A traditional Chinese medicine preparation for treating diabetic nephropathy, and preparation method and application thereof
A technology for diabetic nephropathy and traditional Chinese medicine preparation, applied in the application field of medicine, can solve the problems of nephrotoxicity and the like
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Embodiment 1
[0025] 1.1 Preparation of rhubarb polysaccharide:
[0026] Weigh 400g of rhubarb medicinal material, grind it into a coarse powder, first use 95% ethanol to reflux and extract 3 times, add 5 times the amount of 95% ethanol each time, reflux for 1 hour, discard the filtrate; evaporate the dregs to dry the solvent, add distilled water to decoct and extract 3 times, add 10 times the amount of water each time and decoct for 2 hours, combine the filtrate, concentrate to the crude extract; cool the crude extract to room temperature, add 95% ethanol to make the alcohol content reach 80%, let stand for 12 hours, filter The precipitate was obtained; the precipitate was fully dissolved with distilled water, centrifuged at 5,000 rpm for 15 minutes, and the supernatant was taken; ultrafiltration was performed to obtain an ultrafiltrate with a molecular weight above 10,000, concentrated, and dried at low temperature to obtain 5.3 g of rhubarb polysaccharide.
[0027] Determination of polys...
Embodiment 2
[0047] 2.1 Preparation of rhubarb polysaccharide:
[0048] Weigh 8000g of rhubarb medicinal material, crush it into a coarse powder, reflux and extract with 95% ethanol for 3 times, add 5 times the amount of 95% ethanol each time, reflux for 1 hour, discard the filtrate; Each time, add 10 times the amount of water to decoct for 2 hours, combine the filtrate, concentrate to the crude extract; cool the crude extract to room temperature, add 95% ethanol to make the alcohol content reach 80%, let stand for 12 hours, filter The precipitate was obtained; the precipitate was fully dissolved with distilled water, centrifuged at 5000 rpm for 15 minutes, and the supernatant was taken; ultrafiltration was performed to obtain an ultrafiltrate with a molecular weight above 10,000, concentrated, and dried at low temperature to obtain 106.6 g of rhubarb polysaccharide.
[0049] Determination of polysaccharide content by phenol-sulfuric acid method: Weigh 45.00mg of rhubarb polysaccharide, ad...
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