Placenta preserving fluid

A technology for preserving liquid and placenta, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problem of decreased bacterial infection rate of the placenta, and achieve the effect of avoiding the decrease of cell activity.

Active Publication Date: 2014-04-09
章毅 +10
View PDF11 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

AIM-V medium contains L-glutamine, and after adding antibiotics, the infection rate of placenta can be reduced, but the number of stem cells obtained is not significantly different from that of normal saline

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Placenta preserving fluid
  • Placenta preserving fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of embodiment 1 placenta preservation solution

[0039] With deionized water as solvent, add 10mmol-15mmol potassium dihydrogen phosphate, 30mmol-40mmol sodium chloride, 40mmol-50mmol potassium chloride, 8mmol-10mmol magnesium sulfate, 70mmol-100mmol histidine, 1.5mmol-2.0mmol allopurine , 45mg-55mg5,7-dihydroxy-3',4'-dimethylflavone, 3mmol reduced glutathione, 1ml-8ml2-mercaptoethanol, 8mol-10mmol adenosine, 20mg-50mg ginsenoside Rg1, 0.01 Mmol-0.08mmol dexamethasone, 100000U-1000000U penicillin, 60mg-100mg streptomycin and 50mg-70mg gentamicin were prepared into 1000ml placental preservation solution, pH7.33-7.83. Subpackage, seal, and store at 0°C-4°C.

Embodiment 2

[0040] The proliferative ability of embodiment 2 cells

[0041] The placenta was preserved in normal saline, and after 5 days, the placenta was taken out and mesenchymal stem cells were isolated (see figure 1 ).

[0042] The placenta was placed in the placenta preservation solution prepared in Example 1, and after 5 days, the placenta was taken out and separated to obtain mesenchymal stem cells (see figure 2 ).

[0043] from figure 1 with figure 2 It can be seen that the morphology of stem cells is better maintained after using the placenta preservation solution. By calculating the number of cells, it is proved that the placental cells proliferating ability is stronger after the placenta preservation solution is preserved, and the number of mesenchymal stem cells cultured at the same time is more. The results are shown in Table 1.

[0044] Table 1

[0045]

Embodiment 3

[0046] The infection rate of embodiment 3 placenta

[0047] The placenta was stored in physiological saline and the placenta preservation solution prepared in Example 1 for 5 days, and then the bacterial infection test was carried out (see Table 2).

[0048] Table 2

[0049]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to placenta preserving fluid with pH value of 7.33-7.83; the placenta preserving fluid per 1,000ml contains 10-15mmol of potassium dihydrogen phosphate, 30-40mmol of sodium chloride, 40-50mmol of potassium chloride, 8-10mmol of magnesium sulfate, 70-100mmol of histidine, 1.5-2.0mmol of allopurinol, 3mmol of reduced glutathione, 1-8ml of 2-mercaptoethanol, 8-10mmol of adenosine, 1,000,00-1,000,000U of penicillin, 60-100mg of streptomycin and 50-70mg of gentamicin. The placenta preserving fluid can store the delivered placenta for a long time, prevent from reduction of cell activity and control the bacteria contamination rate of the placenta to be in a low level.

Description

technical field [0001] The invention relates to a composition for tissue preservation, in particular to a placenta preservation solution for in vitro preservation of placenta. Background technique [0002] The human placenta is composed of amniotic membrane, chorion and decidua basalis. The amniotic membrane and chorion are of fetal origin, and the decidua of different regions are of maternal origin. In addition to its important role in fetal development, nutritional support and maintenance of tolerance, the placenta is also a reservoir of stem cells. Placental tissue is easy to obtain, easy to handle, does not require invasive operations, and its research will not involve ethical and moral issues, so it has become a research hotspot to find new sources of human stem cells. [0003] The discovery of placental stem cells solved the source of stem cells. There are almost no restrictions on the placenta, and the placenta can be obtained on the basis of the informed consent o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 章毅霍思维陆瑶洪艳
Owner 章毅
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap