Lactic acid bacteria composite microecological preparation for improving development of glandular stomach of poultry and application of lactic acid bacteria composite microecological preparation

A technology of compound microecology and lactic acid bacteria, applied in the direction of application, microorganism, lactobacillus, etc., can solve the problems of no synergistic effect of compound strains, weak effect of lactic acid bacteria, and reduced use effect, so as to prevent diseases such as glandular gastritis and repair Gastrointestinal mucosal tissue, the effect of improving integrity

Pending Publication Date: 2021-11-09
山东益昊生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many types of lactic acid bacteria, and the effect of a single lactic acid bacteria is not strong, and some biological agents prepared by using lactic acid bacteria have defects such as high prices, too com

Method used

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  • Lactic acid bacteria composite microecological preparation for improving development of glandular stomach of poultry and application of lactic acid bacteria composite microecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1, screening and identification of strains

[0029] I. Screening identification of Roysh Lactobacillus NSL0501

[0030] 1. Since its since the Sichuan Ganzi Tibetan Autonomous Prefecture, Ganzi County, the traditional fermentation fumes of the herdsmen. The sample was transferred into a sterilized mortar, and the sterile water was added to 10 mL of sample liquid, and then use sterile water again 10 -3 -10 -4 After the ratio was diluted, 200 μl was taken to modified MRS fixed medium, and the culture was allowed to stand at 37 ° C for 16 h. Pick the single colonies on the plate and inoculate the single colon into the modified MRS liquid medium, and then placed at 36 ° C for 16 h, and the cultured bacterial solution is repeated on the modified MRS solid plate by scribing method. Purification was purified and purified 3 times. After purifying the three generations, the resulting single colony number was NSL0501.

[0031] Strain NSL0501 in the modified MRS fixed plate of...

Example Embodiment

[0048] Example 2, preparation of lactic acid bacteria composite microorganism formulations

[0049] The lactic acid composite microorganism formulation according to the present invention includes: the source of live bacteria is not less than 2.00 × 10 8 CFU / G of Roysh Lactobacillus NSL0501, live bacteria content is not less than 5.00 × 10 7 CFU / G of animal bifidobacteria NSY0201, live bacteria content is not less than 5.00 × 10 7 CFU / G of the sub-cheese NSL0201, the carrier maltodextrin content is not less than 500 mg / g.

[0050] The above three strains were discharged from the modified MRS solid plate overline, 37 ° C for 48 h, and then picked up a single bacterius into the modified MRS liquid medium, 37 ° C culture 16 h; the bacterial solution was inoculated at 3% inoculation. In the modified MRS liquid medium of 2 L, 37 ° C was cultured for 16 h, cultured to the alignment; the bacterial suspension was at 4 ° C, 12000 r / min, centrifugal 10 min, abandon the supernatant,...

Example Embodiment

[0052] Example 3. Application of lactic acid bacteria composite microorganism formulation in broiler breeding

[0053] The lactic acid bacteria composite microorganism formulation is added to the full price of 2 ‰ -5 ‰ of the total weight of the total price.

[0054] Rose 308 broilers who were 6 days of 1 day-old and non-weight difference were randomly divided into two treatment groups, feeding full price combined with particulate feed; wherein i group is a control group, and no microecological additives are added in the feed; Group II group For the experimental group, 3 ‰ of lactic acid bacteria composite microorganism formulations were added to the feed (3kg lactic acid bacteria complex microecological preparations per ton); 5 repetitions were set in each treatment group, 40 chickens per repeat group.

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Abstract

The invention provides a lactic acid bacteria composite microecological preparation for improving development of glandular stomach of poultry and an application of the lactic acid bacteria composite microecological preparation. The lactobacillus composite microecological preparation comprises limosilactobacillus reuteri powder with the viable bacterium content not lower than 2.00 * 10 <8> CFU/g, bifidobacterium animalis powder with the viable bacterium content not lower than 5.00 * 10 <7> CFU/g, lactobacillus paracasei powder with the viable bacterium content not lower than 5.00 * 10 <7> CFU/g and a carrier with the viable bacterium content not lower than 500 mg/g. The lactic acid bacteria composite microecological preparation can remarkably reduce the glandular stomach index of broiler chickens, reduce the glandular stomach and muscle stomach ratio, improve the glandular stomach nipple and mucosa integrity and effectively improve the glandular stomach development of the broiler chickens, has the advantages of safety, no residues, no side effects and the like, and has wide application value.

Description

technical field [0001] The invention mainly relates to the technical field of microbes and feed additives, in particular to a lactic acid bacteria compound microecological preparation for improving poultry glandular stomach development and application thereof. Background technique [0002] As an important digestive organ of chicken, glandular stomach plays an important role in the digestion of chicken diet. There are two types of glands in the glandular gastric mucosa: the superficial layer is a single tubular gland that secretes mucus; the deep layer is a multiple tubular gland that mainly secretes hydrochloric acid and pepsin. Damage to the glandular stomach may lead to hypersecretion of hydrochloric acid, leading to excessive secretion of hydrochloric acid, which will affect the pH of the glandular stomach and gizzard, and then affect digestion, and cause corrosion to the glandular gastric mucosa and gizzard corneum, resulting in glandular gastric mucosa and gizzard The ...

Claims

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Application Information

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IPC IPC(8): A23K50/75A23K10/18A23K20/163C12N1/20C12R1/225C12R1/01
CPCA23K50/75A23K10/18A23K20/163C12N1/20A23V2400/165A23V2400/173A23V2400/515
Inventor 王晓伟赵孟孟荆学金荆忠平张明俊赵志强高素芹武传菊
Owner 山东益昊生物科技有限公司
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