Application of solanum lycopersicum stem and leaf extracts for treating alcohol induced liver injury

An extract and liver damage technology, applied in the field of medicine, can solve problems such as liver tissue damage, achieve the effect of restoring liver function, relieving liver inflammation and excellent therapeutic effect

Inactive Publication Date: 2020-09-29
赵晓娟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drugs such as corticosteroids, antioxidants, colchicine, pentoxifylline, and lipid-lowering drugs have certain effects, but not all cases of alcoholic liver disease are curative, or only for a certain period of disease, a certain Aspects such as reducing the mortality rate, reducing the incidence of liver cirrhosis, etc. have a certain effect, even under the action of a single drug, it will cause irreversible damage to the liver tissue

Method used

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  • Application of solanum lycopersicum stem and leaf extracts for treating alcohol induced liver injury
  • Application of solanum lycopersicum stem and leaf extracts for treating alcohol induced liver injury
  • Application of solanum lycopersicum stem and leaf extracts for treating alcohol induced liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Tomato stem and leaf extract and its application in the treatment of alcoholic liver injury.

[0034] Preparation method of tomato stem and leaf extract (hot water extraction method):

[0035] 1) Hot water extraction:

[0036] The tomato stems and leaves are dried at a constant temperature of 50°C, pulverized by a pulverizer, and passed through a 100-mesh sieve. Take tomato stem and leaf powder and add distilled water at a ratio of 1:20 to liquid, and soak at room temperature for 4 hours. Electric heating mantle boiling water bath for 1h, filter through 8 layers of gauze, collect the filtrate, and extract the filter residue twice. The three extracts were combined, centrifuged at 4000r / min for 15min, the supernatant was collected, marked and stored at 4°C.

[0037] 2) Extract concentration:

[0038] Turn on the condensing device of the rotary evaporator, turn on the circulating water, and turn off the deflation button. Transfer an appropriate volume of tomato stem a...

Embodiment 2

[0041] Animal and drug handling methods:

[0042] Select 50 Kunming male mice (about 20g-22g), divide them into five groups at random, and mark them as blank control group, model group, low-concentration group, high-concentration group and drug control group after 7 days of adaptive feeding. All mice were free to ingest feed and water during the experiment, and the doses of gavage and intraperitoneal injection were both 0.1 mL / 10 g body weight. specific:

[0043] Blank control group: intragastric administration of normal saline in the morning and intraperitoneal injection of 0.1 mL / 10 g body weight of normal saline in the afternoon, with an interval of not less than 4 hours.

[0044] Model group: intragastric administration of 40% alcohol in the morning and intraperitoneal injection of normal saline in the afternoon.

[0045] Low-concentration group: intragastric administration of 40% alcohol in the morning, and intraperitoneal injection of 0.05g / mL tomato stem and leaf extrac...

Embodiment 3

[0053] Biochemical index detection: based on the serum and liver tissue collected in Example 2, the calculation of mouse liver index (such as figure 1 ), the determination of mouse serum total antioxidant capacity (T-AOC) (such as figure 2 ), determination of mouse serum AST and ALT (such as image 3 ), serum total cholesterol (TC) level, serum triglyceride (TG) level, serum low-density lipoprotein (LDL-c) level (such as Figure 4 ).

[0054] In this application, the results are expressed as Mean±S.E.M. One-Way ANOVA combined with Dunnett'sposthoc test was used to analyze the differences between groups. Statistically significant difference was indicated by P<0.05. Graphs were drawn using GraphPad Prism 5 software (GraphPad Software, Inc., San Diego, CA).

[0055] Wherein, liver index=(liver wet weight / last mouse body weight)×100%;

[0056] Total antioxidant capacity (T-AOC) of mouse serum was determined according to the instructions of the T-AOC kit, and the level of se...

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Abstract

The invention puts forward application of solanum lycopersicum stem and leaf extracts for treating alcohol induced liver injury, and relates to the technical field of medicines. An alcohol induced liver injury mouse is experimented and researched to discover for the first time that the solanum lycopersicum stem and leaf extracts effectively alleviate mouse hyperlipemia caused by alcohol excessivedrinking, lower a liver index, recover a liver function, alleviate liver inflammation and lipidosis, have an excellent treatment effect for liver injuries, and provide an effective scientific basis for developing the solanum lycopersicum stem and leaf extracts into a medicine capable of preventing and treating the alcoholic liver disease.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of tomato stem and leaf extracts in the treatment of alcohol-induced liver damage. Background technique [0002] Alcoholic liver disease (ALD) is a chronic liver disease caused by long-term drinking, and its lesion degree is closely related to the amount of drinking, which seriously endangers public health. From the 1970s to the present, the proportion of drinking population and the prevalence of ALD in my country have shown an upward trend. [0003] ALD is not a static pathological state and there is a risk of continued progression. Studies have shown that after ethanol enters the liver cells, it is oxidized to acetaldehyde through two pathways: liver alcohol dehydrogenase, hydrogen peroxide decomposing enzyme and liver mitochondrial alcohol oxidase. Acetaldehyde has obvious toxic effects on liver cells and can directly or indirectly lead to liver cell necrosi...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P1/16A61P3/06
CPCA61K36/81A61P1/16A61P3/06
Inventor 赵晓娟王丽郭林林马婧铭朱彤彤贾广青吴雯雯
Owner 赵晓娟
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