Lactic acid bacteria composite microecological preparation for improving development of glandular stomach of poultry and application of lactic acid bacteria composite microecological preparation
A technology of compound microecology and lactic acid bacteria, applied in the direction of application, microorganism, lactobacillus, etc., can solve the problems of no synergistic effect of compound strains, weak effect of lactic acid bacteria, and reduced use effect, so as to prevent diseases such as glandular gastritis and repair Gastrointestinal mucosal tissue, the effect of improving integrity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Embodiment 1, screening and identification of bacterial strains
[0029] 1. Screening and identification of Lactobacillus reuteri NSL0501
[0030] 1. The traditional fermented milk fans from herdsmen in Ganzi County, Ganzi Tibetan Autonomous Prefecture, Sichuan Province were used as samples. Transfer the sample to a sterilized mortar for grinding, add sterile water to dilute to 10 ml of sample solution, and then use sterile water again according to 10 -3 -10 -4 After dilution, 200 μl were respectively applied to the modified MRS fixed medium, and cultured at 37 ℃ for 16 hours. Pick a single colony on the plate, inoculate the single colony in the improved MRS liquid medium, and culture it statically at 36 °C for 16 h, and repeatedly streak the cultured bacterial solution on the improved MRS solid plate by the streaking method. Purification was performed and the purification was repeated 3 times. After three generations of purification, the obtained single colony numb...
Embodiment 2
[0048] Embodiment 2, the preparation of lactic acid bacteria compound probiotics
[0049] The lactic acid bacteria compound microecological preparation of the present invention includes: the content of live bacteria is not less than 2.00×10 8 CFU / g of Lactobacillus reuteri NSL0501, the live bacteria content is not less than 5.00×10 7 CFU / g Bifidobacterium animalis NSY0201, the live bacteria content is not less than 5.00×10 7 CFU / g of Lactobacillus paracasei NSL0201, the carrier maltodextrin content is not less than 500 mg / g.
[0050] The above three strains were streaked on the improved MRS solid plate, cultured at 37°C for 48 hours, then picked a single colony and inoculated in the improved MRS liquid medium, and cultured at 37°C for 16 hours; the bacterial liquid was then inoculated with 3% inoculum Into 2 L of modified MRS liquid medium, cultured at 37°C for 16 h, and cultured to the logarithmic phase; the bacterial suspension was centrifuged at 12000 r / min for 10 min ...
Embodiment 3
[0052] Embodiment 3, the application of lactic acid bacteria compound microecological preparation in broiler breeding
[0053] The lactic acid bacteria compound microecological preparation is added to the full-price compound feed at a mass of 2‰-5‰ of the total weight of the full-price compound feed.
[0054] 400 1-day-old Ross 308 broilers who were healthy and had no significant difference in body weight were randomly divided into two treatment groups, fed with full-price compound pellet feed; among them, group I was the control group, and no microecological additives were added to the feed; group II As the experimental group, 3‰ of lactic acid bacteria compound microecological preparation was added to the feed (3kg of lactic acid bacteria compound microecological preparation was added per ton of full-price feed); each treatment group had 5 repetitions, and each repetition group had 40 chickens.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com