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Construction method of spleen deficiency of vital energy SHR animal model

A construction method and animal model technology, applied in the direction of pharmaceutical formulations, preparations for in vivo experiments, etc., can solve problems that have attracted sufficient attention

Inactive Publication Date: 2008-06-18
LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity and particularity of the collection of animal symptoms and signs, an ideal spleen deficiency syndrome model is required. Not only the symptoms and signs are the same or similar, but also it is hoped that it can be accurately expressed by scientific, microscopic, and quantitative objective indicators, and can It can stand the test of prescription (medicine) testing (Wang Caixia. On the thinking of animal model research in traditional Chinese medicine. Chinese Medical Journal, 1997, 12(3): 45-47), and the work in this area has not attracted enough attention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Select 36 SD rats, half male and half female, weigh the rats respectively, inject 1.5mL 3% pentobarbital sodium solution 1.5mL per 100g body weight intraperitoneally to anesthetize the SD rats, and disinfect them with bromogeramine tincture; make a midline incision into the abdomen Lift the cecum (about 60mm in length and about 10mm in diameter), and after ligating the ileocolic blood vessels, fan the mesentery, clamp the distal and proximal ends of the intestine to be resected with arterial clips, and resect the ileocolic branch of the anterior mesenteric artery and the first right colic branch of the anterior mesenteric artery in a wedge shape 1. The ileocolonic tube where the branches are distributed, including the cecum between the small intestine and the colon (according to this standard, the length of the resected ileum segment is about 400 mm, and the length of the colon segment is about 50 mm). End-to-end anastomosis was performed, and the incision was sutured; t...

Embodiment 2

[0018] Select 36 SD rats, half male and half female, weigh the rats respectively, inject 1.5mL 3% pentobarbital sodium solution 1.5mL per 100g body weight intraperitoneally to anesthetize the SD rats, and disinfect them with bromogeramine tincture; make a midline incision into the abdomen Lift the cecum (about 60mm in length and about 10mm in diameter), and after ligating the ileocolic blood vessels, fan the mesentery, clamp the distal and proximal ends of the intestine to be resected with arterial clips, and resect the ileocolic branch of the anterior mesenteric artery and the first right colic branch of the anterior mesenteric artery in a wedge shape 1. The ileocolon intestine distributed by the second branch, including the cecum between the small intestine and the colon (according to this standard, the length of the resected ileum segment is about 400 mm, and the length of the colon segment is about 150 mm). The colon was anastomosed end to end, and the incision was sutured;...

Embodiment 3

[0020]Forty-eight SD rats, half male and half male, were randomly divided into four groups. There were 12 rats in the sham operation group (opening the abdomen and closing the abdomen without resection of the intestines), 12 rats in the Qi deficiency syndrome model group, 12 rats in the Sijunzi Decoction treatment group (modeling + Sijunzi Decoction treatment, referred to as the Sijunzi Decoction group) and 12 rats in the Pulsatilla Decoction treatment group. (Modeling + Pulsatilla Decoction treatment, referred to as Pulsatilla Decoction group) 12 rats. Weigh the body weight of the rat, inject 1.5mL 3% pentobarbital sodium solution 1.5mL per 100g body weight intraperitoneally, anesthetize, disinfect with bromogeramine tincture; make a midline incision into the abdomen, lift the cecum (about 60mm in length, about 10mm in diameter), and ligate it back After the colonic blood vessels, the mesentery is fan-shaped, and the distal and proximal ends of the intestine to be resected ar...

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Abstract

The invention discloses an animal-model construction method for screening the medicine for treating spleen-qi deficiency syndrome and appraising method for treating spleen-qi deficiency syndrome. The invention includes the following steps: first select an appropriate rat, weigh the rat, with every 100g body weight injected 1.5mL 3% sodium pentobarbital solution, after the anesthesia, disinfect the rat with benzalkonium bromide; incise in the middle and enter abdomen, lift the cecum, ligate the ileocolic blood vessel, incise the messentery in a fan-shaped manner, clip the far and near ends of the drafted-resecting intestinal canal with an artery haptor, resect in a spheonoid manner the ileocolon branch of the anterior mesenteric artery and the ileocolon canal where the first branch, or the first branch and the second branch of the right colic branch are distributed, including the cecum between the small intestine and the colon, disinfect with benzalkonium bromide, inosculate the ileum end and the colon end, suture the incision, breed the rat in a single cage. The animal model constructed according to the invention, with the same or similar clinic symptom and physical sigh, is of good repeatability and stability and can withstand the test of syndrome differentiation by formula (drug) effect assessment.

Description

technical field [0001] The invention belongs to the technical field of medical evaluation and detection, and in particular relates to a method for constructing an animal model for screening and treating drugs for spleen-qi deficiency syndrome and evaluating methods for treating spleen-qi deficiency syndrome. Background technique [0002] The term spleen deficiency in traditional Chinese medicine first appeared in the "Neijing", and it was found in "Lingshu. Tiannian": "Seventy years old, spleen deficiency, skin dry." See also "Lingshu. Benshen": "Spleen deficiency is The limbs are not used, and the internal organs are restless." The main physiological functions of the spleen are to govern the movement and transformation, promote clearness, control blood, and govern the muscles of the limbs. The function of the spleen is a synthesis of multi-system functions with the main function of the digestive system (Chen Yixin, Chen Jiaxu, Ji Shaoliang. A theoretical discussion on the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
Inventor 章学林张静喆顾宏刚沈平
Owner LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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