Application of solanum lycopersicum stem and leaf extracts for treating alcohol induced liver injury
An extract and liver damage technology, applied in the field of medicine, can solve problems such as liver tissue damage, achieve the effect of restoring liver function, relieving liver inflammation and excellent therapeutic effect
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Embodiment 1
[0033] Tomato stem and leaf extract and its application in the treatment of alcoholic liver injury.
[0034] Preparation method of tomato stem and leaf extract (hot water extraction method):
[0035] 1) Hot water extraction:
[0036] The tomato stems and leaves are dried at a constant temperature of 50°C, pulverized by a pulverizer, and passed through a 100-mesh sieve. Take tomato stem and leaf powder and add distilled water at a ratio of 1:20 to liquid, and soak at room temperature for 4 hours. Electric heating mantle boiling water bath for 1h, filter through 8 layers of gauze, collect the filtrate, and extract the filter residue twice. The three extracts were combined, centrifuged at 4000r / min for 15min, the supernatant was collected, marked and stored at 4°C.
[0037] 2) Extract concentration:
[0038] Turn on the condensing device of the rotary evaporator, turn on the circulating water, and turn off the deflation button. Transfer an appropriate volume of tomato stem a...
Embodiment 2
[0041] Animal and drug handling methods:
[0042] Select 50 Kunming male mice (about 20g-22g), divide them into five groups at random, and mark them as blank control group, model group, low-concentration group, high-concentration group and drug control group after 7 days of adaptive feeding. All mice were free to ingest feed and water during the experiment, and the doses of gavage and intraperitoneal injection were both 0.1 mL / 10 g body weight. specific:
[0043] Blank control group: intragastric administration of normal saline in the morning and intraperitoneal injection of 0.1 mL / 10 g body weight of normal saline in the afternoon, with an interval of not less than 4 hours.
[0044] Model group: intragastric administration of 40% alcohol in the morning and intraperitoneal injection of normal saline in the afternoon.
[0045] Low-concentration group: intragastric administration of 40% alcohol in the morning, and intraperitoneal injection of 0.05g / mL tomato stem and leaf extrac...
Embodiment 3
[0053] Biochemical index detection: based on the serum and liver tissue collected in Example 2, the calculation of mouse liver index (such as figure 1 ), the determination of mouse serum total antioxidant capacity (T-AOC) (such as figure 2 ), determination of mouse serum AST and ALT (such as image 3 ), serum total cholesterol (TC) level, serum triglyceride (TG) level, serum low-density lipoprotein (LDL-c) level (such as Figure 4 ).
[0054] In this application, the results are expressed as Mean±S.E.M. One-Way ANOVA combined with Dunnett'sposthoc test was used to analyze the differences between groups. Statistically significant difference was indicated by P<0.05. Graphs were drawn using GraphPad Prism 5 software (GraphPad Software, Inc., San Diego, CA).
[0055] Wherein, liver index=(liver wet weight / last mouse body weight)×100%;
[0056] Total antioxidant capacity (T-AOC) of mouse serum was determined according to the instructions of the T-AOC kit, and the level of se...
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