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Lactobacillus brevis capable of relieving anxiety and improving sleep and application of lactobacillus brevis

A technology of Lactobacillus brevis and strains, applied in the field of microbiology, can solve problems such as inability to sleep and improve sleep, and achieve the effect of prolonging sleep time, improving sleep, and excellently simulating the survival of the gastrointestinal tract

Active Publication Date: 2019-08-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the above patents do not involve lactic acid bacteria that have excellent gastrointestinal tract survival ability, cell adhesion ability and GABA production ability at the same time, and cannot really be used to improve sleep

Method used

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  • Lactobacillus brevis capable of relieving anxiety and improving sleep and application of lactobacillus brevis
  • Lactobacillus brevis capable of relieving anxiety and improving sleep and application of lactobacillus brevis
  • Lactobacillus brevis capable of relieving anxiety and improving sleep and application of lactobacillus brevis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Screening of Lactobacillus brevis GDMCC No.60605

[0056] 1 Experimental method

[0057] Strains were screened using fresh human feces and pickled vegetable water as samples.

[0058] (1) Pipette 0.5 mL of the sample into 5 mL of MRS medium, culture at 37° C. for 18 to 24 hours for enrichment.

[0059] (2) Gradient dilution: absorb 0.5mL of the enriched sample into 4.5mL sterile saline to obtain 10 -1 Diluent, then draw 0.5mL 10 -1 Diluted solution in 4.5mL normal saline to obtain 10 -2 Diluent, according to this operation, successively get 10 -3 , 10 -4 , 10 -5 , 10 -6 Diluent.

[0060] (3) Plating culture: draw 100 μL of gradient dilution and spread it on the MRS plate, 10 -4 , 10 -5 , 10 -6 One plate per gradient; cultured at 37°C for 48h.

[0061] (4) Separation by streaking: Select colonies with typical characteristics on the coated plate according to the shape, size, edge, and transparency of the colony, pick the colonies with an inoculation ...

Embodiment 2

[0072] Example 2 Study on the ability of Lactobacillus brevis GDMCC No.60605 to produce GABA

[0073] 1 Test method

[0074] 1.1 Activation of Lactobacillus brevis GDMCC No.60605

[0075] Lactobacillus brevis GDMCC No.60605 was inoculated into MRS medium at an inoculation amount of 2%, and cultured at 37°C for 18 hours to obtain activated Lactobacillus brevis GDMCC No.60605;

[0076] 1.2 Preparation of fermentation broth

[0077] Take 2% of the bacterial solution activated twice in 1.1 and inoculate it into the MMRS medium supplemented with 1.5% L-sodium glutamate (L-MSG) and incubate at 37° C. for 48 hours to obtain the fermentation solution to be tested.

[0078] The formula of MMRS medium is (g / L): on the basis of MRS medium, 1.5% sodium L-glutamate is added as a precursor substance for GABA synthesis.

[0079] 1.3 Determination of GABA in fermentation broth

[0080] The GABA content in the fermentation supernatant was determined using HPLC. The analysis conditions wer...

Embodiment 3

[0085] Example 3 Exploration of the Survival Ability of Lactobacillus brevis GDMCC No.60605 in Simulated Gastrointestinal Tract

[0086] 1 Test method

[0087] (1) Preparation of simulated gastric juice: Dissolve pepsin in sterilized physiological saline (0.9% w / v, adjust pH to 3.0 with hydrochloric acid) so that the final concentration is 3 g / L. Filter through a 0.22μm sterile filter membrane and prepare for immediate use.

[0088](2) Configuration of simulated intestinal juice: dissolve trypsin in sterilized physiological saline (0.9% w / v, adjust pH to 8.0 with NaOH) to make the final concentration 1g / L, and add bile salt to make the final concentration 0.3 %. Filter through a 0.22μm sterile filter membrane and prepare for immediate use.

[0089] (3) Lactobacillus brevis GDMCC No.60605 was continuously activated for three generations (18 hours each time) before the experiment. Determination of the continuous activation of three generations of strains in A 600 The OD val...

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Abstract

The invention discloses a lactobacillus brevis capable of relieving anxiety and improving sleep and application of the lactobacillus brevis, and belongs to the technical field of microorganisms. The lactobacillus brevis GDMCC No.60605 can survive well in simulated gastrointestinal tract and has excellent cell adhesion and GABA production abilities. In an open-field experiment, the frequency of mice entering a central area can be significantly increased, and the time of the mice staying n the open-field central area is prolonged without affecting the total movement distance of the mice. In a high plus maze test, the number of times of the mice entering open arms can be significantly increased, the pentobarbital sodium induced sleep time can be prolonged, the pentobarbital sodium induced sleep latency can be shortened, the intestinal flora can be adjusted, and the relative abundance of Allobaculum in the intestines of the mice is significantly increased. The lactobacillus brevis GDMCC No.60605 is used for preparing a pharmaceutical composition and a fermented food capable of relieving anxiety and improving sleep, and has a very wide application prospect.

Description

technical field [0001] The invention relates to Lactobacillus brevis capable of relieving anxiety and improving sleep and uses thereof, belonging to the technical field of microorganisms. Background technique [0002] Insomnia is a subjective experience in which sleep quality or time does not meet normal physiological needs due to difficulties in falling asleep and maintaining sleep. It is the most common sleep disorder disease. A number of clinical symptoms are commonly used to diagnose insomnia, including: difficulty falling asleep, waking up at night and difficulty falling back asleep, waking up early the next day, and nonrestorative sleep. Insomnia is one of the most prevalent afflictions in today's society, based on incidence and demographics. In my country, at least 35% of people suffer from insomnia, 17% of people suffer from severe insomnia symptoms, and the proportion of people whose sleep is limited to "light sleep" is as high as 77.3%. Insomnia can lead to lack ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23C11/06A23L11/00A23L19/20A23L29/00A23L33/135A61K35/747A61P25/22A61P25/20C12R1/24A23L11/50A23L11/60
CPCC12N1/20A23L29/065A23L33/135A23L19/20A23C9/1234A23C11/06A61K35/747A61P25/22A61P25/20A23V2002/00A23L11/50C12R2001/24C12N1/205A23V2400/121A23V2200/322A23V2200/3204
Inventor 田丰伟陈卫翟齐啸韩啸于雷雷赵建新张灏
Owner JIANGNAN UNIV
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