48results about How to "Lower index" patented technology

The invention discloses application of a crRNA mediated CRISPR/Cas13a gene editing system in tumor cells. According to the invention, in a U87 glioma cell system, a Cas13a protein can be mediated ontocomplementary target RNA by single-stranded crRNA and is cut. Meanwhile, the Cas13a protein can also trigger a joint shearing effect in eucaryotic cells, i.e., after starting to cut a first piece oftarget RNA, the Cas13a protein carries out aimless random cutting on other encountered RNA so as to take effects of inhibiting and killing tumors, such as effects of reducing a tumor cell index, inhibiting a mouse tumor formation rate and a tumor size. The invention provides a novel method for inhibiting or killing the tumor cells so as to lay a foundation for application of a random shearing effect of the CRISPR-Cas13 system in eukaryotic cells.

The invention provides a method for preparing a 5,6,7,4'-tetrahydroxyflavone compound, which is characterized by comprising the following steps: taking breviscapinun as a raw material, and performing heating reflux reaction between the breviscapinun and alcohol under the catalysis of acid, namely adding 200 to 500 ml of alcohol substance and 1 to 3 ml of 4 to 5 percent acid into 12 to 20 g of the breviscapinun for the heating reflux reaction; and filtering a product, and drying to a constant weight to obtain 5,6,7,4'-tetrahydroxyflavone shown in a formula I. Experiments prove that the 5,6,7,4'-tetrahydroxyflavone shows obvious strengthening effect on acquisition, consolidation and representation of brain memory of a mice model of alzheimer disease, has obvious improving effect on learning memory of the mice model with memory loss caused by anisodine of an M-cholinergic inhibitor, and can obviously improve the recent memory and long-term memory capacities of mice. The experiments also prove that the 5,6,7,4'-tetrahydroxyflavone has the effect of treating arthritis, and also has good treating effects on diabetes mellitus and osteoporosis.

The invention discloses a method for extracting coreopsis tinctoria procyanidins and an application of the coreopsis tinctoria procyanidins in delaying senescence. The method comprises the following steps: extracting coreopsis tinctoria procyanidins from the raw material coreopsis tinctoria which grows 2600m over the sea level by adopting a dynamic high-pressure microfluidization assisted extraction technology for 30 minutes with 70% ethanol at the extracting pressure of 120MPa and the extracting temperature of 50 DEG C, and treating the coreopsis tinctoria twice by means of microjet pressure. The total antioxidation activity of the prepared procyanidins is higher than that of ascorbic acid, and has an effect of delaying senescence of drosophila melanogaster by combining results of drosophila melanogaster surviving experiments and in-vivo biochemical index detection; liver injury experiments in mice indicate that the procyanidins extracted from coreopsis tinctoria is capable of reducing the activities of ALT (alanine aminotransferase) and AST (aspartate aminotransferase) in serum and playing a certain role in protecting CC14 caused liver injury in mice. According to the application, the procyanidins extracted from coreopsis tinctoria have an excellent effect of delaying senescence, and has a wide practical value.

The invention discloses an anti-inebriation and anti-alcohol homologous traditional Chinese medicine composition and a preparation method of the traditional Chinese medicine composition, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from homologous raw traditional Chinese medicines such as semen hoveniae, radix puerariae, tangerine peel and liquorice root, wherein the mass ratio of the semen hoveniae to the radix puerariae to the tangerine peel to the liquorice root is (7.5-60):(7.5-60):(3-15):(3-12). The preparation method comprises the following steps: weighing the raw material medicines according to the mass ratio, adding water which is 8-12 times of the total mass of the raw material medicines, performing soaking treatment for 1-3h and then reflux extraction for 1-2h, filtering, collecting a filtrate, centrifugally collecting supernatant, concentrating after filtering, and drying after freeze-drying, thereby obtaining the anti-inebriation and anti-alcohol homologous traditional Chinese medicine composition extract. In the extraction and preparation process, no any chemical reagents and toxic substances are used, the selected medicine is wide, the preparation operation is simple and easy, safety and effectiveness are achieved, the treatment purpose is definite, and the traditional Chinese medicine composition has a good health food and medicine development potential.

The invention discloses a traditional Chinese medicine composition for treating non-alcoholic fatty liver disease. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: corydalis saxicola, rhizoma picrorhizae, pseudo-ginseng, radix salviae miltiorrhizae, kudzuvine root, rhizoma atractylodis macrocephalae, radix paeoniae alba, tuckahoe, radix bupleuri, angelica, lotus leaves, semen coicis, chinese waxgourd semen, reed rhizome, platycodon grandiflorus, semen lepidii, loquat leaves, fritillaria cirrhosa, fructus lycii, semen cassia, rhizoma alismatis, peach kernel and mulberry leaves. Pharmacological experiments prove that the traditional Chinese medicine composition is capable of preventing insulin resistance, improving insulin sensitivity, reducing blood fat, inhibiting inflammatory reaction, improving abnormal glucolipid metabolism, reducing hepatocyte fat deposition, improving liver function, decreasing liver indexes and having obvious treatment effects on a rat suffering from non-alcoholic fatty liver disease.

The invention relates to baking soda and biological compound enzyme toothpaste. An effective composition comprises food-grade baking soda and an enzyme preparation, wherein the enzyme preparation comprises muramidase and beta-glucanase. The toothpaste is prepared from a friction agent, a humectant, a surfactant, a thickening agent, an acid-alkali buffering agent, the food-grade baking soda, the enzyme preparation, a taste improvement agent, a stain-removing and whitening agent, essence and water according to a certain weight ratio. According to the baking soda and biological compound enzyme toothpaste, the enzyme deeply enters tooth gaps by utilizing properties of weak basicity, diffusivity, permeability, strong cleaning power and the like of baking soda particles, and each part of an oralcavity is cleaned to help to regulate the acid alkali balance of the oral cavity; meanwhile, the activity of a biological compound enzyme which is used as a main antibacterial component can be enhanced and an unique bacterium-dissolving and antibacterial mechanism is formed; harmful bacteria of the oral cavity is effectively inhibited, the attachment of the bacteria is reduced, the dental plaqueindex is reduced and the health of the oral cavity is commonly maintained.

The invention discloses a synthetic peptide vaccine of B cell epitope based on TGF(transforming growth factor)-beta1 and application thereof, inhibits auxo-action of the cell factor TGF-B1-BB during the development process of liver fibrosis so as to inhibit the development of liver fibrosis to a some extent by constructing two epitope peptides of AY25 and LS30, coupling carrier proteins to synthesize an TGF-beta1 peptide vaccine, immunizing an ICR mice by intraperitoneal injection to induce the same to generate an anti-TGF-beta1 antibody, and neutralizing the cell factor TGF-B1-BB over expressed during CCL4 induced hepatic fibrosis process.

The invention discloses a preparation of an additive for reducing the liver fat content of a turbot and the application of the additive in reducing the liver fat content of a turbot, wherein the preparation of the additive is implemented through the following steps: through taking minced pacific Pollock as raw materials, hashing the pacific Pollock, then raising the temperature of the obtained pacific Pollock for sterilization; after the temperature of the sterilized pacific Pollock raises to 90 DEG C, carrying out heat preservation on the pacific Pollock, then carrying out sterilization on the pacific Pollock for 20 minutes; adding water and a protease preparation into the obtained pacific Pollock and then mixing; carrying out enzymolysis on the obtained mixture under the condition of optimized enzymolysis process parameters; raising the temperature of the obtained enzymatic hydrolysate so as to carry out inactivation on proteases in the enzymatic hydrolysate; carrying out rough filtration on the enzymatic hydrolysate so as to separate spurs from the enzymatic hydrolysate; carrying out centrifugation on the obtained enzymatic hydrolysate so as to respectively obtain soluble hydrolytic fish protein powder; and carrying out ultrafiltration-purifying on the obtained soluble hydrolytic fish protein powder, then freezing and drying the obtained soluble hydrolytic fish protein powder so as to obtain hydrolytic fish protein powder, wherein the protease preparation refers to Alcalase + Flavourzyme; the enzymolysis process parameters are as follows: enzymolysis temperature is 50-60 DEG C; starting pH value is 8.5, ratio of material to water is 1:1, and enzymolysis time is 3 hours. Through the preparation disclosed by the invention, the hydrolytic fish protein powder (the molecular weight thereof is less than 100 Da and reaches 30%) can be obtained. When the hydrolytic fish protein powder is used for replacing 5% of fish protein concentrate in a turbot feed.

The invention discloses a medicinal application of epimedin C in treatment on diabetic liver injury, and belongs to the technical field of biological medicines. The invention discloses that epimedin Ccan improve the cholesterol level of high-density lipoprotein in the liver of a diabetic model animal for the first time, the cholesterol level of low-density lipoprotein in the liver is reduced, theproblem of lipid metabolism disorder is relieved, a liver index is reduced, the activity of superoxide dismutase and glutathione peroxidase in the liver is enhanced, the content of malondialdehyde isreduced, the oxidative stress state of the liver is improved, normal forms and structures of hepatocytes and tissues are maintained, the activity levels of glutamic-pyruvic transaminase and glutamicoxalacetic transaminase in the liver are reduced, and liver function repair is promoted, so that the epimedin C can be used for preparing medicines for treating diabetic liver injury. The epimedin C is used for preparing the medicine for treating diabetic liver injury in the form of a medicinal preparation, and comprises buccal tablets, coated tablets and hard capsules. The invention provides a basis for treatment on diabetic liver injury, and also provides an idea for deep development and utilization of epimedium resources.

The invention discloses a medicinal composition for treating hyperplasia of prostate and belongs to the field of Chinese traditional medicine modernization. Active components of the medicinal composition for treating hyperplasia of prostate are prepared from the following raw materials in parts by weight: 10-25 parts of red sage root, 6-15 parts of radix paeoniae rubrathe, 6-15 parts of Japanese eupatorium, 6-15 parts of combined spicebush, 6-15 parts of psoralea corylifolia, 6-15 parts of prepared rehmannia root, 8-18 parts of polyporus, 8-18 parts of dianthus superbus, and 10-25 parts of prunella vulgaris. The medicinal composition has remarkable treatment effect, small drug dose, quick effectiveness, few side effects and good stability. The medicinal composition is simple in preparation technology and low in cost, and is suitable for large-scale production.

The invention provides a lactic acid bacteria composite microecological preparation for improving development of glandular stomach of poultry and an application of the lactic acid bacteria composite microecological preparation. The lactobacillus composite microecological preparation comprises limosilactobacillus reuteri powder with the viable bacterium content not lower than 2.00 * 10 <8> CFU/g, bifidobacterium animalis powder with the viable bacterium content not lower than 5.00 * 10 <7> CFU/g, lactobacillus paracasei powder with the viable bacterium content not lower than 5.00 * 10 <7> CFU/g and a carrier with the viable bacterium content not lower than 500 mg/g. The lactic acid bacteria composite microecological preparation can remarkably reduce the glandular stomach index of broiler chickens, reduce the glandular stomach and muscle stomach ratio, improve the glandular stomach nipple and mucosa integrity and effectively improve the glandular stomach development of the broiler chickens, has the advantages of safety, no residues, no side effects and the like, and has wide application value.

The invention discloses an outer wall of a building and a method for preventing PM2.5 through the outer wall. The outer wall comprises a plurality of wall units. Each wall unit comprises two shape steel columns on the two sides, a shape steel column in the middle and a concrete wall body (1) connecting the three shape steel columns, wherein a main ventilation hole (2) is formed in the concrete wall (1) in a penetrating mode, filtering holes (3) communicated with the main ventilation hole (2) are formed in webs of the three shape steel columns, the concrete wall body (1) is provided with secondary ventilation holes (5) in a penetrating mode in the thickness direction, and the secondary ventilation holes (5) are communicated with the main ventilation hole (2). An outer door (6) is arranged on the outer sides of every two adjacent shape steel columns, every two adjacent shape steel columns, one outer door (6) and one concrete wall body (1) define an outer cavity (8), and a filtering screen (9) is arranged in each outer cavity (8). The method is characterized in that inner doors (7) are closed, the outer doors (6) are opened, an air pump is arranged at the end of a main air flue (4), and the air pump is driven to suck air. By means of the outer wall and the method, the PM2.5 index can be effectively reduced.

The invention discloses a lotus leaf composition and application thereof. The lotus leaf composition is prepared by mixing lotus leaf powder, mulberry leaf powder and sweet potato leaf powder according to the mass ratio of (8-1):(1-8):(1-8), has the effects of regulating blood lipid and blood glucose and preventing and treating the fatty liver and can be used for preparing food, healthcare food and medicine for regulating blood lipid and preventing, relieving and treating the non-alcoholic fatty liver disease. The lotus leaf composition is from medicinal and edible traditional Chinese medicinal materials and food, is suitable for being eaten for a long time, and will bring good social benefits.

The invention provides fructus gardeniae oral liquid capable of protecting the liver and benefiting the gallbladder. The oral liquid is prepared from, by weight, 10-50 parts of fructus gardeniae, 10-20 parts of herba artemisiae capillariae, 10-20 parts of poria cocos, 10-20 parts of herba lysimachiae, 10-20 parts of rhizoma cyperi, 10-20 parts of radix paeoniae rubra, 10-20 parts of herba leonuri, 10-20 parts of corn stigma, 10-20 parts of radix trichosanthis, 10-20 parts of rehmannia roots, 10-20 parts of rhizoma polygoni cuspidati, 10-20 parts of rhizoma anemarrhenae, 10-20 parts of herba patriniae, 10-20 parts of fructus forsythiae, 10-20 parts of rhizoma sparganii, 10-20 parts of radix scutellariae, 10-20 parts of rhizoma polygonati, 10-20 parts of curcuma zedoary, 10-20 parts of herba ecliptae and 10-20 parts of herba plantaginis. Fructus grdeniae can clear heat, discharge fire and cool the blood. The oral liquid can treat pyreticosis of dysphoria and insomnia, jaundice, gonorrhea, consumptive thirst, hot eyes, pharyngalgia, hematemesis, hemorrhinia, bloody flux, hematuria, heat-toxicity sore and ulcer and sprain swelling.

The invention puts forward application of solanum lycopersicum stem and leaf extracts for treating alcohol induced liver injury, and relates to the technical field of medicines. An alcohol induced liver injury mouse is experimented and researched to discover for the first time that the solanum lycopersicum stem and leaf extracts effectively alleviate mouse hyperlipemia caused by alcohol excessivedrinking, lower a liver index, recover a liver function, alleviate liver inflammation and lipidosis, have an excellent treatment effect for liver injuries, and provide an effective scientific basis for developing the solanum lycopersicum stem and leaf extracts into a medicine capable of preventing and treating the alcoholic liver disease.

The invention relates to a chewing gum containing coix seeds and a preparation method thereof. The chewing gum is prepared from coix seed extract, poria cocos, herba menthae, chrysanthemum flower extract, a gum base, a sweetener and essence. The preparation method comprises the following steps: crushing all the components; uniformly mixing; pouring and molding; cooling. All traditional Chinese medicine components in the chewing gum provided by the invention complement each other and commonly have the effects of clearing damp and promoting diuresis, strengthening spleen and harmonizing stomach, inhibiting bacteria and diminishing inflammation; the chewing gum is used for clinically treating gingivitis; active pharmaceutical ingredients can directly act on dental plaques and are directly absorbed through oral mucosa; the dental plaque index and the gingivitis index are remarkably reduced, and the chewing gum has a remarkable oral cavity health-care effect.

The invention discloses application of short-chain fatty acid in prevention or/and treatment of liver injury. The short-chain fatty acid is a product of intestinal flora glycolysis dietary fibers, belongs to an endogenous substance, is small in toxic and side effects, simple in structure, easy to metabolize, convenient to obtain and low in price, and can inhibit liver fatty degeneration and liver inflammation, improve liver functions and prevent or/and treat liver injury.