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Method for extracting coreopsis tinctoria procyanidins and application of coreopsis tinctoria procyanidins in delaying senescence

A technology of proanthocyanidins and extraction method, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., to achieve good antioxidant activity

Inactive Publication Date: 2014-12-03
新疆泸疆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, scholars have studied the application of DHPM in extraction and macromolecular modification, such as the extraction and activity of total flavonoids in potato leaves, the influence of papain activity, the preparation of fatty acid liposomes, the activity of trypsin, and the stability of DHPM. However, there is no relevant research report on the application of DHPM technology to the extraction of snow chrysanthemum proanthocyanidins

Method used

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  • Method for extracting coreopsis tinctoria procyanidins and application of coreopsis tinctoria procyanidins in delaying senescence
  • Method for extracting coreopsis tinctoria procyanidins and application of coreopsis tinctoria procyanidins in delaying senescence
  • Method for extracting coreopsis tinctoria procyanidins and application of coreopsis tinctoria procyanidins in delaying senescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Extraction of Xinjiang Kunlun snow chrysanthemum proanthocyanidins

[0048] The present invention specifically provides a method for extracting Kunlun snow chrysanthemum proanthocyanidins, % is calculated by weight percentage, and the specific extraction method steps are as follows:

[0049] (1) Kunlun snow chrysanthemum grown at an altitude of more than 2,600 meters is used as the raw material. The dried snow chrysanthemum powder is crushed in a crusher and passed through a 40-mesh sieve.

[0050] (2) Degrease snow inulin with petroleum ether for 8 hours and dry at 50°C for 2 hours.

[0051] (3) Ethanol infiltration: The 70% ethanol extract prepared with a solid-to-liquid ratio of 1:40 by weight was placed in a refrigerator at 4°C overnight.

[0052] (4) Pre-homogenization: use a homogenization pressure of 30MPa, and homogenize twice.

[0053] (5) Dynamic ultra-high pressure micro-jet treatment: use a certain micro-jet pressure to process twice; extracti...

Embodiment 2

[0057] Example 2: Determination of the Optimal Process for Extracting Xinjiang Kunlun Snow Chrysanthemum Proanthocyanidins

[0058] Ethanol with a concentration of 60% (V / V) was selected as the extraction solvent, and the leaching was repeated twice. A single factor experiment was performed with four factors: ethanol concentration, solid-liquid ratio, extraction time and extraction temperature. The acquisition rate and DPPH·clearance rate are indicators for evaluating the extraction process conditions.

[0059] 1.1 Effect of ethanol concentration on extraction yield and DPPH clearance rate

[0060] Accurately weigh 1.000g snow chrysanthemum pollen, degrease with petroleum ether until colorless and dry, the ratio of solid to liquid is 1:40, and the concentration of 40%, 50%, 60%, 70%, 80%, 90% ethanol is placed at 4°C Stand in the refrigerator overnight, after 30 MPa, homogenize twice, treat twice under the micro jet pressure of 100 MPa, extract in 50°C water bath for 30 min...

Embodiment 3

[0072] Example 3: Determination of the Optimal Technology of Xinjiang Kunlun Snow Chrysanthemum Proanthocyanidins

[0073] The four-factor three-level L9 (34) orthogonal optimization experiment was designed with ethanol concentration, extraction temperature, extraction time, and microjet pressure as independent variables for the extraction process of proanthocyanidins from Kunlun snow chrysanthemum.

[0074] serial number A pressure (Mpa) BExtraction temperature / ℃ C ethanol concentration D Extraction time Extraction rate / % 1 1（80） 1（30） 1（50） 1（20） 16.84 2 1 2（40） 2（60） 2（30） 17.00 3 1 3（50） 3（70） 3（40） 19.80 4 2（100） 1 2 3 17.70 5 2 2 3 1 20.40 6 2 3 1 2 21.90 7 3（120） 1 3 2 19.30 8 3 2 1 3 20.30 9 3 3 2 1 20.70 K1 17.880 17.947 19.680 19.313 K2 20.000 19.233 18.467 19.400 K3 20.100 20.800 19.833 19.267 R 2.220 2.853 1.366 0.133 ...

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Abstract

The invention discloses a method for extracting coreopsis tinctoria procyanidins and an application of the coreopsis tinctoria procyanidins in delaying senescence. The method comprises the following steps: extracting coreopsis tinctoria procyanidins from the raw material coreopsis tinctoria which grows 2600m over the sea level by adopting a dynamic high-pressure microfluidization assisted extraction technology for 30 minutes with 70% ethanol at the extracting pressure of 120MPa and the extracting temperature of 50 DEG C, and treating the coreopsis tinctoria twice by means of microjet pressure. The total antioxidation activity of the prepared procyanidins is higher than that of ascorbic acid, and has an effect of delaying senescence of drosophila melanogaster by combining results of drosophila melanogaster surviving experiments and in-vivo biochemical index detection; liver injury experiments in mice indicate that the procyanidins extracted from coreopsis tinctoria is capable of reducing the activities of ALT (alanine aminotransferase) and AST (aspartate aminotransferase) in serum and playing a certain role in protecting CC14 caused liver injury in mice. According to the application, the procyanidins extracted from coreopsis tinctoria have an excellent effect of delaying senescence, and has a wide practical value.

Description

technical field [0001] The invention relates to the technical field of extraction and preparation of proanthocyanidins. Specifically, the present invention relates to a method for extracting proanthocyanidins from Kunlun snow chrysanthemum and the technical field of its application. Background technique [0002] Xinjiang Kunlun snow chrysanthemum, the scientific name is two-color coreopsis ( Coreopsis tinctoria ) is the dry flower head of an annual herb of the genus Coreopsis in the Asteraceae family. In Xinjiang, it is mainly distributed in the Kunlun Mountains with an altitude of about 3,000 meters in the Hotan area, where there are relatively rich wild resources. For a long time, Kunlun snow chrysanthemum has been used as scented tea by local residents, and it has also been used as a Uighur medicinal material in Xinjiang Uyghur Hospital. It has the functions of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, harmonizing the stomach...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P39/06C07D311/62A61K31/352
Inventor 敬思群柴文杰
Owner 新疆泸疆生物科技有限公司
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