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Method for producing cerebral ischemia-reperfusion injury animal model

A technology of cerebral ischemia reperfusion and animal models, which is applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of complicated production and low success rate, and achieve simple operation, high success rate and easy operation Effect

Inactive Publication Date: 2016-02-17
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing animal models of cerebral ischemia and reperfusion are complicated to make and have a low success rate.

Method used

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Examples

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Effect test

Embodiment 1

[0019] In an embodiment of the present invention, a method for making an animal model of cerebral ischemia-reperfusion injury comprises the following steps:

[0020] 1) Anesthesia: the mice were fasted for 12 hours before the operation, and they were free to drink water. The mice were anesthetized with pentobarbital sodium in the neck, and each kilogram of mice was given 45 mg of pentobarbital sodium; Bital sodium.

[0021] 2) Cerebral ischemia: fix the mouse on the brain stereotaxic apparatus, cut the skin on both sides of the neck of the mouse, separate the bilateral common carotid arteries and vagus nerves, cut off the bilateral common carotid arteries, and insulate the blood supply area of ​​the middle cerebral artery of the mice. Blood continued for 2.5 hours to prepare animals with cerebral ischemia injury.

[0022] 3) Reperfusion after cerebral ischemia: after cutting off the bilateral common carotid arteries for 2 hours, connect the bilateral common carotid arteries w...

Embodiment 2

[0025] In an embodiment of the present invention, a method for making an animal model of cerebral ischemia-reperfusion injury comprises the following steps:

[0026] 1) Anesthesia: The rabbits were fasted for 12 hours before the operation, and they were free to drink water. The rabbits were anesthetized with pentobarbital sodium, and each kilogram of rabbits was given 50 mg of pentobarbital sodium; Bital sodium.

[0027] 2) Cerebral ischemia: the rabbit was fixed on a stereotaxic instrument, the skin on both sides of the rabbit's neck was cut open, the bilateral common carotid arteries and vagus nerves were separated, and a balloon catheter was implanted in the bilateral common carotid arteries. The process of implanting the balloon catheter in the rabbit is as follows: bluntly dissect the common carotid artery of the experimental body, ligate the common carotid artery, cut a small opening 2-3 mm below the ligation point, and insert the balloon catheter into the small opening....

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Abstract

The invention discloses a method for producing a cerebral ischemia-reperfusion injury animal model. The method comprises the steps of conducting neck anaesthesia on an experimental animal with pentobarbital sodium, cutting out skin at the two sides of the neck of the experimental animal, separating bilateral arteria carotis communis and pneumogastric nerves, cutting off the bilateral arteria carotis communis or implanting a balloon catheter in the bilateral arteria carotis communis, inflating air into the implanted balloon catheter till the balloon catheter blocks the starting portion of the bilateral arteria carotis communis in the brain, keeping the ischemic state for 2.5-3 hours, and obtaining a cerebral ischemia injury animal; when two hours pass after the bilateral arteria carotis communis is cut off, using a hose for connecting the bilateral arteria carotis communis, conducting suturing, and infusing blood into the hose; or discharging gas out of the balloon catheter, and pouring blood into an ischemic zone once more; conducting neurologic impairment scoring within different time according to requirements, and making records with a video recorder. The method is easy and convenient to operate, the success rate is high, and the reasonable model is provided for cerebral ischemia and reperfusion.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a method for making an animal model of cerebral ischemia-reperfusion injury. Background technique [0002] Animal disease models are mainly used in experimental physiology, experimental pathology and experimental therapeutics (including new drug screening) research. The development of human diseases is very complicated. Using humans as experimental objects to deeply explore the mechanism of disease occurrence and promote the development of medicine has come slowly. The accumulated clinical experience is not only limited in time and space, but also many experiments are morally unreasonable. and methods are also limited. With the help of indirect research on animal models, factors that are impossible or difficult to exclude under natural conditions can be consciously changed, so as to observe the experimental results of the model more accurately and conduct comparative studi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D7/00
Inventor 侯软玲李颖虹王国红魏林郁郭直岳赵红岗李东亮
Owner XINXIANG MEDICAL UNIV
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