Experimental method for injecting repeating autologous blood clots in dogs

An experimental method and autologous blood technology, applied in medical science, veterinary instruments, etc., can solve the problems of insufficient precision, complicated operation, and insignificant effect, and achieve the effect of suitable cost and convenient operation.

Inactive Publication Date: 2014-03-05
王景文
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Problems solved by technology

However, in the dog experiment of pulmonary embolism, how to repeatedly inject autologous blood clots has always been the biggest problem in the laboratory, because the existing methods generally have problems such as complicated operation, insufficient precision, and ineffective effects.

Method used

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Embodiment Construction

[0008] An experimental method of repeatedly injecting autologous blood clots into dogs. First, healthy mixed-breed dogs of any sex and with a body weight of 13 kg were selected. All dogs were anesthetized by ear vein injection of 50 g / L pentobarbital sodium at a dose of 0.6 mL / kg; after anesthesia, the supine position was fixed on the operating table; the endotracheal tube was inserted into the trachea for fixation; the neck clipped with iodophor was disinfected, the external jugular vein was exposed, and 50 mL of blood was collected under sterile conditions, and 400 U of thrombin was added to a plate Mix well, and let it stand at room temperature for about 2 hours to form a thrombus; cut the thrombus into a cylindrical shape with a diameter of 2-4 mm for later use; cut the external jugular vein, insert a standard 6F three-lumen floating catheter, and insert another incision to the depth of the catheter About 15-20 cm to the right atrium, a sensor is connected to detect the pul...

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Abstract

The invention discloses an experimental method for injecting repeating autologous blood clots in dogs. Firstly, healthy crossbred dogs are selected with no regard to gender, wherein the body mass of each dog ranges from 13kg to 19kg, and all the dogs are subjected to a pentobarbital sodium ear edge intravenous injection for anesthesia; after anesthesia, the dogs are fixed on an operating table at a supine position; a trachea cannula is inserted in a trachea and fixed; hair on the neck of each dog is cut, iodophor is applied to the neck for disinfection, the external jugular vein of each dog is exposed, 50mL of blood is collected under an aseptic condition, 200-400U thrombin is added into the blood, and the thrombin and the blood are mixed in a flat vessel and stand for 2 hours at a room temperature to form a thrombus; the thrombus is cut into a cylinder shape with the diameter of 2-4 millimeters as a standby; the external jugular vein of each dog is cut open, a standard 6F three-cavity floating catheter is inserted in the external jugular vein, a catheter is inserted in another incision to the depth of about 15-20 centimeters to reach the right atrium of the dog, and the catheter is connected to a sensor for detection of pulmonary artery pressure. The experimental method for injecting the repeating autologous blood clots in the dogs has the advantages that researches about changes in circulation power in a human body can be more accurate, operation is convenient, and cost is proper.

Description

technical field [0001] The invention relates to a method for experimenting on dogs, in particular to an experiment method for repeatedly injecting autologous blood clots into dogs. Background technique [0002] The choice of dog as the experimental subject is due to the full consideration of the dog's characteristics. First, it belongs to the double circulation like humans, with independent pulmonary circulation and systemic circulation. The pulmonary artery pressure of the two is in the same order of magnitude. In terms of physiology, we generally think that humans and dogs belong to the type of sympathetic tension, which is very different from rabbits. At the same time, choosing a dog as an experimental model can more accurately study changes in the dynamics of the human pulmonary circulation. However, how to repeatedly inject autologous blood clots has always been the biggest problem in the laboratory when conducting the dog experiment of pulmonary embolism, because the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00
Inventor 王景文
Owner 王景文
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