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Lactic acid bacteria composite microecological preparation for improving development of glandular stomach of poultry and application of lactic acid bacteria composite microecological preparation

A technology of compound microecology and lactic acid bacteria, applied in the direction of application, microorganism, lactobacillus, etc., can solve the problems of no synergistic effect of compound strains, weak effect of lactic acid bacteria, and reduced use effect, so as to prevent diseases such as glandular gastritis and repair Gastrointestinal mucosal tissue, the effect of improving integrity

Pending Publication Date: 2021-11-09
山东益昊生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many types of lactic acid bacteria, and the effect of a single lactic acid bacteria is not strong, and some biological agents prepared by using lactic acid bacteria have defects such as high prices, too complex or single strains, resulting in low viable bacteria, or the combination of strains. There is no synergistic effect, and even antagonism will affect its effect, thereby reducing the use effect and increasing the use cost

Method used

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  • Lactic acid bacteria composite microecological preparation for improving development of glandular stomach of poultry and application of lactic acid bacteria composite microecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, screening and identification of bacterial strains

[0029] 1. Screening and identification of Lactobacillus reuteri NSL0501

[0030] 1. The traditional fermented milk fans from herdsmen in Ganzi County, Ganzi Tibetan Autonomous Prefecture, Sichuan Province were used as samples. Transfer the sample to a sterilized mortar for grinding, add sterile water to dilute to 10 ml of sample solution, and then use sterile water again according to 10 -3 -10 -4 After dilution, 200 μl were respectively applied to the modified MRS fixed medium, and cultured at 37 ℃ for 16 hours. Pick a single colony on the plate, inoculate the single colony in the improved MRS liquid medium, and culture it statically at 36 °C for 16 h, and repeatedly streak the cultured bacterial solution on the improved MRS solid plate by the streaking method. Purification was performed and the purification was repeated 3 times. After three generations of purification, the obtained single colony numb...

Embodiment 2

[0048] Embodiment 2, the preparation of lactic acid bacteria compound probiotics

[0049] The lactic acid bacteria compound microecological preparation of the present invention includes: the content of live bacteria is not less than 2.00×10 8 CFU / g of Lactobacillus reuteri NSL0501, the live bacteria content is not less than 5.00×10 7 CFU / g Bifidobacterium animalis NSY0201, the live bacteria content is not less than 5.00×10 7 CFU / g of Lactobacillus paracasei NSL0201, the carrier maltodextrin content is not less than 500 mg / g.

[0050] The above three strains were streaked on the improved MRS solid plate, cultured at 37°C for 48 hours, then picked a single colony and inoculated in the improved MRS liquid medium, and cultured at 37°C for 16 hours; the bacterial liquid was then inoculated with 3% inoculum Into 2 L of modified MRS liquid medium, cultured at 37°C for 16 h, and cultured to the logarithmic phase; the bacterial suspension was centrifuged at 12000 r / min for 10 min ...

Embodiment 3

[0052] Embodiment 3, the application of lactic acid bacteria compound microecological preparation in broiler breeding

[0053] The lactic acid bacteria compound microecological preparation is added to the full-price compound feed at a mass of 2‰-5‰ of the total weight of the full-price compound feed.

[0054] 400 1-day-old Ross 308 broilers who were healthy and had no significant difference in body weight were randomly divided into two treatment groups, fed with full-price compound pellet feed; among them, group I was the control group, and no microecological additives were added to the feed; group II As the experimental group, 3‰ of lactic acid bacteria compound microecological preparation was added to the feed (3kg of lactic acid bacteria compound microecological preparation was added per ton of full-price feed); each treatment group had 5 repetitions, and each repetition group had 40 chickens.

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Abstract

The invention provides a lactic acid bacteria composite microecological preparation for improving development of glandular stomach of poultry and an application of the lactic acid bacteria composite microecological preparation. The lactobacillus composite microecological preparation comprises limosilactobacillus reuteri powder with the viable bacterium content not lower than 2.00 * 10 <8> CFU / g, bifidobacterium animalis powder with the viable bacterium content not lower than 5.00 * 10 <7> CFU / g, lactobacillus paracasei powder with the viable bacterium content not lower than 5.00 * 10 <7> CFU / g and a carrier with the viable bacterium content not lower than 500 mg / g. The lactic acid bacteria composite microecological preparation can remarkably reduce the glandular stomach index of broiler chickens, reduce the glandular stomach and muscle stomach ratio, improve the glandular stomach nipple and mucosa integrity and effectively improve the glandular stomach development of the broiler chickens, has the advantages of safety, no residues, no side effects and the like, and has wide application value.

Description

technical field [0001] The invention mainly relates to the technical field of microbes and feed additives, in particular to a lactic acid bacteria compound microecological preparation for improving poultry glandular stomach development and application thereof. Background technique [0002] As an important digestive organ of chicken, glandular stomach plays an important role in the digestion of chicken diet. There are two types of glands in the glandular gastric mucosa: the superficial layer is a single tubular gland that secretes mucus; the deep layer is a multiple tubular gland that mainly secretes hydrochloric acid and pepsin. Damage to the glandular stomach may lead to hypersecretion of hydrochloric acid, leading to excessive secretion of hydrochloric acid, which will affect the pH of the glandular stomach and gizzard, and then affect digestion, and cause corrosion to the glandular gastric mucosa and gizzard corneum, resulting in glandular gastric mucosa and gizzard The ...

Claims

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Application Information

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IPC IPC(8): A23K50/75A23K10/18A23K20/163C12N1/20C12R1/225C12R1/01
CPCA23K50/75A23K10/18A23K20/163C12N1/20A23V2400/165A23V2400/173A23V2400/515
Inventor 王晓伟赵孟孟荆学金荆忠平张明俊赵志强高素芹武传菊
Owner 山东益昊生物科技有限公司
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