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Application of crRNA mediated CRISPR/Cas13a gene editing system in tumor cells

A tumor cell and gene editing technology, applied in the field of DNA recombination, can solve the problem of less application of CRISPR/Cas13a system, and achieve the effect of reducing tumor cell index, inhibiting tumor formation rate and tumor size

Active Publication Date: 2018-09-18
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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Problems solved by technology

[0003] In order to solve the problem that the CRISPR / Cas13a system is rarely used in eukaryotic and tumor cells, the present invention proposes the application of a crRNA-mediated CRISPR / Cas13a gene editing system in tumor cells

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  • Application of crRNA mediated CRISPR/Cas13a gene editing system in tumor cells
  • Application of crRNA mediated CRISPR/Cas13a gene editing system in tumor cells
  • Application of crRNA mediated CRISPR/Cas13a gene editing system in tumor cells

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0024]Cas13a基因序列为:ATGAAAGTGACCAAGGTCGACGGCATCAGCCACAAGAAGTACATCGAAGAGGGCAAGCTCGTGAAGTCCACCAGCGAGGAAAACCGGACCAGCGAGAGACTGAGCGAGCTGCTGAGCATCCGGCTGGACATCTACATCAAGAACCCCGACAACGCCTCCGAGGAAGAGAACCGGATCAGAAGAGAGAACCTGAAGAAGTTCTTTAGCAACAAGGTGCTGCACCTGAAGGACAGCGTGCTGTATCTGAAGAACCGGAAAGAAAAGAACGCCGTGCAGGACAAGAACTATAGCGAAGAGGACATCAGCGAGTACGACCTGAAAAACAAGAACAGCTTCTCCGTGCTGAAGAAGATCCTGCTGAACGAGGACGTGAACTCTGAGGAACTGGAAATCTTTCGGAAGGACGTGGAAGCCAAGCTGAACAAGATCAACAGCCTGAAGTACAGCTTCGAAGAGAACAAGGCCAACTACCAGAAGATCAACGAGAACAACGTGGAAAAAGTGGGCGGCAAGAGCAAGCGGAACATCATCTACGACTACTACAGAGAGAGCGCCAAGCGCAACGACTACATCAACAACGTGCAGGAAGCCTTCGACAAGCTGTATAAGAAAGAGGATATCGAGAAACTGTTTTTCCTGATCGAGAACAGCAAGAAGCACGAGAAGTACAAGATCCGCGAGTACTATCACAAGATCATCGGCCGGAAGAACGACAAAGAGAACTTCGCCAAGATTATCTACGAAGAGATCCAGAACGTGAACAACATCAAAGAGCTGATTGAGAAGATCCCCGACATGTCTGAGCTGAAGAAAAGCCAGGTGTTCTACAAGTACTACCTGGACAAAGAGG...

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Abstract

The invention discloses application of a crRNA mediated CRISPR / Cas13a gene editing system in tumor cells. According to the invention, in a U87 glioma cell system, a Cas13a protein can be mediated ontocomplementary target RNA by single-stranded crRNA and is cut. Meanwhile, the Cas13a protein can also trigger a joint shearing effect in eucaryotic cells, i.e., after starting to cut a first piece oftarget RNA, the Cas13a protein carries out aimless random cutting on other encountered RNA so as to take effects of inhibiting and killing tumors, such as effects of reducing a tumor cell index, inhibiting a mouse tumor formation rate and a tumor size. The invention provides a novel method for inhibiting or killing the tumor cells so as to lay a foundation for application of a random shearing effect of the CRISPR-Cas13 system in eukaryotic cells.

Description

technical field [0001] The present invention relates to DNA recombination technology, more specifically the application of a crRNA-mediated CRISPR / Cas13a gene editing system in tumor cells. Background technique [0002] Short palindromic repeats (Clustered regularly interspaced shot palindromicrepeat, CRISPR) and CRISPR-associated proteins (Cas) are adaptive immune systems of archaea and bacteria. Cas proteins are mainly divided into two categories: adaptation module and effector module. The acquisition module can import exogenous nucleic acid information into the CRISPR sequence and generate CRISPR RNAs (crRNAs) as a guide for the system. Under the mediation of crRNA, the effector module cleaves the nucleic acid invaded by exogenous sources. In the CRISPR-Cas system, the acquired modules have a high degree of similarity, mainly including Cas1 and Cas2. In contrast, there are many types of effector systems, which are mainly divided into Class 1 systems (C1) and Class 2 sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/90C12N15/113
CPCC12N15/113C12N15/907C12N2310/10C12N2310/20
Inventor 康春生王琦雪周俊虎
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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