Experimental method for injecting repeating autologous blood clots in dogs
An experimental method and autologous blood technology, applied in medical science, veterinary instruments, etc., can solve the problems of insufficient precision, complicated operation, and insignificant effect, and achieve the effect of suitable cost and convenient operation.
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[0008] An experimental method of repeatedly injecting autologous blood clots into dogs. First, healthy mixed-breed dogs of any sex and with a body weight of 13 kg were selected. All dogs were anesthetized by ear vein injection of 50 g / L pentobarbital sodium at a dose of 0.6 mL / kg; after anesthesia, the supine position was fixed on the operating table; the endotracheal tube was inserted into the trachea for fixation; the neck clipped with iodophor was disinfected, the external jugular vein was exposed, and 50 mL of blood was collected under sterile conditions, and 400 U of thrombin was added to a plate Mix well, and let it stand at room temperature for about 2 hours to form a thrombus; cut the thrombus into a cylindrical shape with a diameter of 2-4 mm for later use; cut the external jugular vein, insert a standard 6F three-lumen floating catheter, and insert another incision to the depth of the catheter About 15-20 cm to the right atrium, a sensor is connected to detect the pul...
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