine composition does not have an obvious direct sleeping effect and does not have bad effect on the weight of the mouse. The invention also provides a method for preparing the traditional Chinese medicine composition. The traditional Chinese medicine composition disclosed by the invention is a pure traditional Chinese medicine preparation, does not have a toxic or side effect and has good application prospects, and the sleep can be effectively improved.

The invention belongs to the field of medicaments, and in particular relates to a medicinal composition for improving sleep and a preparation method thereof. The invention aims to provide the medicinal composition for improving sleep, which is used for enhancing deep sleep and improving sleep quality. The medicinal composition is prepared by taking the following components in part by weight as raw materials: 6 to 12 parts of lucid ganoderma, 6 to 12 parts of spina date seed, 3 to 8 parts of medlar, 6 to 10 parts of licorice root and 6 to 10 parts of smoked plum; and adding pharmaceutically acceptable auxiliary materials. A pharmacological test proves that the medicinal composition can remarkably prolong sodium pentobarbital sleep time, and has no obvious effect on a latent period of sodium pentobarbital sleep, namely the medicinal composition can be used for enhancing the deep sleep and improving the sleep quality.

The invention provides an adult rat cardiac muscle cell separation method which comprises the steps: (a) preheating a perfusion system and fully cleaning a perfusion pipeline; (b) sequentially injecting heparin sodium and pentobarbital sodium into the abdominal cavity of a rat; (c) quickly opening the chest of the rat to expose the heart, taking the lower heart from the aortic root, putting into aperfusion solution, appropriately cleaning surrounding tissues and ligaturing the aorta on a perfusion needle; (d) connecting the intubated heart with the perfusion system and perfusing the heart bya perfusion solution without Ca<2+>; (e) continuously perfusing the heart by a mixed digestive enzyme solution; (f) taking the perfused heart, putting into a transfer solution, removing the atrium andgreat vessels, shearing the ventricle into small pieces, utilizing a suction pipe to blow and beat tissues into monodispersion cells, utilizing a filter screen to filter cell suspension and standing;(g) restoring Ca<2+> in a gradient mode; (h) precipitating cells after resuspending a complete medium to restore Ca<2+> and inoculating into a coated cell culture bottle to be cultured. By means of the adult rat cardiac muscle cell separation method disclosed by the invention, adult rat cardiac muscle cells which have high quality, high yield and long survival time can be obtained.

The invention discloses a method for producing a cerebral ischemia-reperfusion injury animal model. The method comprises the steps of conducting neck anaesthesia on an experimental animal with pentobarbital sodium, cutting out skin at the two sides of the neck of the experimental animal, separating bilateral arteria carotis communis and pneumogastric nerves, cutting off the bilateral arteria carotis communis or implanting a balloon catheter in the bilateral arteria carotis communis, inflating air into the implanted balloon catheter till the balloon catheter blocks the starting portion of the bilateral arteria carotis communis in the brain, keeping the ischemic state for 2.5-3 hours, and obtaining a cerebral ischemia injury animal; when two hours pass after the bilateral arteria carotis communis is cut off, using a hose for connecting the bilateral arteria carotis communis, conducting suturing, and infusing blood into the hose; or discharging gas out of the balloon catheter, and pouring blood into an ischemic zone once more; conducting neurologic impairment scoring within different time according to requirements, and making records with a video recorder. The method is easy and convenient to operate, the success rate is high, and the reasonable model is provided for cerebral ischemia and reperfusion.

The present invention relates to the technical field of health products, particularly to a sleep improving composition and a preparation method thereof. According to the present invention, apple stem cell freeze-drying powder, melatonin and vitamin B6 are compounded so as to provide characteristics of significant sleep improvement function and no toxic-side effect; experiment results show that the sleep improving effect of the composition of the present invention is significantly superior to the sleep improving effect of the single use of each component, and mouse acute toxicity test results show that the mouse does not present abnormality and no poisoning or death occur; and the falling-asleep occurrence rate of the mice with the sub-threshold dose of sodium pentobarbital can be effectively improved under the composition dose of 0.5 g/kg.

The invention relates to novel application of methylene blue to post-anesthesia awakening. The novel application is characterized in that after an animal is completely anesthetized, a certain dose of the methylene blue can be injected to the animal, so that awakening of the animal can be accelerated, the anesthesia time is shortened, and overdose anesthesia can be reduced to a certain extent; the novel application of the methylene blue is beneficial to relieving and resisting the overdose anesthesia by the aid of the methylene blue in animal experiments and clinical application; the experimental animal is anesthetized by the aid of pentobarbital sodium with certain concentration, then methylene blue solution with certain concentration is injected to the completely anesthetized animal, and the concentration of the methylene blue solution is lower than a toxic dose of the methylene blue; then the awakening time of the animal is observed, and an awakening effect of the methylene blue is judged after the awakening effect of the methylene blue is compared with an awakening effect of normal saline; as shown by experiment results, the required time for awakening the animal after the animal is anesthetized by the aid of the pentobarbital sodium can be obviously shortened by the aid of the methylene blue solution, an awakening latency stage can be shortened, and the overdose anesthesia can be reduced. The novel application of the methylene blue has the advantages that a safe, convenient and speedy awakening measure is hopefully provided for clinical application, and possible injury to patients due to the overdose anesthesia can be reduced.

The invention discloses a method for building a stenosis model after rat carotid artery balloon injuries. The method includes the steps of injecting pentobarbital sodium to the abdominal cavity of a rat to anesthetize the rat, removing furs to prepare the skin, enabling the rat to be fixed on an operation table in a supination mode, cutting the rat along the right middle line of the neck, exposing the left common carotid artery, disinfecting the skin at the position of the groin on the right side of the rat, separating the femoral artery, conducting ligation on the far-heart end at the position 1cm below the branch of the femoral artery, using a needle of a 1m1 injection syringe to puncture the femoral artery, feeding a super-smooth guide wire through the needle of the injection syringe into the carotid artery on the left side, feeding a balloon into the carotid artery on the left side along the super-smooth guide wire, feeding a balloon catheter into the bifurcated position of the internal carotid and external carotid artery of the left side of the neck, enabling the pressure in the balloon to be 1.0 barometric pressure to enable the balloon to right be capable of being pulled in the blood vessel, expanding the balloon to drag the balloon back and forth three times to strip the inner skin to enable the carotid artery to be damaged, withdrawing the balloon catheter, conducting ligation on the near-heart end of a notch of the artery, sewing the skin, and injecting penicillin into the muscle for continuous three days after an operation to avoid infections. According to the method, the operative difficulty in the prior art is reduced, the modeling success rate is improved, and the complication is reduced.

The invention provides a mouse skeletal muscle satellite cell separation and culture method. The mouse skeletal muscle satellite cell separation and culture method comprises following steps: 1, mice are killed through intraperitoneal injection of pentobarbital sodium, and are immersed in 75% alcohol for disinfection; 2, the mice are fixed on plates with the venter posterior surfaces upward, hind limb skin is peeled under aseptic conditions, and thighbone muscle tissues are collected; 3, in an aseptic operation bench, PBS washing is carried out for three times, surface blood vessels, tendons, and connective tissues are removed; 4, the processed tissues are cut into pieces, and are subjected to digestion with preheated trypsin and IV type collagenase respectively; 5, digestion is stopped, centrifugation is carried out, and an obtained supernatant is removed; 6, a cell filtrate is collected, and trypan-blue drying detection is adopted to detect cell activity; 7, after cell counting, a complete medium is adopted for resuspension inoculation into a coated culture flask for culture at 37 DEG C at 5% CO2 environment; 8, differential attachment method is adopted to remove fibrocytes; 9, cell morphology is carried out; 10, ELISA method detection is carried out; and 11, RT-PCR detection is carried out. The mouse skeletal muscle satellite cell separation and culture method is simple in operation, and is high in survival rate; the number of obtained cells is large; the mouse skeletal muscle satellite cell separation and culture method is an ideal method, and is capable of providing experiments with reliable cell resources.

The invention provides a mouse thyroid epithelial cell isolation and culture method. The method includes the steps of: (a) conducting intraperitoneal injection of pentobarbital sodium to kill a mouse, performing alcohol disinfection, than taking out a mouse thyroid tissue connected to a trachea, conducting soaking washing in a precooled sterile double-antibody-containing PBS buffer solution, and stripping the thyroid tissue; (b) carrying out mechanical dissociation of the thyroid tissue, then preheating a mixed enzyme solution and performing shock digestion for 30-40min, using Ficoll to separate thyroid mononuclear cells, and conducting centrifugation to obtain cell precipitate; (c) employing a completely mixed medium to resuspend the cell precipitate, performing inoculation in a treated cell bottle, and carrying out differential adhesion to obtain thyroid epithelial cells; and (d) culturing the purified thyroid epithelial cells in the completely mixed medium. The mouse thyroid epithelial cell isolation and culture method provided by the invention can acquire mouse thyroid epithelial cells with the characteristics of high yield, high purity and long survival time.

The invention discloses a traditional Chinese medicine composition for improving sleep and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials: Codonopsis pilosula, Astragalus root, herba epimedii, semen cuscutae, fructus psoraleae, dogwood, multiflower knotweed root, rhizoma polygonati, oyster, cinnamon, fructus amomi, spina date seed, semen boitae, panaxadiol saponins and other medicinal materials. The invention also provides a preparation method of the traditional Chinese medicine composition for improving sleep. The traditional Chinese medicine composition can prolong the sleeping time of mice dosed by pentobarbital sodium, and can increase the rate of going to sleep, within 30 minutes, of the mice dosage by the pentobarbital sodium, and compared with the prior art, the traditional Chinese medicine composition has an effect on improving the sleep remarkably.

The invention discloses an experimental method for injecting repeating autologous blood clots in dogs. Firstly, healthy crossbred dogs are selected with no regard to gender, wherein the body mass of each dog ranges from 13kg to 19kg, and all the dogs are subjected to a pentobarbital sodium ear edge intravenous injection for anesthesia; after anesthesia, the dogs are fixed on an operating table at a supine position; a trachea cannula is inserted in a trachea and fixed; hair on the neck of each dog is cut, iodophor is applied to the neck for disinfection, the external jugular vein of each dog is exposed, 50mL of blood is collected under an aseptic condition, 200-400U thrombin is added into the blood, and the thrombin and the blood are mixed in a flat vessel and stand for 2 hours at a room temperature to form a thrombus; the thrombus is cut into a cylinder shape with the diameter of 2-4 millimeters as a standby; the external jugular vein of each dog is cut open, a standard 6F three-cavity floating catheter is inserted in the external jugular vein, a catheter is inserted in another incision to the depth of about 15-20 centimeters to reach the right atrium of the dog, and the catheter is connected to a sensor for detection of pulmonary artery pressure. The experimental method for injecting the repeating autologous blood clots in the dogs has the advantages that researches about changes in circulation power in a human body can be more accurate, operation is convenient, and cost is proper.

The invention provides a traditional Chinese medicine compound health food for improving sleep and a preparation method thereof, which relate to the field of a health product. The animal function testproves that two in three experiments of prolongation for sleep time of pentobarbital sodium of mice, the valve dosage hypnotic experiment of pentobarbital sodium, and the barbital sodium sleep latency experiment are positive, no obvious direct sleep effect is generated, the sleep function effect is improved, and no obvious side effect is generated. The traditional Chinese medicine compound healthfood comprises the raw materials by weight percentage: 6-30% of an extract of poria with hostwood, 5-25% of an extract of polygala root, 5-25% of an extract of Chinese magnoliavine, and 6-50% of an extract of spine date seed, wherein the sum of the weight percentages of the raw materials does not exceed 100%.

The invention provides application of aesculi semen or an extract thereof in preparation of sedative-hypnotic drugs. According to the application, the aesculi semen is a commonly-used drug recorded in2015-edition Chinese Pharmacopoeia, the pharmacological experiment results show that the aesculi semen alcohol extract can remarkably prolong the sleeping time of mice caused by a suprathreshold dosage of pentobarbital sodium, the sedative and hypnosis effect is remarkable, and a wide clinical application prospect is achieved.

The invention discloses a biodegradable color printing ink auxiliary which comprises the following main raw materials in parts by weight: 5-10 parts of menthol, 2-6 parts of glaucocalyxin, 17-22 parts of hasmanine, 20-30 parts of 13-dehydroxyindaconintine, 8-17 parts of gomisin G, 15-25 parts of rhoifolin, 11-14 parts of scopolin, 5-9 parts of 2-chlorin-6-fluorophenol, 7-12 parts of chloromethyl methyl ether, 0.5-0.8 part of boron trifluoride methyl alcohol, 2-4 parts of pentobarbital sodium salt, 2-4 parts of 2-aminophenol-4-(2-carboxyl) sulphonanilid and 2-4 parts of cis-dichloride (diethyl sulfide) platinum. The biodegradable color printing ink auxiliary has the advantages of environmental protection, harmless effect, high safety, stable property and good printing effect. When the wastes are recycled, under the effect of a decolorizing agent, the ink can be quickly decolorized, the problem of volatilization of the organic solvent in the oil-based ink can be eliminated and the problems of high deinking difficulty of wastepaper and serious sewage discharge can be solved.

The present invention discloses a method for rapidly and simply preparing a uniform and stable pentobarbital sodium solution. The method comprises: (1) dissolving sodium pentobarbital in ethanol and propylene glycol to prepare a pentobarbital sodium mixture; and (2) using sterile water for injection to carry out volume metering, filtering, and sterilizing to form the sodium pentobarbital solution. According to the present invention, the operation is simple, and the preparation is fast; the concentration of the pentobarbital sodium solution prepared through the method of the present invention can be increased by one time, such that the difficult problem of preparation of the uniform and stable high-concentration pentobarbital sodium solution is solved, and the method can be widely used for preparing the uniform and stable high-concentration pentobarbital sodium solution; and the prepared pentobarbital sodium solution does not produce deterioration and precipitate even after long storage.