Adult rat cardiac muscle cell separation method
A cardiomyocyte and separation method technology, applied in cell dissociation methods, animal cells, vertebrate cells, etc., can solve the problems of difficulty in culturing adult rat cardiomyocytes, and achieve the effects of high yield, long survival time and high activity
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[0022] In order to present the purpose and advantages of the present invention more clearly, specific embodiments will now be further described. The specific embodiments described here are only for explaining the present invention, and are not intended to limit the present invention.
[0023] The experimental equipment used in this experiment is as follows:
[0024] Inverted microscope (XDS-1A, Shanghai), ultra-clean bench (model: SW-CJ-1FD, Suzhou ultra-clean); cell culture box (model: 240i Thermo), super constant temperature water bath (model: HH-501 Shanghai Chuangyi Science and Education Equipment Co., Ltd.), constant flow pump (Longer Pump, BT 100-1L), langendorff perfusion system (Beijing Zhongshi Dichuang Technology Development Co., Ltd.).
[0025] In the present invention, SD rats with a body weight of 150-300 g are selected to be fed for 6-8 weeks, and cardiomyocytes of adult rats are isolated. The specific operation is as follows:
[0026] 1. Preheat the perfusion...
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