Method for separating and culturing primary human esophageal epithelial cells
A technology of epithelial cells and culture methods, applied in the field of cell culture of modern biotechnology, can solve the problems of restricting research work, unstable methods, expensive reagents, etc., and achieve the effect of reliable cell resources, simple and easy-to-master technology, and easier preservation
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[0028] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.
[0029] The present invention will be described in detail below in conjunction with the accompanying drawings and examples, and the protection content of the present invention is not limited to the following examples.
[0030] In the following examples, various procedures and methods not described in detail are conventional methods well known in the art.
[0031] The experimental instruments and reagents used in this experiment are as follows:
[0032] A set of surgical instruments, an inverted microscope (XDS-1A, Shanghai), a fluorescence microscope (Leica, the United States), a constant temperature incubator (Heraeus, the United States), a low-temperature centrifuge (TD24B-WS, Shanghai), an ultra-low temperature refrigerator (Zhongke Meiling ), pipette gun (Eppendorf, U.S.), electronic analytical balance (Sartorius, U.S.),...
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