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Method for preparing taro tuber ethanol extract and anti-oxidation application of taro tuber ethanol extract

An ethanol extraction and soybean taro technology, applied in the field of medicine, can solve the problem that the antioxidant activity of soybean taro tuber extract is rarely reported, and achieve the effects of protecting liver cells from oxidative damage, expanding application fields, and having good development prospects.

Active Publication Date: 2018-03-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, at present, many nutrition and health food researchers are very concerned about the research and topic of anti-oxidation and the occurrence and prevention of chronic diseases, but there are few reports on the antioxidant activity of bean and taro tuber extracts

Method used

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  • Method for preparing taro tuber ethanol extract and anti-oxidation application of taro tuber ethanol extract
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  • Method for preparing taro tuber ethanol extract and anti-oxidation application of taro tuber ethanol extract

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Embodiment 1

[0052] Embodiment 1, a kind of preparation method of bean taro tuber ethanol extract, carries out following steps successively:

[0053] 1), in the ratio of 1g:5ml, mix the bean and taro tubers with 95% ethanol containing 0.1mol / L hydrochloric acid, and use a juice extractor to beat; After extracting for 240 ± 10 minutes, the extract was centrifuged (4000 rpm for 30 minutes), and the centrifuged filter residue replaced the potato tubers in the above steps to repeat the beating, ultrasonic extraction and centrifugation of the above steps; the number of repetitions was 3 times;

[0054] The supernatants obtained by the above 3 times of centrifugation were combined to carry out the following step 2).

[0055] 2), the obtained supernatant was concentrated by rotary evaporation in a rotary evaporator (the process parameters set by the rotary evaporation were temperature 38°C, vacuum degree -0.09MPa) until it was 10% of the original volume;

[0056] 3), the concentrated solution ob...

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Abstract

The invention discloses a method for preparing a taro tuber ethanol extract. The method comprises the following steps: mixing taro tubers with 95% ethanol with 0.1 mol / L of hydrochloric acid, pulping,performing ultrasonic extraction, and centrifuging to obtain supernate; concentrating the supernate, and performing separation purification on the obtained concentrated liquid with macroporous resin;using a 1% formic acid solution as an eluting agent and a methanol solution with 0.2% of formic acid as an eluent, and collecting an elution liquid; concentrating the elution liquid, pre-freezing thepulp concentrated liquid at minus 70 DEG C to minus 90 DEG C firstly, and further drying by a vacuum freeze-drying machine, thereby obtaining powder, namely the taro tuber ethanol extract. The invention further discloses application of the taro tuber ethanol extract to preparation of liver cell oxidation damage inhibitors.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method of ethanol extract of bean and taro tubers and its anti-oxidation application. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitamin E and minerals. Among the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P39/06A61P1/16A23L33/105A61K125/00
CPCA23V2002/00A61K36/48A61K2236/333A61K2236/51A61K2236/55A23L33/105A23V2250/21A23V2200/30
Inventor 郑晓冬楚强俞露霜李永璐刘阳阳
Owner ZHEJIANG UNIV
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