Experimental method for injecting repeating autologous blood clots in dogs

Abstract

The invention discloses an experimental method for injecting repeating autologous blood clots in dogs. Firstly, healthy crossbred dogs are selected with no regard to gender, wherein the body mass of each dog ranges from 13kg to 19kg, and all the dogs are subjected to a pentobarbital sodium ear edge intravenous injection for anesthesia; after anesthesia, the dogs are fixed on an operating table at a supine position; a trachea cannula is inserted in a trachea and fixed; hair on the neck of each dog is cut, iodophor is applied to the neck for disinfection, the external jugular vein of each dog is exposed, 50mL of blood is collected under an aseptic condition, 200-400U thrombin is added into the blood, and the thrombin and the blood are mixed in a flat vessel and stand for 2 hours at a room temperature to form a thrombus; the thrombus is cut into a cylinder shape with the diameter of 2-4 millimeters as a standby; the external jugular vein of each dog is cut open, a standard 6F three-cavity floating catheter is inserted in the external jugular vein, a catheter is inserted in another incision to the depth of about 15-20 centimeters to reach the right atrium of the dog, and the catheter is connected to a sensor for detection of pulmonary artery pressure. The experimental method for injecting the repeating autologous blood clots in the dogs has the advantages that researches about changes in circulation power in a human body can be more accurate, operation is convenient, and cost is proper.

Description

technical field [0001] The invention relates to a method for experimenting on dogs, in particular to an experiment method for repeatedly injecting autologous blood clots into dogs. Background technique [0002] The choice of dog as the experimental subject is due to the full consideration of the dog's characteristics. First, it belongs to the double circulation like humans, with independent pulmonary circulation and systemic circulation. The pulmonary artery pressure of the two is in the same order of magnitude. In terms of physiology, we generally think that humans and dogs belong to the type of sympathetic tension, which is very different from rabbits. At the same time, choosing a dog as an experimental model can more accurately study changes in the dynamics of the human pulmonary circulation. However, how to repeatedly inject autologous blood clots has always been the biggest problem in the laboratory when conducting the dog experiment of pulmonary embolism, because the ...

AI Technical Summary

Problems solved by technology

However, in the dog experiment of pulmonary embolism, how to repeatedly inject autologous blood clots has always been the biggest problem in the laboratory, because the existing methods generally have problems such as complicated operation, insufficient precision, and ineffective effects.