Preparation and application of additive for reducing liver fat content of turbot

Abstract

The invention discloses a preparation of an additive for reducing the liver fat content of a turbot and the application of the additive in reducing the liver fat content of a turbot, wherein the preparation of the additive is implemented through the following steps: through taking minced pacific Pollock as raw materials, hashing the pacific Pollock, then raising the temperature of the obtained pacific Pollock for sterilization; after the temperature of the sterilized pacific Pollock raises to 90 DEG C, carrying out heat preservation on the pacific Pollock, then carrying out sterilization on the pacific Pollock for 20 minutes; adding water and a protease preparation into the obtained pacific Pollock and then mixing; carrying out enzymolysis on the obtained mixture under the condition of optimized enzymolysis process parameters; raising the temperature of the obtained enzymatic hydrolysate so as to carry out inactivation on proteases in the enzymatic hydrolysate; carrying out rough filtration on the enzymatic hydrolysate so as to separate spurs from the enzymatic hydrolysate; carrying out centrifugation on the obtained enzymatic hydrolysate so as to respectively obtain soluble hydrolytic fish protein powder; and carrying out ultrafiltration-purifying on the obtained soluble hydrolytic fish protein powder, then freezing and drying the obtained soluble hydrolytic fish protein powder so as to obtain hydrolytic fish protein powder, wherein the protease preparation refers to Alcalase + Flavourzyme; the enzymolysis process parameters are as follows: enzymolysis temperature is 50-60 DEG C; starting pH value is 8.5, ratio of material to water is 1:1, and enzymolysis time is 3 hours. Through the preparation disclosed by the invention, the hydrolytic fish protein powder (the molecular weight thereof is less than 100 Da and reaches 30%) can be obtained. When the hydrolytic fish protein powder is used for replacing 5% of fish protein concentrate in a turbot feed.

Description

Technical field: [0001] The invention belongs to the compounding technology of turbot compound feed, and relates to the preparation and application of an additive for reducing turbot fatty liver. Background technique: [0002] Fatty Liver Syndrome has been reported in pigs, chickens, cattle, sheep, dogs, fish, mink and other animals. A large number of animal fatty liver research data show that genetics, nutrition, management, environment, hormones, toxic substances and other reasons can lead to the occurrence of fatty liver disease. The pathogenesis is related to fat metabolism, which is generally believed to be caused by increased fat synthesis and decreased oxidation in hepatocytes, and nutritional balance plays a very important role in this process. [0003] The formation of fish fatty liver is mainly caused by the imbalance of nutrients it needs and the lack of some anti-fatty liver factors. At the same time, it is also affected by the physiological and metabolic chara...

AI Technical Summary

Problems solved by technology

Supplementing these substances in the feed is beneficial to the absorption and utilization of lipids by fish, improvi

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