A traditional Chinese medicine preparation for treating diabetic nephropathy, and preparation method and application thereof

Abstract

The present invention discloses a traditional Chinese medicine preparation for treating diabetic nephropathy, which is prepared from rhubarb polysaccharides, the total flavone of scutellaria root, total alkaloids of rhizoma coptis, and pharmaceutically acceptable auxiliary materials. Pharmacodynamic trials proved that the present invention has the effects of decreasing the blood glucose of the normal mouse, reducing the area under blood glucose concentration curve, and improving the sugar tolerance of the mouse; the present invention can improve the multi-food, the will drink, and the polyuriasymptoms of diabetic nephropathy model rats, reduce urinary protein and urinary albumin, decrease glycosylated hemoglobin, serum cholesterol and serum triglyceride, decrease overhigh creatinine clearance rate, reducing the renal index, alleviate the renal lesion, and have the effect for antagonizing early diabetic nephropathy. In addition, the present invention also discloses a preparation methodof the traditional Chinese medicine preparation and application in preparing a medicament for preventing and controlling diabetic nephropathy.

Description

technical field

[0001] The invention relates to a traditional Chinese medicine preparation for preventing and treating diabetic nephropathy; the invention also relates to a preparation method of the traditional Chinese medicine preparation and its application in preparing medicine for preventing and treating diabetic nephropathy. Background technique

[0002] According to the report of the World Health Organization (WHO), there are as many as 125 million diabetic (diabetic mellitus, hereinafter referred to as DM) patients worldwide, and it is expected to increase to 380 million by 2025. In recent years, the incidence rate in my country has increased at an alarming rate. With the rapid development of the national economy and the further improvement of people's living standards, the incidence rate will continue to increase. It is estimated that the total number of diabetes patients today is more than 30 million. It is expected to exceed 50 million by 2010, becoming the country...

Examples

Embodiment 1

[0025] 1.1 Preparation of rhubarb polysaccharide:

[0026] Weigh 400g of rhubarb medicinal material, grind it into a coarse powder, first use 95% ethanol to reflux and extract 3 times, add 5 times the amount of 95% ethanol each time, reflux for 1 hour, discard the filtrate; evaporate the dregs to dry the solvent, add distilled water to decoct and extract 3 times, add 10 times the amount of water each time and decoct for 2 hours, combine the filtrate, concentrate to the crude extract; cool the crude extract to room temperature, add 95% ethanol to make the alcohol content reach 80%, let stand for 12 hours, filter The precipitate was obtained; the precipitate was fully dissolved with distilled water, centrifuged at 5,000 rpm for 15 minutes, and the supernatant was taken; ultrafiltration was performed to obtain an ultrafiltrate with a molecular weight above 10,000, concentrated, and dried at low temperature to obtain 5.3 g of rhubarb polysaccharide.

[0027] Determination of polys...

Embodiment 2

[0047] 2.1 Preparation of rhubarb polysaccharide:

[0048] Weigh 8000g of rhubarb medicinal material, crush it into a coarse powder, reflux and extract with 95% ethanol for 3 times, add 5 times the amount of 95% ethanol each time, reflux for 1 hour, discard the filtrate; Each time, add 10 times the amount of water to decoct for 2 hours, combine the filtrate, concentrate to the crude extract; cool the crude extract to room temperature, add 95% ethanol to make the alcohol content reach 80%, let stand for 12 hours, filter The precipitate was obtained; the precipitate was fully dissolved with distilled water, centrifuged at 5000 rpm for 15 minutes, and the supernatant was taken; ultrafiltration was performed to obtain an ultrafiltrate with a molecular weight above 10,000, concentrated, and dried at low temperature to obtain 106.6 g of rhubarb polysaccharide.

[0049] Determination of polysaccharide content by phenol-sulfuric acid method: Weigh 45.00mg of rhubarb polysaccharide, ad...

technical field

[0001] The invention relates to a traditional Chinese medicine preparation for preventing and treating diabetic nephropathy; the invention also relates to a preparation method of the traditional Chinese medicine preparation and its application in preparing medicine for preventing and treating diabetic nephropathy. Background technique

[0002] According to the report of the World Health Organization (WHO), there are as many as 125 million diabetic (diabetic mellitus, hereinafter referred to as DM) patients worldwide, and it is expected to increase to 380 million by 2025. In recent years, the incidence rate in my country has increased at an alarming rate. With the rapid development of the national economy and the further improvement of people's living standards, the incidence rate will continue to increase. It is estimated that the total number of diabetes patients today is more than 30 million. It is expected to exceed 50 million by 2010, becoming the country...

Examples

Embodiment 1

[0025] 1.1 Preparation of rhubarb polysaccharide:

[0026] Weigh 400g of rhubarb medicinal material, grind it into a coarse powder, first use 95% ethanol to reflux and extract 3 times, add 5 times the amount of 95% ethanol each time, reflux for 1 hour, discard the filtrate; evaporate the dregs to dry the solvent, add distilled water to decoct and extract 3 times, add 10 times the amount of water each time and decoct for 2 hours, combine the filtrate, concentrate to the crude extract; cool the crude extract to room temperature, add 95% ethanol to make the alcohol content reach 80%, let stand for 12 hours, filter The precipitate was obtained; the precipitate was fully dissolved with distilled water, centrifuged at 5,000 rpm for 15 minutes, and the supernatant was taken; ultrafiltration was performed to obtain an ultrafiltrate with a molecular weight above 10,000, concentrated, and dried at low temperature to obtain 5.3 g of rhubarb polysaccharide.

[0027] Determination of polys...

Embodiment 2

[0047] 2.1 Preparation of rhubarb polysaccharide:

[0048] Weigh 8000g of rhubarb medicinal material, crush it into a coarse powder, reflux and extract with 95% ethanol for 3 times, add 5 times the amount of 95% ethanol each time, reflux for 1 hour, discard the filtrate; Each time, add 10 times the amount of water to decoct for 2 hours, combine the filtrate, concentrate to the crude extract; cool the crude extract to room temperature, add 95% ethanol to make the alcohol content reach 80%, let stand for 12 hours, filter The precipitate was obtained; the precipitate was fully dissolved with distilled water, centrifuged at 5000 rpm for 15 minutes, and the supernatant was taken; ultrafiltration was performed to obtain an ultrafiltrate with a molecular weight above 10,000, concentrated, and dried at low temperature to obtain 106.6 g of rhubarb polysaccharide.

[0049] Determination of polysaccharide content by phenol-sulfuric acid method: Weigh 45.00mg of rhubarb polysaccharide, ad...

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