Mouse thyroid epithelial cell isolation and culture method
A technology of epithelial cells and culture methods, applied in the field of cell biology, can solve the problems of large differences in survival time and function of thyroid cells, and high cost, and achieve the effects of saving digestion time, reducing dosage, and improving survival rate
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[0019] In order to present the purpose and advantages of the present invention more clearly, specific embodiments will now be further described. The specific embodiments described here are only for explaining the present invention, and are not intended to limit the present invention.
[0020] In the present invention, male mice aged 6-8 weeks are selected to separate thyroid epithelial cells. The specific operation is as follows:
[0021] 1. Take 6-8 week-old male mice, kill them by intraperitoneal injection of pentobarbital sodium, fix the mice, sterilize them with 75% alcohol, take out the mouse thyroid tissue connected to the trachea, and place them in pre-cooled sterile Soak and wash in PBS buffer containing double antibody, and peel off thyroid tissue;
[0022] 2. Quickly remove the capsule, blood cells, connective tissue and other attachments on the thyroid gland under a microscope on ice, and transfer the peeled thyroid gland to a new PBS buffer solution for washing t...
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