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Application of crRNA-mediated CRISPR/Cas13a gene editing system in tumor cells

A gene editing and tumor cell technology, applied in the application field of CRISPR/Cas13a gene editing system in tumor cells, can solve the problem of less application of CRISPR/Cas13a system, reduce tumor cell index, inhibit tumor formation rate, and inhibit tumor effect of size

Active Publication Date: 2021-07-09
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Claims
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Problems solved by technology

[0003] In order to solve the problem that the CRISPR / Cas13a system is rarely used in eukaryotic and tumor cells, the present invention proposes the application of a crRNA-mediated CRISPR / Cas13a gene editing system in tumor cells

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  • Application of crRNA-mediated CRISPR/Cas13a gene editing system in tumor cells
  • Application of crRNA-mediated CRISPR/Cas13a gene editing system in tumor cells
  • Application of crRNA-mediated CRISPR/Cas13a gene editing system in tumor cells

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0024]Cas13a基因序列为:ATGAAAGTGACCAAGGTCGACGGCATCAGCCACAAGAAGTACATCGAAGAGGGCAAGCTCGTGAAGTCCACCAGCGAGGAAAACCGGACCAGCGAGAGACTGAGCGAGCTGCTGAGCATCCGGCTGGACATCTACATCAAGAACCCCGACAACGCCTCCGAGGAAGAGAACCGGATCAGAAGAGAGAACCTGAAGAAGTTCTTTAGCAACAAGGTGCTGCACCTGAAGGACAGCGTGCTGTATCTGAAGAACCGGAAAGAAAAGAACGCCGTGCAGGACAAGAACTATAGCGAAGAGGACATCAGCGAGTACGACCTGAAAAACAAGAACAGCTTCTCCGTGCTGAAGAAGATCCTGCTGAACGAGGACGTGAACTCTGAGGAACTGGAAATCTTTCGGAAGGACGTGGAAGCCAAGCTGAACAAGATCAACAGCCTGAAGTACAGCTTCGAAGAGAACAAGGCCAACTACCAGAAGATCAACGAGAACAACGTGGAAAAAGTGGGCGGCAAGAGCAAGCGGAACATCATCTACGACTACTACAGAGAGAGCGCCAAGCGCAACGACTACATCAACAACGTGCAGGAAGCCTTCGACAAGCTGTATAAGAAAGAGGATATCGAGAAACTGTTTTTCCTGATCGAGAACAGCAAGAAGCACGAGAAGTACAAGATCCGCGAGTACTATCACAAGATCATCGGCCGGAAGAACGACAAAGAGAACTTCGCCAAGATTATCTACGAAGAGATCCAGAACGTGAACAACATCAAAGAGCTGATTGAGAAGATCCCCGACATGTCTGAGCTGAAGAAAAGCCAGGTGTTCTACAAGTACTACCTGGACAAAGAGG...

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Abstract

The invention discloses the application of a crRNA-mediated CRISPR / Cas13a gene editing system in tumor cells. In the U87 glioma cell line, the Cas13a protein of the present invention can be mediated by a single-stranded crRNA to a complementary target RNA and cut. At the same time, Cas13a protein can also trigger the effect of joint shearing in eukaryotic cells, that is, after starting to cut the first entry of RNA, Cas13a protein will randomly cut other RNAs encountered without purpose, thereby reducing Tumor cell index, inhibition of tumor formation rate and tumor size in mice and other effects of inhibiting and killing tumors. The invention provides a new method for inhibiting or killing tumor cells, and lays the foundation for the application of the random shearing effect of the CRISPR-Cas13 system in eukaryotic cells.

Description

technical field [0001] The present invention relates to DNA recombination technology, more specifically the application of a crRNA-mediated CRISPR / Cas13a gene editing system in tumor cells. Background technique [0002] Short palindromic repeats (Clustered regularly interspaced shot palindromicrepeat, CRISPR) and CRISPR-associated proteins (Cas) are adaptive immune systems of archaea and bacteria. Cas proteins are mainly divided into two categories: adaptation module and effector module. The acquisition module can import exogenous nucleic acid information into the CRISPR sequence and generate CRISPR RNAs (crRNAs) as a guide for the system. Under the mediation of crRNA, the effector module cleaves the nucleic acid invaded by exogenous sources. In the CRISPR-Cas system, the acquired modules have a high degree of similarity, mainly including Cas1 and Cas2. In contrast, there are many types of effector systems, which are mainly divided into Class 1 systems (C1) and Class 2 sy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/90C12N15/113
CPCC12N15/113C12N15/907C12N2310/10C12N2310/20
Inventor 康春生王琦雪周俊虎
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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